Identification, Pathogenesis and Virulence Test of Fungus Causes Postharvest Disease of Gedong Gincu Mango from Pal Market, Cimanggis, Depok

Inti Mulyo Arti, Paranita Asnur, Ratih Kurniasih, Evan Purnama Ramdan

Abstract


Mango is a climacteric fruit that can increase in maturity after harvest. As a result, mangoes will rot more easily because they are infected with pathogens such as fungi. The objectives of the study were to identify, examine the pathogenicity, and virulence of the fungus that causes postharvest disease in mangoes. Sampling of mangoes was carried out at the Pal market, Cimanggis, Depok by selecting fruits that were indicated to be infected with post-harvest diseases. Symptoms that appear are then described visually. Isolation of the fungus was carried out from the part of the mango fruit on Potato Dextrose Agar (PDA) media, then it was identified morphologically both macroscopically and microscopically. The virulence test was carried out by inoculating the fungus at 4 inoculation points of mango fruit. The results showed that the symptoms that appeared were symptoms of anthracnose in the form of blackish brown, sunken, and widespread spots on the mango skin. The results of morphological identification showed that the associated fungus was Colletotrichum gloeosporiodes which was characterized by white fungal colonies and fast growth, while the condia were cylindrical in shape with rounded ends. Confirmation results with Koch's postulates showed that postharvest anthracnose in mangoes was caused by C. gloeosporiodes. Meanwhile, virulence testing showed that C. gloeosporiodes had moderate to high virulence levels with symptomatic lesions ranging from 2,50 to 5,85 cm.


Keywords


Antrachnose; Colletotrichum gloeosporiodes; Postulat Koch.

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References


Ansari, A., Khanzada, M., Rajput, M., Maitio, S., Rajput, A., & Ujjan, A. (2018). Effect of Different Abiotic Factors on The Growth and Sporulation of Colletotrichum gloeosporioides Causing Antrhracnose of Mango. Plant Protection 02 (01), 23-30.

Archana, S., Prabakar, K., & Raguchander, T. (2014). Virulence Variation of Colletotrichum gloeosporioides (Penz.) Penz. and Evaluation of Varietal Susceptibility against Mango Anthracnose. Trends in Biosciences 7 (3), 415-421.

Arti, I. M., & Manurung, A. H. (2018). Pengaruh Etilen Apel dan Daun Mangga pada Pematangan Buah Pisang Kepok (Musa paradisiaca formatypica). Jurnal Pertanian Presisi, 2(2), 77-88.

Awa, O. C., Samuel, O., Oworu, O. O., & Sosanya, O. (2012). First Report of Fruit ANthracnose in Mango caused by Colletotrichum gloeosporioides in Southwestern Nigeria. International Journal of scientific & Technology Reasearch 1 (4), 30-34.

Badan Pusat Statistik. (2020). Produksi Tanaman Buah-buahan 2020. Jakarta: bps.go.id.

Benatar, G., Wibowo, A., & Suryanti. (2021). Karakterisasi dan Identifikasi Colletotrichum spp Penyeban Antraknosa Mangga di Indramayu. Yogyakarta: Universitas Gadjah Mada.

Chowdhury, M., & Rahim, M. (2009). Integrated crop management to control anthracnose (Colletotrichum gloesporioides) of mango. Journal of Agriculture and Rural Development 7.1 (2), 115-120.

Danh, Danh, L. T., Giao, B. T., Duong, C. T., Nga, N. T., Tien, D., . . . Trang, T. X. (2021). Use of Essential Oils for the Control of Anthracnose Disease Caused by Colletotrichum acutatum on Post-Harvest Mangoes of Cat Hoa Loc Variety. J. Membranes, 11(719), 1-18.

Darge, W., Woldemariam, S., & Niguisie, M. (2016). Survey of anthracnose (Colletotrichum gloeosporioides) on Mango (Mangifera indica) in North West Ethiopia. Plant Pathology & Quarantine 6 (2), 181-189.

Dhiedhiou, P., Mbaye, N., Drame, A., & Samb, P. (2007). Alteration of post harvest diseases of mango Mangifera indica through production practices and climatic factors. Journal of Biotechnology 6 (9), 1087-1094.

Gardjito, M., & Saifudin, U. (2011). Penanganan Pascapanen Buah-buahan Tropis. Yogyakarta: Kanisius.

Gautam, A. (2014). Colletotrichum gloeosporioides : Biology, Pathogenicity and Management in India. J. Plant Physiology Pathology 2(2), 1-11.

Harahap, T., Lubis, L., & Hasanuddin. (2013). Efek Temperatur Terhadap Virulensi Jamur Colletotrichum gloeosporioides Penz. Sacc. Penyebab Penyakit Antraknosa pada Tanaman Kakao (Theobroma cacao L.). Jurnal Agroekoteknologi, 411-420.

Jenny, F., Sultana, N., Islam, M., Khandaker, M. M., & Bhuiyan, M. A. (2019). A Review On Anthracnose of Mango Caused by Colletotrichum Gloeosporioides. Bangladesh J. Plant Phytopathological, 35(1 & 2), 65-74.

Khan, M., Chonhenchob, V., Huang, C., & Suwanamomlert, P. (2021). Antifungal Activity of Propyl Disulfide from Neem (Azadirachta indica) in VApor and Agar Diffusion Assays againts Anthracnose Pathogens (Colletotrichum gloeosporioides and Colletotrichum acutatum) in Mango Fruit. Microorganisms 9 (839), 1-13.

Konsue, W., Dethoup, T., & Limtong, S. (2020). Biological Control of Fruit Rot and Anthracnose of Postharvest Mango by Antagonistic Yeasts from Economic Crops Leaves. Microorganisms 8 (317), 1-16.

Mulyaningtyas, D., Purwantisari, S., Kusdiyantini, E., & Suryadi, Y. (2016). Produksi Kitosan Secara Enzimatik oleh Bacillus firmus E65 untuk Pengendalian Penyakit Antraknosa pada Buah Mangga (Mangifera indica L.). Jurnal Biologi 5 (4), 8-17.

Murdijati, G., & Saifudin, U. (2011). Penanganan Pascapanen Buah-buahan Tropis. Yogyakarta: Kanisius.

Nelson, S. C. (2008). Mango Anthracnose (Colletotrichum gloeosporioides). Plant Disease UH-CTAHR 48, 1-9.

Pelaez-Alvarez, A., Santos-Villalobos, S., Yepez, E., Parra-Cota, F., & Reyes-Rodriguez, R. (2016). Synergistic effect of Trichoderma asperelleum T8A and captan 50 against Colletotrichum gloeosporioides (Penz.). Rev. Mex. Cienc. AGric 7 (6), 1-4.

Ramdan, E. P., Arti, I. M., & Risnawati. (2019). Identifikasi dan Uji Virulensi Penyakit Antraknosa Pada Pascapanen Buah Cabai. Jurnal Pertanian Presisi, 3(1), 67-76.

Srideepthi, R., Lakshmisahitya, U., Peddakasim, D., Suneetha, P., & Krishna, M. (2017). Morphological, Pathological and Molecular Diversity of Colletotrichum Capsici inciting Fruit Rot in Chilli (Capsicum annuum L.). Res. J. Biotech, 14-21.

Supriatna, A., & Sudana, W. (n.d.).

Supriatna, A., & Sudana, W. (2008). Analisis Usahatani Mangga Gedong (Mangifera indica spp) (Studi Kasus di Kabupaten Cirebon, Jawa Barat). Jurnal Pengkajian dan Pengembangan Teknologi Pertanian 11 (3), 218-229.

Sutopo, A., Poerwanto, R., & Wiyono, S. (2017). Keefektifan Bahan Pencuci dan Pencegah Penyakit Terhadap Kualitas Buah Mangga cv. Gedong Gincu dan Arumanis. J. Hotikultura, 27(2), 253-260.

Torres-Calzada, C., Tapia-Tussell, R., Higuera-Ciapara, I., & Perez-Brito, D. (2012). MOrphological, Pathological and genetic diversity of Colletotrichum spesies Responsible for Anthracnose in papaya (Carica papaya L). Eur J. Plant Pathology, 1-13.

Tovar-Pedraza, J. M., Mora-Aguilera, J., Nava-Diaz, C., Lima, N., Michereff, S., Sandoval-Islas, J., Leyva-Mir, S. (2020). Distribution and Pathogenicity of Colletotrichum Spesies Associated With Mango Anthracnose in Mexico. J. Plant Disease, 104(1), 137-146.

Widiastuti, A., Ningtyas, O., & Priyatmojo, A. (2015). Identifikasi Cendawan Penyebab Penyakit Pascapanen pada Beberapa Buah di Yogyakarta. Jurnal Topatologi Indonesia, 91-96.

Widjanarko, S. B. (2012). Fisiologi dan Teknologi Pasca Panen. Malang: UB Press.

Widodo, & Hidayat, S. (2018). Identification of Colletotrichum species Associated with Chili Anthracnose in Indonesia by Morphological Characteristics and Spesies-Specific Primers. Asian Journal of Plant Pathology, 1819-1541.

Zivkovic, S., Stojanovic, S., Ivanovic, Z., Trkulja, N., Dolovac, N., Aleksic, G., & Balaz, J. (2010). Morphological and Molecular Identification of Colletotrichum acutatum from Tomato Fruit. Pestic. Phytomed. 25 (3), 231-239.




DOI: https://doi.org/10.36987/jpbn.v8i2.2628

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