Tolerance of Melon (Cucumis melo L.) Cultivar Melodi Gama 3 to Powdery Mildew Infection

Rozikin Rozikin, Budi Setiadi Daryono

Abstract


Powdery mildew is one of melon disease caused by ascomycetes fungus from genus Sphaerotheca and Erysiphe. Breeding is considered to be the best approach to control Powdery mildew infection especially in melon. MG3 is a melon cultivar derived from ‘MG1’ x ‘Ladika’ and its estimate carry out resistant gene to Powdery mildew infection inherited from MG1. This research is to find out percentage and phenotype characters of Powdery mildew infection in MG3 compared to four commercial melons (Action 434, Glamour, MAI and ladika) that are popular in the market. Five melons are planted in greenhouse B of KP4 UGM. After five weeks, the plants where inoculated with Powdery mildew KP4 UGM in leaves. Scoring in three days after inoculation and the percentage of infections measured every 3 days for eight times using grid line 21 cm x 21 cm with small cubics 1,5 cm x 1,5 cm. The analysis of infections level in leaves, plant and population was done using Fukino protocol. The results show that cultivar MG3 tolerant and stable to Powdery mildew infection compare to cultivar Action 434, MAI and ladika in leaves, plant and population level. The infection of Powdery mildew caused damage to the leaves tissue, decreasing the abilities of photosynthesis resulting in necrotic, chlorotic and dead of the infected leaves. In fruits, infection causes break in the net, immature and rotten flesh, decrease sweetness and scent in cultivar Action434, MAI and Ladika. Base on microscopic analysis, Powdery mildew isolat KP4 UGM is a member of from genus Podosphaera.

Keywords


Breeding, Melon, MG3, Powdery mildew

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References


Cao, Y., Diao, Q., Chen, Y., Jin, H., Zhang, Y., & Zhang, H. (2021). Development of KASP Markers and Identification of a QTL Underlying Powdery Mildew Resistance in Melon (Cucumis melo L.) by Bulked Segregant Analysis and RNA-Seq. Frontiers in Plant Science, 11. https://doi.org/10.3389/fpls.2020.593207

Cui, H., Fan, C., Ding, Z., Wang, X., Tang, L., Bi, Y., Luan, F., & Gao, P. (2022). CmPMRl and CmPMrs are responsible for resistance to powdery mildew caused by Podosphaera xanthii race 1 in Melon. Theoretical and Applied Genetics, 135(4). https://doi.org/10.1007/s00122-021-04025-4

Cui, L., Siskos, L., Wang, C., Schouten, H. J., Visser, R. G. F., & Bai, Y. (2022). Breeding melon (Cucumis melo) with resistance to powdery mildew and downy mildew. Horticultural Plant Journal, 8(5), 545–561. https://doi.org/10.1016/j.hpj.2022.07.006

Daryono, B. S., Aristya, G. R., & Kasiamdari, R. S. (2011). Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon. Daryono et Al. Indonesian Journal of Biotechnology, 16(2), 76–82. https://doi.org/10.4308/hjb.16.4.142

Daryono, B. S., & Fitriyah Fauziatul. (2016). PEWARISAN KETAHANAN MELON (Cucumis melo L.) KULTIVAR MELODI GAMA 3 TERHADAP. Jurnal Perlindungan Tanaman Indonesia, 20(2), 59–64.

Fukino, N., Kunihisa, M., & Matsumoto, S. (2004). Characterization of recombinant inbred lines derived from crosses in melon (Cucumis melo L.), “PMAR No. 5†x “Harukei No.3.†Breeding Science, 54(2), 141–145. https://doi.org/10.1270/jsbbs.54.141

Garcia-Mas, J., Benjak, A., Sanseverino, W., Bourgeois, M., Mir, G., Gonźalez, V. M., Heńaff, E., Cam̂ara, F., Cozzuto, L., Lowy, E., Alioto, T., Capella-Gutieŕrez, S., Blancae, J., Cañizares, J., Ziarsolo, P., Gonzalez-Ibeas, D., Rodriǵuez-Moreno, L., Droege, M., Du, L., … Puigdomeǹech, P. (2012). The genome of melon (Cucumis melo L.). Proceedings of the National Academy of Sciences of the United States of America, 109(29), 11872–11877. https://doi.org/10.1073/pnas.1205415109

Ivanova, Z., Vasileva, K., Velkov, N., & Grozeva, S. (2018). Evaluation of powdery mildew resistance in various melon (Cucumis melo L.) genotypes. Agricultural Science and Technology, 10(4), 279–284. https://doi.org/10.15547/10.15547/ast.2018.04.053

Natarajan, S., Kim, H. T., Thamilarasan, S. K., Veerappan, K., Park, J. I., & Nou, I. S. (2016). Whole genome re-sequencing and characterization of powdery mildew disease-associated allelic variation in melon. PLoS ONE, 11(6), 1–19. https://doi.org/10.1371/journal.pone.0157524

Wang, S., Yan, W., Yang, X., Zhang, J., & Shi, Q. (2021). Comparative methylome reveals regulatory roles of DNA methylation in melon resistance to Podosphaera xanthii. Plant Science, 309. https://doi.org/10.1016/j.plantsci.2021.110954




DOI: https://doi.org/10.36987/jpbn.v9i1.3815

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