Perbanyakan Tunas Timun Apel (Cucumis sp.) dengan Penambahan Kombinasi BAP dan IAA pada Media Gamborg B5

Salsa Aulia Yumna Nur Azizah, Nurcahyo Widyodaru Saputro, Ani Lestari

Abstract


One of the fruits cultivated in Karawang, West Java is apple cucumber (Cucumis sp.). Apple cucumber has a fairly close kinship with melon and cucumber plants, because anatomically and morphologically apple cucumber plants are similar to melons and cucumbers, but in Karawang itself there are still many who do not know about apple cucumber fruit because the productivity of apple cucumber plants is still relatively low, which is 27 tons/ha, so it is necessary to find alternatives to obtain good apple cucumber seeds that can be developed widely. One technique that is expected to be used for the propagation of apple cucumber seeds is the tissue culture technique. The purpose of this study was to determine the most effective concentration of BAP and IAA in Gamborg B5 media in increasing the propagation of apple cucumber (Cucumis sp.) shoots in vitro. The explants used came from apple cucumber leaf tips, the media used was Gamborg B5. The results of the study showed that the growth of apple cucumber shoots in the best treatment of BAP and IAA was in treatment B with a concentration of BAP 1.0 ppm + IAA 0.1 ppm which resulted in a shoot formation time of 3 HSI and a shoot height of 1 cm. This study needs to be continued with different ZPT concentrations but in the same media and room conditions should be regulated in temperature and humidity according to the type of plant to reduce the vapor formed.

 

Keywords: Cucumis sp., IAA, BAP, gamborg, shoots


Full Text:

PDF

References


Dzaroini, R. A. (2019). Induksi Kalus Daun Mangkokan (Nothopanax Scutellarium Merr.) Menggunakan Zat Pengatur Tumbuh NAA (Naphtalene Acetic Acid) dan BAP (6-Benzyl Amino Purine) Melalui Teknik In Vitro. (Doctoral dissertation. Malang, Universitas Islam Negeri Maulana Malik Ibrahim).

Harahap, F., Hasanah, A., Insani, H., Harahap, N. K., Pinem, M. D., Edi, S., Silaban, R. (2019). Kultur Jaringan Nanas. Surabaya: Media Sahabat Cendekia.

Hartanto, A., A. Haris, and D. S. Widodo. (2009). Pengaruh Kalsium, Hormon Auksin, Giberellin dan Sitokinin terhadap Pertumbuhan dan Perkembangan Tanaman Jagung. Jurnal Kimia Sains dan Aplikasi, 12(3), 72-75. https://doi.org/10.14710/jksa.12.3.72-75

Huda, A. N., Suwarno, W. B., dan Maharijaya, D. A. (2019). Karakteristik Buah Melon (Cucumis melo L.) pada Lima Stadia Kematangan. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 46(3), 298–305. https://doi.org/10.24831/jai.v46i3.12660

Karjadi, A. K., dan Buchory, A. (2008). Pengaruh Auksin dan Sitokinin Terhadap Pertumbuhan dan Perkembangan Jaringan Meristem Kentang Kultivar Granola. Jurnal Hortikultura, 18(4).

Lathyfah, U., dan Dewi, E. R. S. (2016). Pengaruh Variasi Konsentrasi Indole Acetid Acid (IAA) Terhadap Pertumbuhan Tunas Pisang Barangan (Musa acuminata L. triploid AAA.) dalam Kultur In Vitro. Bioma: Jurnal Ilmiah Biologi, 5(1).

Lestari, E. G. (2011). Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan. Jurnal AgroBiogen, 7(1), 63. https://doi.org/10.21082/jbio.v7n1.2011.p63-68

Lidyawati, N. N., Waeniati, W., Muslimin, M., dan Suwastika, I. N. (2012). Perbanyakan Tanaman Melon (Cucumis melo L.) Secara In Vitro pada Medium MS dengan Penambahan Indole Acetic Acid (IAA) dan Benzil Amino Purin (BAP). Natural Science: Journal of Science and Technology, 1(1).

Liga N., Abadi, S., dan Saputro, N. W. (2022). Various Concentration Distribution of Benzil Amino Purine Concentration on Callus Growth in Apple Cucumber Originated from Karawang. Mangifera Edu, 6(2), 139–152. https://doi.org/10.31943/mangiferaedu.v6i2.120

Mahadi, I., Syafiâ, W., dan Agustiani, S. (2015). Kultur Jaringan Jeruk Kasturi (Citrus microcarpa) dengan Menggunakan Hormon Kinetin dan Naftalen Acetyl Acid (NAA). Dinamika Pertanian, 30(1), 37-44.

Mastuti, R. (2017). Dasar-dasar Kultur Jaringan Tumbuhan. (Universitas Brawijaya Press).

Muliati, M., Nurhidayah, T., dan Nurbaiti, N. (2017). Pengaruh NAA, BAP dan Kombinasinya Pada Media MS Terhadap Perkembangan Eksplan Sansevieria Macrophylla Secara in Vitro. JOM FAPERTA UNRI, 4(1).

Mustikawaty, P. N., dan Saputro, N. W. (2021). Induction of Apple Cucumber (Cucumis melo) Buds Development by Combination of Kinetin and IAA In B5 (Gamborg) Medium. Mangifera Edu, 6(1), 44–55. https://doi.org/10.31943/mangiferaedu.v6i1.114

Pardede Y et al. (2021). Pengaruh Hormon terhadap Induksi Embrio Somatik Kacapiring (Gardenia jasminoides) dan Potensi Aplikasinya dalam Pembuatan Benih Sintetik. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 162–177. https://doi.org/10.24002/biota.v6i3.4093

Pitaloka, D. (2017). Hortikultura: Potensi Pengembangan dan Tantangan. Jurnal article G-Tech, 1(1). https://dx.doi.org/10.33379/gtech.v1i1.260

Piter, O. Y., Ziraluo, B., Duha, M. (2020). Diversity Study Of Fruit Producer Plant In Nias Island. Jurnal Inovasi Penelitian, 1(4).

Ramdan, R., Handaji, N., Beyahia, H., dan Ibriz, M. (2014). Influence of Growth Regulators on Callus Induction from Embryos of Five Citrus Rootstocks. Journal of Applied Biosciences, 73, 5959–5965.

Rosita, A., Sugiono, D., dan Azizah, E. (2022). Invigorasi Benih Timun Apel (Cucumis melo L.) Dengan Kombinasi Zat Pengatur Tumbuh NAA (Naphtaleine Acetic Acid) dan Ekstrak Tauge Selama Periode Pembibitan. Jurnal Ilmiah Wahana Pendidikan, 8(10), 64-72.

Sapsuha, Y., Soetrisno, D., dan Kustantinah, K. (2011). Induksi Kalus Dan Embriogenesis Somatik In Vitro Pada Lamtoro (Leucaena Leucocephala). Berita Biologi, 10(5), 61804.

Saputro, N. W., Hidayat, T., Bayfurqon, F. M., dan Khamid, M. B. R. (2020). Evaluation of Morpho-agronomic Characterization Apple Cucumber: A new Variety of Melon from Indonesia. IOP Conference Series: Earth and Environmental Science, 457(1). https://doi.org/10.1088/1755-1315/457/1/012061

Sari, D. A., Slameto, dan Restanto, D. P. (2014). Induksi Tunas Kentang (Solanum tuberosum L.) Menggunakan BAP (Benzil Amino Purine). Berkala Ilmiah Pertanian, 1(1), 1-4

Sutter E.G., (1996). General Laboratory Recquiremets, Media and Sterelization Metods. In: Plant Tissue Culture Consept. New York: Laboratory Exercixes. CRC. Press Inc.

Toharah, N.I., S.D.J. Dwi., dan Z. Lalu. (2015). Pertumbuhan Kalus Daun Melon (Cucumis melo) Varietas MAI 119 dengan Pemberian BAP (Benzyl Amino Purine) dan 2.4 D (2.4 Dichlorophrmoxyacetic Acid). Jurnal Penelitian Pedidikan IPA, 1(2), 39-47.

Wulandari, S., Nisa, Y. S., Taryono, T., Indarti, S., dan Sayekti, R. S. (2021). Sterilisasi Peralatan dan Media Kultur Jaringan. Agrotechnology Innovation (Agrinova), 4(2), 16-19.

Ziraluo. (2021). Metode Perbanyakan Ubi Jalar Ungu (Ipomea batatas poiret) Dengan Teknik Kultur Jaringan Atau Stek Planlet. Jurnal Inovasi Penelitian. 2(3). https://doi.org/10.47492/jip.v2i3.819




DOI: https://doi.org/10.36987/agroplasma.v12i1.6550

Refbacks

  • There are currently no refbacks.


akun pro jepanghttps://disnakbun.rokanhulukab.go.id/togelsini/macaukece/buku mimpipaito hkjuaraslot

Jurnal Agroplasma

Program Studi Agroteknologi
Fakultas Sains dan Teknologi Universitas Labuhanbatu
JL. SM. Raja No. 126-A Km. 3,5 Aek Tapa Telp./Fax. (0624) 21901 Rantauprapat Kab. Labuhanbatu Sumatera Utara Pos. 21415
Email : agroplasma@ulb.ac.id

Creative Commons License

All publications by Jurnal Agroplasma [p-ISSN: 2303-2944] [E-ISSN:2715-033X] is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.