Antagonistic and Antibacterial Activity of Stapylocossus aureus and Isolates of Oral Bacteria from the Endogenous Fungus Apis dorsata Binghami Nest

Mokosuli Yermia Semuel, Masje Wurarah, Reinny Silvana Tuegeh

Abstract


Apis Dorsata Binghami honey bee is a honey bee endemic to Indonesia, living naturally in the forests of Sulawesi. This study aimed to obtain isolates and characteristics of endogenous fungi antibiotic activity from Apis dorsata Binghami nest. The research consisted of isolation of fungi from beehives using potato dextro agar, pure culture of fungi, antagonist test and antibiotic test using disc diffusion method. Antibiotic test was performed on oral bacteria and Staphylococcus aureus. The results obtained six isolates of fungi from the nest of Apis dorsata Binghami, namely isolates FAB1, FAB2, FAB3, FAB4, FAB5 and FAB6. The results of the antagonist test showed that the isolates FAB2 and FAB3 had the best antagonist properties, while the FAB6 isolates had the weakest antagonist properties. The FAB2 isolate showed the best bacterial growth inhibition zone average for the isolates of oral bacteria and Staphylococcus aureus. The inhibition zone lasts up to 3 x 24 hours so that the activity of the bacteria is bactericidal. From the results of this study, it can be concluded that the endogenous fungus Apis dorsata Binghami is a potential source of antibacterial bioactives.


Keywords


Nest; Apis dorsata Binghami; Fungi; Endogenous; Antibacterial; Antagonistic

Full Text:

PDF

References


AL-Ani, I., Zimmermann, S., Reichling, J., & Wink, M. (2018). Antimicrobial Activities of European Propolis Collected from Various Geographic Origins Alone and in Combination with Antibiotics. Medicines, 5(1). https://doi.org/10.3390/medicines5010002

Aziz, L., Iftikhar, K., Kamdar, H., & Khalid, S. (2020). Significance of Soil Fungi as an Inexaustible Source of Antibiotics. 1(I).

Bhagat, J., Kaur, A., Kaur, R., Yadav, A. K., Sharma, V., & Chadha, B. S. (2016). Cholinesterase inhibitor (Altenuene) from an endophytic fungus Alternaria alternata: optimization, purification and characterization. Journal of Applied Microbiology, 121(4), 1015–1025. https://doi.org/10.1111/jam.13192

Bozoudi, D., & Tsaltas, D. (2018). The multiple and versatile roles of Aureobasidium pullulans in the vitivinicultural sector. Fermentation, 4(4), 1–15. https://doi.org/10.3390/fermentation4040085

Galeotti, F., Maccari, F., Fachini, A., & Volpi, N. (2018). Chemical composition and antioxidant activity of propolis prepared in different forms and in different solvents useful for finished products. Foods, 7(3). https://doi.org/10.3390/foods7030041

Gao, Y., Stuhldreier, F., Schmitt, L., Wesselborg, S., Guo, Z., Zou, K., Mándi, A., Kurtán, T., Liu, Z., & Proksch, P. (2020). Induction of New Lactam Derivatives From the Endophytic Fungus Aplosporella javeedii Through an OSMAC Approach. Frontiers in Microbiology, 11(November), 1–13. https://doi.org/10.3389/fmicb.2020.600983

Hadisoesilo, S. (2001). Keanekaragaman Spesies Lebah Madu Asli Indonesia. Biodiversitas, 2(1), 123–128.

Hepburn, H. R., & Radloff, S. E. (2011). Honeybees of Asia. Honeybees of Asia, 1–669. https://doi.org/10.1007/978-3-642-16422-4

Izzatinnisa, Utami, U., & Mujahidin, A. (2020). Uji Antagonisme Beberapa Fungi Endofit pada Tanaman Kentang terhadap Fusarium oxysporum secara In Vitro Antagonistic Effect of Several Endophyte Fungi in Potato Plants against Fusarium oxysporum In Vitro. Jurnal Riset Biologi Dan Aplikasinya, 2(1), 18–25. https://journal.unesa.ac.id/index.php/risetbiologi

Kanan, M., Salaki, C., & Mokosuli, Y. S. (2020). Molecular identification of bacterial species from musca domestica L. And Chrysomya megachepala L. And luwuk City, Central sulawesi, indonesia. Journal of Pure and Applied Microbiology, 14(2), 1595–1607. https://doi.org/10.22207/JPAM.14.2.58

Leathers, T. D., Rich, J. O., Bischoff, K. M., Skory, C. D., & Nunnally, M. S. (2019). Inhibition of Streptococcus mutans and S. sobrinus biofilms by liamocins from Aureobasidium pullulans. Biotechnology Reports, 21, e00300. https://doi.org/10.1016/j.btre.2018.e00300

Mokosuli, Y. S. (2013). Karakter Morfologi, Sumber Pakan dan Bioaktivitas Farmakologis Racun Lebah Madu Endemik Sulawesi Apis dorsata Binghami dan Apis nigrocincta Smith (Hymenoptera: Apidae).[Disertasi]. Program Pascasarjana Universitas Sam Ratulangi Manado.

Mursalim, M. F., & Jamaluddin, A. W. (2019). AKTIVITAS ANTIMIKROBA KOMBINASI EKSTRAK PROPOLIS Trigona sp DAN JAHE (Zingiber officinale ROSCOE) TERHADAP BAKTERI Salmonella thypimurium. Jurnal Ilmiah As-Syifaa, 11(1), 70–74. https://doi.org/10.33096/jifa.v11i1.471

Nagir, M. T. (2016). The distribution and nest-site preference of Apis dorsata binghami at Maros Forest , South Sulawesi , Indonesia. 4(23), 1–14.

Prestianti, I. K. A., Sains, F., & Teknologi, D. A. N. (2017). Universitas islam negeri alauddin makassar 2017.

Przybyłek, I., & Karpiński, T. M. (2019). Antibacterial properties of propolis. Molecules, 24(11), 11–13. https://doi.org/10.3390/molecules24112047

Rosalina, R., Ningrum, R. S., & Lukis, P. A. (2018). Aktifitas Antibakteri Ekstrak Jamur Endofit Mangga Podang ( Mangifera indica L .) Asal Kabupaten Kediri Jawa Timur. Majalah Ilmiah Biologi Biosfera: A Scientific Journal, 35(3), 139–144. https://doi.org/10.20884/1.mib.2018.35.3.757

Semuel, M. Y., Kaunang, E. S. N., & Manoppo, J. S. S. (2019). Potensi Bioaktif dari Apis dorsata Binghami , Lebah Madu endemik Sulawesi. 98.

Semuel, M. Y., Repi, R. A., Worang, R. L., Mokosuli, C., & Semuel, Y. (2017). Potential antioxidant and anticancer effect of Apis dorsata Binghami Crude Venom from Minahasa, North Sulawesi. Journal of Entomology and Zoology Studies JEZS, 112(52), 112–119.

Semuel, M. Y., Rudi, D., & Repi, A. (2015). The Characteristics Of Bioactive Peptides And Antibacterial Activity Of Honey Bee (Apis nigrocincta) Smith Venom, Endemic To Sulawesi. Molekul, 10(2), 135–144. https://ojs.jmolekul.com/ojs/index.php/jm/article/view/4

Semuel, M. Y., Sherly, E., Kaunang, N., Manopo, J. S., Tondano, K. U., Utara, S., Mathematics, F., Science, N., Tondano, U., Utara, S., Mathematics, F., Science, N., Tondano, K. U., & Utara, S. (2019). The bioactive contents and antioxidant activity of honey bee nest extract of Apis dorsata Binghami from the North Sulawesi. Molekul, 14(2), 92–102. https://doi.org/Articles https://doi.org/10.20884/1.jm.2019.14.2.502

Viuda-Martos, M., Ruiz-Navajas, Y., Fernández-López, J., & Pérez-Ãlvarez, J. A. (2008). Functional properties of honey, propolis, and royal jelly. Journal of Food Science, 73(9), 117–124. https://doi.org/10.1111/j.1750-3841.2008.00966.x




DOI: https://doi.org/10.36987/jpbn.v8i2.2739

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Mokosuli Yermia Semuel, Masje Wurarah, Reinny Silvana Tuegeh

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

 






Jurnal Pembelajaran dan Biologi Nukleus (JPBN) by LPPM Universitas Labuhanbatu is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (CC BY - NC - SA 4.0)