In Vitro Antidiabetic Activity and Bioactive Ingredients of Apis mellifera and Apis dorsata Binghami Nest Extracts

Henike Sibala, Orbanus Naharia, Nonny Manampiring, Yermia Semuel Mokosuli

Abstract


Indonesia ranks 7th out of ten countries with diabetes mellitus, which continues to increase yearly. Honeycomb is rich in secondary metabolites formed from plant resins (propolis) which contain alkaloids, flavonoids, saponins, tannins, steroids and triterpenes. The research aimed to analyze the comparison of flavonoid content and antidiabetic activity with the enzyme α-glucosidase In Vitro nest extract of A. mellifera and A. dorsata Binghami. The samples used were nests of A. mellifera from northern Toraja, southern Sulawesi and A. dorsata Binghami from Southeast Minahasa, North Sulawesi. Using 96% ethanol, HPLC method of flavonoid testing and in vitro antidiabetic activity testing using α-glucosidase enzymes. The flavonoid content results were based on the number of peaks produced and the retention time of A. mellifera produced 26 compounds, and A. dorsata Bingham produced 21 compounds. The results of in vitro antidiabetic tests with α-glucoside enzyme inhibitors obtained IC50 values from the nest extract of A. mellifera of 3.605 mg/L and the nest extract of A. dorsata Binghami of 4.992 mg/L. The extract from A. mellifera had better antidiabetic potential than that of A. dorsata Binghami, although the difference is insignificant.


Keywords


antidiabetic; nest extract, Apis dorsata Binghami, Apis mellifera

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Asem, N., Abdul Gapar, N. A., Abd Hapit, N. H., & Omar, E. A. (2020). Correlation between total phenolic and flavonoid contents with antioxidant activity of Malaysian stingless bee propolis extract. Journal of Apicultural Research, 59(4), 437-442. https://doi.org/10.1080/00218839.2019.1684050

Awang, N., Ali, N., Majid, F. A. A., Hamzah, S., & Razak, S. B. A. (2018). Total flavonoids and phenolic contents of sticky and hard propolis from 10 species of Indo-Malayan stingless bees. Malaysian Journal of Analytical Sciences, 22(5), 877–884. https://doi.org/10.17576/mjas-2018-2205-15

Balica, G., Vostinaru, O., Stefanescu, C., Mogosan, C., Iaru, I., Cristina, A., & Pop, C. E. (2021). Potential role of propolis in the prevention and treatment of metabolic diseases. Plants, 10(5), 1– https://doi.org/10.3390/plants10050883

Cornara, L., Biagi, M., Xiao, J., & Burlando, B. (2017). Therapeutic properties of bioactive compounds from different honeybee products. Frontiers in Pharmacology, 8(JUN), 1–20. https://doi.org/10.3389/fphar.2017.00412

Da Cruz, F. B., Martins Nascimento, D. H., Martins, N., Ferreira, J. D. F., Silveira, D., & Yris Maria, B. F. (2022). Antioxidant Activity of Apis Mellifera Bee Propolis : A review. 1(1). https://doi.org/10.24377/jnpd.article655

De Lima, D., Lamerkabel, J. S. A., & Welerubun, I. (2020). Inventarisasi Jenis-Jenis Tanaman Penghasil Nektar Dan Polen Sebagai Pakan Lebah Madu Apis Mellifera Di Kecamatan Kairatu Kabupaten Seram Bagian Barat. Agrinimal Jurnal Ilmu Ternak Dan Tanaman, 7(2), 77–82. https://doi.org/10.30598/ajitt.2019.7.2.77-82

Dian Puspitasari, A., & Pramono, S. (2015). Comparison Of Methods Of Producing Bee Propolis Purified Extract Based On Total Flavonoid Content Using Rutin As Standard Perbandingan Metode Pembuatan Ekstrak Terpurifikasi Bee Propolis Dari Lebah Madu (Apis Mellifera) Berdasarkan Kadar Flavonoid Total D. Traditional Medicine Journal, 20(2), 2015.

Egra, S., Mardhiana, ., Rofin, M., Adiwena, M., Jannah, N., Kuspradini, H., & Mitsunaga, T. (2019). Aktivitas Antimikroba Ekstrak Bakau (Rhizophora mucronata) dalam Menghambat Pertumbuhan Ralstonia Solanacearum Penyebab Penyakit Layu. Agrovigor: Jurnal Agroekoteknologi, 12(1), 26. https://doi.org/10.21107/agrovigor.v12i1.5143

Hadisoesilo, S. (1995). Review: The Diversity of Indigenous Honey Bee Species of Indonesia. Biodiversitas Journal of Biological Diversity, 2(1), 123–128. https://doi.org/10.13057/biodiv/d020107

Handayani, T. H. (2022). Aktivitas Antioksidan, Total Fenolik, dan Total Flavonoid Madu Apis mellifera dari Hutan Akasia (Accacia crassicarpa) Riau, Indonesia dengan Beberapa Perlakuan Pengeringan. Jurnal Biologi Indonesia, 18(2), 231–243. https://doi.org/10.47349/jbi/18022022/231

Handoko, C., & Hidayatullah, M. (2017). Kajian Migrasi Lebah Hutan Sumbawa Di Kphp Batulanteh. Jurnal Penelitian Kehutanan Faloak, 3(2), 87–100. https://doi.org/10.20886/jpkf.2019.3.2.87-100

Hardoko, H., Febriani, A., & Sirantri, T. (2015). Invitro Antidiabetic Activities of Agar, Agarosa, and Agaropectin from Gracilaria gigas Seaweed. Jurnal Pengolahan Hasil Perikanan Indonesia, 18(2), 128–139. https://doi.org/10.17844/jphpi.2015.18.2.128

Harborne, G. C., Watson, F. L., Healy, D. T., & Groves, L. (1996). The effects of sub-anaesthetic doses of ketamine on memory, cognitive performance and subjective experience in healthy volunteers. Journal of Psychopharmacology, 10(2), 134-140. https://doi.org/10.1177/026988119601000208

Inhibisi, M., & Glukosidase, E. Α. (2021). Semnas MIPAKes Umri. 100–110.

Kara, Y., Can, Z., & Kolaylı, S. (2022). What Should Be the Ideal Solvent Percentage and Solvent-Propolis ratio in the Preparation of Ethanolic Propolis Extract? Food Analytical Methods, 15(6), 1707–1719. https://doi.org/10.1007/s12161-022-02244-z

Kemenkes. (2020). Infodatin tetap produktif, cegah, dan atasi Diabetes Melitus 2020. In Pusat Data dan Informasi Kementrian Kesehatan RI (pp. 1–10). https://pusdatin.kemkes.go.id/resources/download/pusdatin/infodatin/Infodatin-2020-Diabetes-Melitus.pdf

Kim, Y. M., Jeong, Y. K., Wang, M. H., Lee, W. Y., & Rhee, H. I. (2005). Inhibitory effect of pine extract on α-glucosidase activity and postprandial hyperglycemia. Nutrition, 21(6), 756–761. https://doi.org/10.1016/j.nut.2004.10.014

Kocot, J., Kiełczykowska, M., Luchowska-Kocot, D., Kurzepa, J., & Musik, I. (2018). Antioxidant potential of propolis, bee pollen, and royal jelly: Possible medical application. Oxidative Medicine and Cellular Longevity, 2018. https://doi.org/10.1155/2018/7074209

Lee, D. S., & Lee, S. H. (2001). Genistein, a soy isoflavone, is a potent α-glucosidase inhibitor. FEBS Letters, 501(1), 84–86. https://doi.org/10.1016/S0014-5793(01)02631-X

Mambiya, M., Shang, M., Wang, Y., Li, Q., Liu, S., & Yang, L. (2019). The Play of Genes and Non-genetic Factors on Type 2 Diabetes. 7(November), 1–8. https://doi.org/10.3389/fpubh.2019.00349

Matuszewska, E., Klupczynska, A., Maciołek, K., Kokot, Z. J., & Matysiak, J. (2021). Multielemental analysis of bee pollen, propolis, and royal jelly collected in west-central poland. Molecules, 26(9), 1–18. https://doi.org/10.3390/molecules26092415

Mayur, B., Sandesh, S., Shruti, S., & Sung-yum, S. (2010). Antioxidant and α-glucosidase inhibitory properties of.pdf. 4(15), 1547–1553. https://doi.org/10.5897/JMPR10.210

Mohammad, S. M., Mahmud-Ab-Rashid, N. K., & Zawawi, N. (2021). Stingless bee-collected pollen (bee bread): Chemical and microbiology properties and health benefits. Molecules, 26(4), 1–29. https://doi.org/10.3390/molecules26040957

Mokosuli, Y. S. (2021). Brine Shrimp Lethality Test (BSLT) Ekstrak Sarang Lebah Madu (Apis dorsata Binghami. Jurnal Pendidikan Biologi Undiksha, 8, 138–144. https://ejournal.undiksha.ac.id/index.php/JJPB/article/view/37506%0Ahttps://ejournal.undiksha.ac.id/index.php/JJPB/article/download/37506/19923

Mokosuli, Y. S., Kaunang, E. S. N., & Manoppo, J. S. S. (2019). Potensi Bioaktif dari Apis dorsata Binghami , Lebah Madu endemik Sulawesi. 98.

Mokosuli, Y., Sumampouw, H., Maliangkay, H. P., & Langi, J. (2021). The Bioactive Content And Antioxidant Activity Of Honey Beehives ( Apis Dorsata Binghami ) From The Bogani Nani Wartabone Bolaang Mongondow National Plant. 2(1), 18–29.

Mooy, B. Z. (2021). Kajian Pola Migrasi Lebah Hutan Timor (Apis Dorsata) Di Kawasan Cagar Alam Gunung Mutis-Timor Tengah Selatan, Provinsi Nusa Tenggara Timur. Jurnal Bestari, 2(1), 23–34. https://jurnalbestari.ntbprov.go.id/index.php/bestari1/article/view/35%0Ahttps://jurnalbestari.ntbprov.go.id/index.php/bestari1/article/download/35/19

Mustari, A. H. (2020). Persebaran Flora Dan Fauna Endemik Di Pulau Sulawesi. In Jurnal Lingkungan Hidup (Vol. 2, Issue 2).

Nowak, A., Szczuka, D., Górczyńska, A., Motyl, I., & Kręgiel, D. (2021). Characterization of apis mellifera gastrointestinal microbiota and lactic acid bacteria for honeybee protection—a review. Cells, 10(3), 1–29. https://doi.org/10.3390/cells10030701

Özkök, A., Keskin, M., Tanuğur Samancı, A. E., Yorulmaz Önder, E., & Takma, Ç. (2021). Determination of antioxidant activity and phenolic compounds for basic standardization of Turkish propolis. Applied Biological Chemistry, 64(1). https://doi.org/10.1186/s13765-021-00608-3

Pasupuleti, V. R., Sammugam, L., Ramesh, N., & Gan, S. H. (2017). Honey, Propolis, and Royal Jelly: A Comprehensive Review of Their Biological Actions and Health Benefits. Oxidative Medicine and Cellular Longevity, 2017. https://doi.org/10.1155/2017/1259510

Pobiega, K., Kraśniewska, K., Derewiaka, D., & Gniewosz, M. (2019). Comparison of the antimicrobial activity of propolis extracts obtained by means of various extraction methods. Journal of Food Science and Technology, 56(12), 5386–5395. https://doi.org/10.1007/s13197-019-04009-9

Pujirahayu, N., Bhattacharjya, D. K., Suzuki, T., & Katayama, T. (2019). α-Glucosidase inhibitory activity of cycloartane-type triterpenes isolated from indonesian stingless bee propolis and their structure-activity relationship. Pharmaceuticals, 12(3). https://doi.org/10.3390/ph12030102

Pujirahayu, N., Ritonga, H., & Uslinawaty, Z. (2014). Properties and flavonoids content in propolis of some extraction method of raw propolis. International Journal of Pharmacy and Pharmaceutical Sciences, 6(6), 338–340.

Ririmasse, L., Mokosuli, Y. S., & Manoppo, J. S. (2022). Aktivitas antibakteri ekstrak sarang lebah ( apis dorsata binghami ). 3(1), 40–48.

Rivera-Yañez, N., Rodriguez-Canales, M., Nieto-Yañez, O., Jimenez-Estrada, M., Ibarra-Barajas, M., Canales-Martinez, M. M., & Rodriguez-Monroy, M. A. (2018). Hypoglycaemic and Antioxidant Effects of Propolis of Chihuahua in a Model of Experimental Diabetes. Evidence-Based Complementary and Alternative Medicine, 2018. https://doi.org/10.1155/2018/4360356

Rodiahwati, W., Ariskanopitasari, & Saleh, I. K. (2019). Identification of total bioflavonoid compound of propolis extract from wild honey bee hives apis dorsata in Sumbawa Region, Indonesia. Applied Science and Engineering Progress, 12(1), 37–43. https://doi.org/10.14416/j.ijast.2019.01.007

Rosyidi, D., Eka Radiati, L., Minarti, S., Mustakim, M., Susilo, A., Jaya, F., & Azis, A. (2018). Perbandingan Sifat Antioksidan Propolis pada Dua Jenis Lebah (Apis mellifera dan Trigona sp.) di Mojokerto dan Batu, Jawa Timur, Indonesia. Jurnal Ilmu Dan Teknologi Hasil Ternak, 13(2), 108–117. https://doi.org/10.21776/ub.jitek.2018.013.02.5

Sani, R. N., Nisa, F. C., Andriani, R. D., & Maligan, J. M. (2014). Analisis Rendemen Dan Skrining Fitokimia Ekstrak Etanol Mikroalga Laut Tetraselmis chuii Yield Analysis and Phytochemical Screening Ethanol Extract of Marine Microalgae Tetraselmis chuii. 2(2), 121–126.

Semuel, M. Y., Kaunang, E. S. N., & Manopo, J. S. (2019). The bioactive contents and antioxidant activity of honey bee nest extract of apis dorsata binghami from the North Sulawesi. Molekul, 14(2), 92–102. https://doi.org/10.20884/1.jm.2019.14.2.502

Semuel, M. Y., Mege, R. A., Versya, G., & Rompas, C. (2019). The antihyperlipidemic activity of Apis dorsata Binghami nesting extract in atherogenic diet- induced hyperlipidemic rats. 174–178.

Supeno, B., & Erwan. (2016). Pengenalan Pembelajaran Tentang Lebah Madu ( Honey Bees ).

Šuran, J., Cepanec, I., Mašek, T., & Radi, B. (2021). Propolis Extract and Its Bioactive Compounds — From. Molecules, 26, 1–21. https://doi.org/10.33476/mkp.v11i2.1326

Wa Ode Sri Asnaniar. (2019). DOI: http://dx.doi.org/10.33846/sf10410 Hubungan. Jurnal Penelitian Kesehatan Suara Forikes, 10(4), 295–298.

Yuda, I. P., Ferlianti, R., & Nasrullah, T. (2019). Uji Aktivitas Antidiabetes Infusa Beras Hitam ( Oryza sativa L . indica ) dengan Metode Toleransi Glukosa dan Inhibisi α -Glukosidase Antidiabetic Activity of Black Rice ( Oryza sativa L . indica ) Infusion Using Glucose Tolerance and α -Glucosidase Inhib. 11(2), 123–132.

Yuniarto, A., & Selifiana, N. (2018). Aktivitas Inhibisi Enzim Alfa-glukosidase dari Ekstrak Rimpang Bangle (Zingiber cassumunar Roxb.) secara In vitro. MPI (Media Pharmaceutica Indonesiana), 2(1), 22–25. https://doi.org/10.24123/mpi.v2i1.1299

Wen, C., Zhang, J., Zhang, H., Dzah, C. S., Zandile, M., Duan, Y., ... & Luo, X. (2018). Advances in ultrasound assisted extraction of bioactive compounds from cash crops–A review. Ultrasonics sonochemistry, 48, 538-549. https://doi.org/10.1016/j.ultsonch.2018.07.018




DOI: https://doi.org/10.36987/jpbn.v9i2.4511

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