Banana plant (Musa acuminata) is a type of fruit horticulture plant that is currently widely cultivated by the community, in the cultivation of banana plants can not be separated from pests and diseases. Fusarium oxysporum is one of the plant-disrupting fungi that can infect plants to cause wilt disease, this disease is the most dangerous disease that attacks banana plants and is one of the most feared diseases, especially by horticultural farmers because it has the potential to cause large losses, therefore it is necessary to apply PGPR (Plant Growth Promoting Rhizobacteria) which acts as a biocontrol in the form of biopesticides to control pests and plant pathogens has been considered a good practice to maintain agroecosystems. This study was conducted to see the growth of Fusarium which will then be calculated using the Koch postulate method to see the development of the number of Fusarium oxyporum colonies. One of the problems that cause inhibition of fusarium growth is air temperature and humidity. The results obtained based on that the method used is able to reduce the population of Fusarium at dilution 10⁶.

Keywords: banana plants, fusarium oxysporum, koch postulate, PGPR

Aryantha, I.N.Y.P., P.L. Dian. & P.D.P. Nurmi. (2004). Potensi isolat bakteri penghasil IAA dalam peningkatan pertumbuhan kecambah kacang hijau pada kondisi hidroponik. Jurnal Mikrobiologi Indonesia, 9: 43-46.

Budilaksono, M. (2020). Sepuluh negara pengekspor pisang terbesar di dunia. https://hortikultura.sariagri.id/56082/sepuluh-negara-pengekspor-pisang-terbesar-di-dunia. Diunduh 03 Maret 2022.

Das, S., Nurunnabi, T. R., Parveen, R., Mou, A. N., Islam. E., Islam, K. M. D. (2019). Isolation and characterization of indole acetic acid producing bacteria from rhizosphere soil and their effect on seed germination. International Journal Of Current Microbiology And Applied Sciences, 8(03), 2319-7706.

Jumjunidang, Hermanto C, & Riska. (2011). Virulensi isolat Fusarium Oxysporum f.sp. cubense VCG 01213/16 pada pisang barangan dari varietas pisang dan lokasi yang berbeda. J. Hort. 21(2): 145–151.

Martiansyah, I. (2021). Petunjuk teknis budidaya pisang asal kultur in vitro dengan teknologi PPBBI. Puslit Bioteknologi dan Bioindustri Indonesia (PPBBI), PT Riset Perkebunan Nusantara. 13 Hal.

Mishra J, Tewari S, Singh S, Arora NK .(2015). Biopesticides where we stand? In: Arora NK (ed) Plant Microbe Symbiosis: Applied Facets. Springer, New Delhi, pp 37–75

Palmero D., Iglesias C., De Cara M., Lomas T., Santos M. & Tello J.C. (2009) Species of Fusarium isolated from river and sea water of Southeastern Spain and Pathogenicity On Four Plant Species. Plant Diseases 93, 377–385.

Porter, L.D., Pasche, J.S., Chen, W., & Harveson, R.M. (2015). Isolation, identification, storage, pathogenicity tests, hosts, and geographic range of Fusarium Solani F.Sp. Pisi Causing Fusarium Root Rot of Pea. Plant Health Progress, 16(3): 136-145. Doi: 10.1094/PHP-DG-15-0013.

Saraswati, R., E. Husen & R.D.M. Simanungkalit. ( 2007). Metode analisis biologi tanah. Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian. Jawa barat. 271 hal.

Sinaga F, Sritamin M & Suada I.K. (2021). Pembentukan muatan Hipovirulen Fusarium Oxysporum F.SP Cubense penyebab layu pada tanaman pisang (Musa paradisiaca L.) menggunakan asam nitrat. Vol 1, No 1, Hal 1-10.

Sriwahyuni, S., Oktarina, H., & Chamzurni, T. (2023). Pengaruh bioaktivator dalam pupuk organik cair kulit pisang untuk mengnendalikan penyakit layu Fusarium pada tanaman tomat (Solanum lycopersicum). Jurnal Ilmiah Mahasiswa Pertanian, 8(1), 438-452

Steinberg C., Laurent J., Edel-Hermann V., Barbezant M., Sixt N., Dalle F., Aho S., Bonnin A., Hartemann P. & Sautour M. (2015) Adaptation of Fusarium Oxysporum and Fusarium Dimerum to the specific aquatic environment provided by the water systems of hospitals. Water Research 76, 53–65

Suswati, Indrawati A & Putra, D.P. (2015). Penapisan limbah pertanian (sabut kelapa dan arang sekam) dalam peningkatan ketahanan bibit pisang barangan bermikroza terhadap blood disease bacterium dan Fusarium Oxysporum F.SP Cubense. Jurnal HPT Tropika. Vol. 15, No 1:81-88.

Sopialena. (2015). Ketahanan beberapa varietas tomat terhadap penyakit Fusarium oxysporum dengan pemberian Trichoderma sp. Jurnal Agrifor. 14(1)

Swathi J., Sowjanya K.M., Narendra K. & Satya A.K. (2013). Bioactivity assay of an isolated marine Fusarium Sps. International Journal Of Bio-Science Bio-Technology 5, 179–186.

Tarigan, F. (2019). Sistem pakar mendiagnosa penyakit layu fusarium pada tanaman pisang dengan menggunakan metode certainty factor. Informasi dan Teknologi Ilmiah (INTI), 6(2), 224-228.