Multiplikasi Tunas Timun Apel (Cucumis sp.) dengan Menggunakan NAA dan Kinetin Secara In Vitro

Ratih Sartika, Nurcahyo Widyodaru Saputro, Ani Lestari

Abstract


To support the productivity of apple cucumber, non-conventional breeding efforts are carried out using plant tissue culture method. Research has been conducted on apple cucumber tissue culture with the target of direct organogenesis from leaf cut explants on aseptically grown sprouts in the laboratory. The aim was to find out as well as get a combination of NAA and Kinetin concentrations that affect the multiplication stage of apple cucumber shoots, namely to induce the emergence of shoots by direct organogenesis. The application of growth regulators NAA and Kinetin on MS culture media influence on the growth of apple cucumber shoots with leaf cut explants. The results showed that the optimal percentage value of explants in forming buds was obtained in MS media with the addition of 0.20 ppm NAA and 1,68 ppm which was 50%. While the fastest shoot emergence time was obtained by treatment on MS media with the addition of 0.15 ppm NAA and 2.18 Kinetin. The treatment on MS media with the addition of 0.15 ppm NAA and 2.18 Kinetin was also superior in the shoot height parameter with an average shoot height of 1 cm.

 

Keywords: kinetin, plant tissue culture, MS culture media, NAA, apple cucumber


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References


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DOI: https://doi.org/10.36987/agroplasma.v12i1.6576

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