Antimicrobial Activity of Endophyte Bacteria Isolated from Seaweed (Padina sp.) on the Growth of Bacteria Escherichia coli and Staphylococcus aureus

Fanesa Akay, Jantje Ngangi, Helen J Lawalata

Abstract


Padina sp. seaweed is one of the Chlorophyta which has potential as an antimicrobial. This study aimed to determine the antimicrobial activity of Padina sp. seaweed endophytic bacteria against Escherichia coli and Staphylococcus aureus. The method used in this research is descriptive qualitative. The results of the isolation of endophytic microbes of Padina sp. seaweed obtained were seven isolates DP1, DP2, DP3, DP4, AP1, AP2, and AP3. The results of the antimicrobial activity test showed that the seven isolates of endophytic bacteria had potential as antimicrobials. Incubation time for 24 hours showed that the diameter of the inhibition zone formed was 9.75 – 11.55 mm against S. aureus and 7.15 – 10.0 mm against bacteria. E. coli. The AP3 and DP1 isolates had a wider zone, presumably because their antibacterial substances were higher than the other isolates.


Keywords


Antimicrobial, Seaweed, Padina sp

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References


Aliah, N., Susilawaty, A., & Ibrahim, I. (2015). Uji Efektivitas Ekstrak Daun Cengkeh (Syzigium aromaticum) Sebagai Repellent Semprot Terhadap Lalat Rumah (Musca Domestica). Vol 2(3), 113-120.

Alviana, N., 2016, Uji Efektivitas Antibakteri Ekstrak Etanol Daun Krisan (Chrysanthemum morifolium Syn. Dendrathema grandiflora) Terhadap Staphylococcus aureus dan Escherichia coli, Skripsi, Fakultas Teknobiologi Universitas Atma Jaya, Yogyakarta.

Arlita.2013. Identifikasi pigmen karotenoid pada bakteri Simbion rumput laut Caulerpa cupressoides (Vahl) C. Agardh. Journal Of Marine Research.

Aziza. 2018. Potensi Antagonis Bakteri Toleran Salin Terhadap Ralstonia Solanacearum Secara In Vitro Dan Pengaruhnya Terhadap Perkecambahan Benih Tomat Dibawah Cekaman Salin. Universitas Brawijaya

Brooks, G.F., Janet, S.B., Stephen A.M. 2005. Jawetz, Melnick and Adelbergs,. Mikrobiologi Kedokteran (Medical Microbiology) Buku I, Alih Bahasa.

Dzeha T, Jaspars M, Jioji T. 2003. Clionasterol, a triterpenoid from the Kenyan marine green macroalga Halimeda macroloba. Western Indian Ocean Journal of Marine Science. 2(2): 157-161.

Eka, Sari. 2018. Isolasi dan Karakterisasi dari Tanaman Kedelai dan Tanaman Putri Malu. Bangka. : Universitas Bangka Belitung.

Hollants J, Leliaert F, De Clerck O, Willems A (2011) How endo- is endo-? Surface sterilization of delicate samples: a Bryopsis (Bryopsidales, Chlorophyta) case study. Symbiosis 51: 131–138.

Janakidevi et al. 2013. The antimicrobial effects of the Ae-ZnONPs against totally eight bacterial strains and two fungal strains were used throughout the investigation. The bacteria used were Bacillus subtilis, Staphylococcus aureus, E. coli, Pseudomonas aerogenosa, Enterococcus faecalis, Micrococcus luteus, Shigella fluxneri, and Serratia marcescens. The fungal strains used were Aspergillus niger and Candida albicans.

Jawetz, Melnick., Adelberg. 2008. Mikrobiologi kedokteran. Edisi ke – 23. Jakarta :ECG

Karimela, E.J., Frans.G.I., Henny.A.D. 2017. Karakteristik Staphylococcus aureus yang Diisolasi dari Ikan Asap Pinekuhe Hasil Olahan Tradisional Kabupaten Sangihe. http://journal.ipb.ac.id/. Diakses pada tanggal 30 Januari 2022.

Melliawati, R. 2009. E. coli dalam kehidupan manusia. Biotrends/Vol.4/No.1/Th.2009.

Meng J dan Schroeder CM. 2007. Escherichia coli. Ch 1 In: Simjee S. (ed) Foodborne diseases. Totowa: Humana Press. Pp. 1-25.

Prihatiningtyas W. 2006. Mikroba Endofit, Sumber Penghasil Antibiotik yang Potensial. Yogyakarta: Fakultas Farmasi UGM.

Rahmi Y, Darmawi, Mahdi A, Faisal J, Fakhrurrazi, dan Yudha F. 2015. Identification of Staphylococcus aureus in preputium and vagina of horses (Equus caballus). Journal Medika Veterinaria. 9(2): 15-158.

Retri. 2021. Isolasi Bertahap Bakteri Pendegradasi Limbah Tandan Kosong Kelapa Sawit di PT. Erasakti Wira Forestama Muaro Jambi. Universitas Jambi

Rosana, Dadan. (2014). Modul Kuliah Biofisika. Yogyakarta: FMIPA UNY

Rozirwan et al. 2015. Senyawa bioaktif bakteri simbion pada karang lunak Sinularia flexibiliS dan S. polydactyla.

Siregar. 2008. Pengaruh Vitamin C terhadap Peningkatan. Hemoglobin (Hb) Darah dan Kelulushidupan Benih Ikan Kerapu Bebek(Cromileptes altivelis). Jurnal Natur Indonesia 12

(1) :75-81.

Soedarto. (2015). Mikrobiologi Kedokteran . jakarta: CV. Sagung Seto.

Strobel, G. and Daisy, B. (2003) Bioprospecting for Microbial Endophytes and Their Natural Products. Microbiology and Molecular Biology Reviews, 67, 491-502.

Susanto. 2002. Isolasi Dan Identifikasi Bakteri Proteolitiki Terhadap Deproteinasi Limbah Cangkang Rajungan Pada Proses Pembuatan Kitin, Jurnal Sains Dan Teknologi BPPT, 4 : 9-14.

Syahrurahman, A., et al. 2010. Buku Ajar Mikrobiologi Kedokteran. Edisi Revisi. Binarupa Aksara Publisher.Jakarta.

Lawalata, Helen J.,Rengkuan Mariana, Satiman Utari. 2019. “Antibacterial Activities of lactic acid bacteria from langsat fruit (lansiumdomesticum) against phatogenic bacteria and spoilage bacteriaâ€. Internationa Journal of Environment, Agriculture and Blotechnology (IJAEB).

Lawalata, Helen J., L. Sembiring, and E. S. Rahayu. 2010. “Bakteri asam laktat pada bakasang dan aktivitas penghambatnya terhadap bakteri patogen dan pembusuk. “Prosiding Seminar Nasional Biologi. Yogyakarta: Fakultas Biologi UGM




DOI: https://doi.org/10.36987/jpbn.v9i2.3632

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