Eksplorasi dan Karakteristik Bakteri Endofit Asal Tanaman Handeuleum (Graptophyllum pictum (L) Griff)

Jean Nihana Manalu, Maya Mariana

Abstract


Handeuleum (Graptophyllum pictum L. Griff) is a plant that is used as a medicinal plant with secondary metabolite compounds so that it can overcome several diseases. Exploration of endophytic bacteria in handeuleum plants needs to be done, because the content of secondary metabolites can be used to suppress the growth of plant pathogens.The purpose of this study was to obtain isolates of endophytic bacteria and find out their characteristics. This research was conducted with an exploratory method that began with the isolation of endophytic bacteria and several tests were carried out such as hemolysis activity, hypersensitivity, gram test, chitinolytic test, phosphate solvent activity, and antagonist test.The isolation results obtained a high population of endophytic bacterial isolates, namely in the roots, as many as 8 isolates that are pathogenic in mammals, hypersensitivity tests showed no isolates that were pathogenic in plants, gram tests obtained as many as 24 isolates that were gram-positive, as many as 5 isolates were able to lyse phosphate, no isolates could degrade chitin, as many as 2 isolates were antimicrobial against Rastolnia solanacearum indicated by the presence of clear zones and 26 isolates were able to suppress the growth of Fusarium oxysporum. Keywords: Antimicrobial, pathogenic, hemolysis, hypersensitive, chitinolytic

Full Text:

PDF

References


Agusta, A. (2009) Biologi dan Kimia Jamur Endofit. Bandung: Institut Teknologi Bandung.

Faria, D.C. et al. (2013) ‘Endophytic bacteria isolated from orchid and their potential to promote plant growth Endophytic bacteria isolated from orchid and their potential to promote plant growth’, (September). Available at: https://doi.org/10.1007/s11274-012-1173-4.

Gohel, V. et al. (2006) ‘Bioprospecting and antifungal potential of chitinolytic microorganisms’, 5(January), pp. 54–72.

Ji Hye, S., Gururani Anand, M. and Chun, S. (2013) ‘Isolation and characterization of plant growth promoting endophytic diazotrophic bacteria from Korean rice cultivars Microbiological Research Isolation and characterization of plant growth promoting endophytic diazotrophic bacteria from Korean rice cultiv’, Microbiological Research, 169(1), pp. 83–98. Available at: https://doi.org/10.1016/j.micres.2013.06.003.

Malfanova, N.V. (2013) Endophytic bacteria with plant growth promoting and biocontrol abilities. Leiden University.

Munif, A. and Wiyono, S. (2012) ‘Isolasi Bakteri Endofit Asal Padi Gogo dan Potensinya sebagai Agens Biokontrol dan Pemacu Pertumbuhan Isolation of Endophytic Bacteria from Upland Rice and Its Role as Biocontrol Agents and Plant Growth Inducer’, 8.

Pavlo, A. et al. (2011) ‘Endophytic bacteria enhancing growth and disease resistance of potato ( Solanum tuberosum L .)’, Biological Control, 56(1), pp. 43–49. Available at: https://doi.org/10.1016/j.biocontrol.2010.09.014.

Purwanto, U.M.S., Pasaribu, F.H. and Bintang, M. (2014) ‘Isolasi bakteri endofit dari tanaman sirih hijau ( Piper betle L .) dan potensinya sebagai penghasil senyawa antibakteri’, 1(1), pp. 51–57.

Resti, Z., Habazar, T. and Putra, D.P. (2013) ‘Skrining dan identifikasi isolat bakteri endofit untuk mengendalikan penyakit hawar daun bakteri pada bawang merah’, (2007).

Rosmala, A., Khumaida, N. and Sukma, D. (2015) ‘Perubahan Morfologi dan Pertumbuhan Handeuleum ( Graptophyllum pictum L . Griff ) akibat Iradiasi Sinar Gamma Morphological Changes and Growth of Handeuleum ( Graptophyllum pictum L . Griff ) due to Gamma Ray Irradiation’, 43(3), pp. 235–241.

Schaad, N.W., Jones, J. and W, C. (2001) Laboratory Guide for Identification of Plant Pathogenic Bacteria. 3rd edn. American Phytopathological Society. St Paul, MN.

Skalka, B., Smola, J. and Pillich, J. (1979) ‘A simple method of detecting staphylococcal hemolysin’, Zbl. Bakteriol. Hyg. I. Abt. Orig, 245(3), pp. 283–286.

Sonia, A.V. and Setiawati, T.C. (2022) ‘Aktivitas bakteri pelarut fosfat terhadap peningkatan ketersediaan fosfat pada tanah masam The activity of phosphate solubilizing bacteria on increasing phosphate available in acid soil’, 15(1), pp. 44–53.

Suhandono, S. and Utari, B.I. (2014) ‘Isolation and Molecular Identification of Endophytic Bacteria from Durian Isolation and Molecular Identification of Endophytic Bacteria from the Arils of Durian ( Durio zibethinus Murr .) var . Matahari’, Microbiology Indonesia, 8, pp. 161–169. Available at: https://doi.org/10.5454/mi.8.4.3.

Sukmadewi, D.K.T. et al. (2017) ‘Test of Phytopathogenicity , Hemolysis and Microbial Ability in Solubilizing Phosphate and Potassium’, 19(2), pp. 68–73.

Syamsuhidayat, Sri Sugati Hutapea, J.R. (1991) Inventaris Tanaman Obat Indonesia. Jakarta: Departemen Kesehatan RI, Badan Penelitian dan Pengembangan Kesehatan.

Tian, B., Cao, Y. and Zhang, K. (2015) ‘Metagenomic insights into communities , functions of endophytes , and their associates with infection by root-knot nematode , Meloidogyne incognita ’, Nature Publishing Group, (October), pp. 1–15. Available at: https://doi.org/10.1038/srep17087.

Wahyudi, A.T., Meliah, S. and Nawangsih, A.A. (2011) ‘Xanthomonas oryzae pv. oryzae bakteri penyebab hawar daun pada padi: Isolasi, karakterisasi, dan telaah mutagenesis dengan transposon’, 15(1).

Wahyuni, S., Kirami, M.W. and Khaeruni, A. (2014) ‘Karakterisasi sifat biokimia isolat bakteri kitinolitik asal tambal udang’, Biologi Eksperimen dan Keanekaragaman Hayati, 2(2), pp. 50–55.

Zakiyah, A., Radiastuti, N. and Sumarlin, L.O. (2015) ‘Aktivitas antibakteri kapang endofit dari tanaman kina (Cinchona calisaya Wedd .)’, 8, pp. 51–58.

Zimbro, M.J. (2009) Difco & BBL Manual: Manual of Microbiological Culture Media. 2nd edn. Becton, Dickinson and Company, Sparks, Md.

Zubaidah, E., Saparianti, E. and Purwohadisantoso, K. (2009) ‘Isolasi bakteri asam laktat dari sayur kubis yang memiliki kemampuan penghambatan bakteri patogen (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, dan Salmonella thypimurium)’, Jurnal Teknologi Pertanian, 10(1), pp. 19–27.




DOI: https://doi.org/10.36987/agroplasma.v10i1.4188

Refbacks

  • There are currently no refbacks.


Jurnal Agroplasma

Program Studi Agroteknologi
Fakultas Sains dan Teknologi Universitas Labuhanbatu
JL. SM. Raja No. 126-A Km. 3,5 Aek Tapa Telp./Fax. (0624) 21901 Rantauprapat Kab. Labuhanbatu – Sumatera Utara Pos. 21415
Email : agroplasma@ulb.ac.id

Creative Commons License

All publications by Jurnal Agroplasma [p-ISSN: 2303-2944] [E-ISSN:2715-033X] is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.