Isolasi Jamur Pengurai Selulosa pada Tanah Hutan Dikawasan Geopark Ranah Minang Silokek sebagai Dekomposer

Atika Mariana, Santi Diana Putri, Resti Fevria, Kiki Amelia

Abstract


This research is based on the need to utilize natural organisms such as cellulose-degrading fungi to support sustainable agriculture and organic waste management in the Ranah Minang Silokek Geopark. This study aims to identify and isolate cellulose-degrading fungi from forest soils in the area, as well as evaluate their potential use to improve soil fertility and reduce dependence on synthetic chemicals. The research methods included soil sampling from several locations in the Silokek Geopark forest, isolation of fungi using Potato Dextrose Agar (PDA) media and purified on Carboxymethyl Cellulose (CMC) selective medium, macroscopic identification of fungal morphology, and testing of cellulolytic activity by observing clear zones as an indicator of the ability of fungi to degrade cellulose. The results obtained 12 isolates that have the potential as biodecomposers in decomposing cellulose. The best isolate was found in DG3 nagari Durian Gadang with a clear zone of 0.1 cm with a colony count of 19 x 107 CFU/g on selective CMC medium. In conclusion, the soil in the Ranah Minang Silokek Geopark has high potential as a source of cellulose-degrading fungi that can be used for environmentally friendly agricultural applications, such as composting and biofertilizers, so as to increase soil fertility naturally.

 

Keywords:  silokek geopark, isolation, decomposer, cellulose, forest soil


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References


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DOI: https://doi.org/10.36987/agroplasma.v12i1.7374

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