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Isolation and Identification of Endophytic Bacteria from Euphorbia hirta L. (Patikan Kebo)


 
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1. Title Title of document Isolation and Identification of Endophytic Bacteria from Euphorbia hirta L. (Patikan Kebo)
 
2. Creator Author's name, affiliation, country Rindiyani Rindiyani; Universitas Bengkulu; Indonesia
 
2. Creator Author's name, affiliation, country Ratu Intan Anugrah; Universitas Bengkulu; Indonesia
 
2. Creator Author's name, affiliation, country Bella Nabila; Universitas Bengku; Indonesia
 
2. Creator Author's name, affiliation, country Sisilia Salsabilla; Universitas Bengkulu; Indonesia
 
2. Creator Author's name, affiliation, country Elia Susanti; Universitas Bengkulu; Indonesia
 
2. Creator Author's name, affiliation, country Desi Rofiani; Universitas Bengkulu; Indonesia
 
2. Creator Author's name, affiliation, country Yolanda Puspytha; Universitas Bengkulu; Indonesia
 
2. Creator Author's name, affiliation, country Risky Hadi Wibowo; Universitas Bengkulu; Indonesia
 
2. Creator Author's name, affiliation, country Sipriyadi Sipriyadi; Universitas Bengkulu; Indonesia
 
2. Creator Author's name, affiliation, country Welly Darwis; Universitas Bengkulu; Indonesia
 
3. Subject Discipline(s) Microbiology
 
3. Subject Keyword(s) Endophytes Bacteria; Euphorbia hirta; Identification; Isolation
 
4. Description Abstract

Patikan Kebo (Euphorbia hirta L.) is a plant that lives scattered in the yard, has potential as an antibacterial, one way to extract plants is by isolating endophytic bacteria. Endophytes are bacteria found in plant tissue. The purpose of this study was to isolate and identify the morphology of endophytic bacteria from  plant Patikan Kebo (Euphorbia hirta L.). The method used is the Total Plate Count (TPC) method by taking samples, isolating bacteria, pure culture, morphological identification, and Gram staining. Endophytic bacteria were obtained by isolating parts of the plant Patikan Kebo (E. hirta L.), namely the roots of patikan kebo (PRE), Patikan kebo stem endophyte (PSE) and Patikan kebo leaves endophyte (PLE). The results of the study found 22 isolates, endophytic bacterial isolates have diverse morphological characteristics including appearance, elevation, edges and color. There are 20 isolates of endophytic bacteria that belong to the Gram positive group and 2 isolates of endophytic bacteria that belong to the Gram negative group. It can be concluded that endophytic bacterial isolates from Patikan Kebo (Euphorbia hirta L.) plants have different morphologies

 
5. Publisher Organizing agency, location Universitas Labuhanbatu
 
6. Contributor Sponsor(s) Directorate of Learning and Student Affairs (Belmawa), Ministry of Education, Culture, Research and Technology (Kemdikbudristek) through the Scientific Article Student Creativity Program (PKM-AI) incentive in 2024
 
7. Date (YYYY-MM-DD) 2025-03-22
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier https://jurnal.ulb.ac.id/index.php/nukleus/article/view/6688
 
10. Identifier Digital Object Identifier (DOI) https://doi.org/10.36987/jpbn.v11i1.6688
 
11. Source Title; vol., no. (year) JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS; Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
 
12. Language English=en en
 
13. Relation Supp. Files
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
 
15. Rights Copyright and permissions Copyright (c) 2025 Rindiyani Rindiyani, Ratu Intan Anugrah, Bella Nabila, Sisilia Salsabilla, Elia Susanti, Desi Rofiani, Yolanda Puspytha, Risky Hadi Wibowo, Sipriyadi Sipriyadi, Welly Darwis
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