The Effect of IAA and BAP on Root Induction of Cattleya Orchids

Fauziyah Harahap, Kenari Br Sinuraya, Syarifuddin Syarifuddin, Cicik Suriani, Ayu Putri Ningsih, Syahmi Edi, Nusyirwan Nusyirwan

Abstract


Orchid is an ornamental plant commodity that has an important meaning in international trade. Until now, orchids are still the center of attention of farmers and lovers of ornamental plants, because of their bright potential as cut flowers and potted plants. The popular and widespread type of orchid is Cattleya sp. The Cattleya orchid is also called the queen of orchids because of its colorful flowers. Cattleya sp. orchids take a long time to propagate by seed, about 4 to 7 years, so other methods are needed to deal with them. Tissue culture is a method for isolating plant parts such as cells, tissues or organs, and cultivating them in an aseptic environment. The purpose of this study was to determine the optimal interaction of growth regulators indole acetic acid (IAA), benzyl amino purine (BAP), and their interactions with the root induction of Cattleya sp. Cytokinins interact with auxin in determining the direction of cell differentiation. In this research method, differences in concentrations of indole acetic acid (IAA: 0, 2, 4, 6 ppm) and concentrations of benzyl amino purine (BAP: 0, 0, 5, 1 ppm) were carried out. Parameters observed were the number of roots and root length. Data were analyzed descriptively and inferentially using ANOVA. The highest number of roots was obtained in the treatment medium containing 0.5 ppm BAP and 6 ppm IAA, which was 6.33. The highest root length was found in the B0I4 treatment (BAP 0 + IAA 4 ppm). The results of the analysis of the interaction variance between the IAA treatment and the BAP treatment were 0.005, meaning that there was a significant effect of the combination of IAA and BAP on the leaf length of Cattleya sp.

Keywords


Orchid Cattleya sp., In Vitro, Growth regulator

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References


Basry, Arie. 2016. Study of the Utilization of Tissue Culture in Virus-Free Plant Propagation. bppsdmp.pertanian.go.id : 64-73.

Central Bureau of Statistics and Directorate General of Horticulture. 2014. Orchid Production by Province. 2010-2014. Horticulture Subsector. Accessed from . http://www.pertanian.go.id/ap-pages/mod/datahorti .

Gunawan, LW 1987. Tissue culture techniques. Bogor. Plant Tissue Culture Laboratory

Harahap, F., Roedhy P., Suharsono, Cicik S. and Suci R. 2014. In Vitro Growth and Rooting of Mangosteen (Garcinia mangostana L.) on Medium With Various Concentrations of Growth Regulators. Journal of Biological Biosciences. Vols 21 (4): 151-158

Harahap, F. (2010) Practical Techniques to Make Orchids Always Flower. Community service. 16(61):41-50.

Harapan, F. (2011). Plant tissue isolation method. Medan : United Press.

Harahap. F., Poerwanto. R., Spbir., Hasruddin., Suriani. C., Siallagan, J & Rohyan. (2015). Sterilization of Pineapple Explan from Sipahutar, North Sumatra, Indonesia (Ananas comosus L) and In vitro Growth Induction. Asian Jr. of Microbal. Biotect. Env.Sc. 17 (2) : 469-478

Henuhili V. 2013. Plant Tissue Culture. Yogyakarta: UNY Press.

Isda, MN, and Fatonah, S. (2014). Root induction in Orchid shoot explants

Grammatophylum scriptum var. In Vitro Oranges on MS Media with the Addition of NAA and BAP. Journal of Biology Al Kauniyah. 7(2): 53-57

Iswanto . (2007). Orchid care instructions. Jakarta: Agromedia Pustaka .

Karjadi, AK & Buchory, C. (2007). Effect of NAA and BAP on Garlic Meristem Tissue Growth in B5 Media. J. Hort , 17(3): 217-223

Lestari, EG (2011). The Role of Growth Regulatory Substances in Plant Propagation Through Tissue Culture. Journal of AgroBiogen, 7(1): 63-68

Miryeganeh, Matin, 2022. Ephigenetic Mechanism of Senescence in Plants. Cells. 11(2) : 251. doi.10.3390/cells11020251.

Nika, SL, Siregar, LAM, Kardhinata, EH, (2018). Success in the form of Orchid Microshoots (Cattleya trianae Lindl & Rchb.fil.) in several composition media. Journal of Agroecotechnology FPUSU, 6(1): 113-117.

Novak, Stacey. Lila J Luna. Roshan. 2014. Role of Auxin in Orchid Development. Plant Signaling & Behavior. 9(10).

Panjaitan, E. (2005). Growth Response of Orchid (Dendrobium sp.) to Administration of BAP and NAA In Vitro. Journal of Agricultural Science Research, 3(3): 45-51.

Paramartha, Aisya., Dini Ermavitalini, Siti Nurfadilah. (2012). The Effect of the Addition of NAA and BAP ZPT Concentrations on the Growth and Development of Dendrobium taurulinum J.J Smith Seeds in Vitro. Journal of Science and Arts ITS. 1(1) : E40-E43.

Sulistiani E, Samsul AY. 2015. Production of Plant Seeds Using Tissue Culture Techniques. Bogor: SEAMEO BIOTROP.

Thengane, SR, Deodhar, SR, Bhosle, SV & Rawal, SK 2006, 'Direct somatic embryogenesis and plant regeneration in Garcinia indica Choiss', Current Science, vol. 91 no. 8, pp 1074-8.

Widiastoety, D: Effect of Auxin and Cytokinin on the Growth of Mokara Orchid Plantlets (Effect of Auxin and Cytokinin on the Growth of Mokara Orchid Plantlets) J. Hort. 24(3):230-238, 2014




DOI: https://doi.org/10.36987/jpbn.v9i2.4481

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