Bioactive Contents, BSLT Toxicity and antioxidants from Apis dorsata Binghami and Apis mellifera nest extracts

Reinny Silvana Tuegeh, Herry Maurits Sumampouw, Meity Neltje Tanor, Yermia Semuel Mokosuli

Abstract


Beenest is rich in secondary metabolites because honeycombs, among others, are formed from plant resins (propolis). This study aimed to analyze differences in flavonoid content and in vitro antioxidant activity of Apis dorsata Binghami and Apis mellifera nest extracts. The samples used were A. dorsata from North Sulawesi and A. mellifera from South Sulawesi. The honeycomb was extracted using 95% ethanol solvent. Honeycomb extract was analyzed for its flavonoid content by the HPLC method, toxicity was tested by the BSLT method, and in vitro antioxidant activity was tested by the DPPH method. The results of the analysis of flavonoids showed that A. dorsata nest extract produced 21 types of compounds while A. mellifera produced 26 types of compounds. The toxicity test results showed that the A. dorsata nest extract had a better LC50 of 245,691 mg/l than the A. mellifera nest extract with an LC50 of 443,701 mg/l. The in vitro antioxidant test results of A. dorsata nest extract were more robust, namely IC 50 1.161 mg/l, compared to A. mellifera nest extract IC 50 2.404 mg/l. However, both were included in the category of powerful antioxidants. In vitro, anticancer test results on MCF-7 cells, A. mellifera nest extract was active with IC 50 100.02mg/l. Compared to A. dorsata extract, it was active with IC 50 102.217mg/l, but the two were not significantly different. Based on the analysis of flavonoid content, toxicity test, and antioxidant test, A.dorsata and A.mellifera honeycomb extracts have potential as in vitro antioxidants

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Abdullah, N. A., Zullkiflee, N., Zaini, S. N. Z., Taha, H., Hashim, F., & Usman, A. (2020). Phytochemicals, mineral contents, antioxidants, and antimicrobial activities of propolis produced by Brunei stingless bees Geniotrigona thoracica, Heterotrigona itama, and Tetrigona binghami. Saudi Journal of Biological Sciences, 27(11), 2902-2911.

Alvear, M., Santos, E., Cabezas, F., Pérez-SanMartín, A., Lespinasse, M., & Veloz, J. (2021). Geographic Area of Collection Determines the Chemical Composition and Antimicrobial Potential of Three Extracts of Chilean Propolis. Plants, 10(8), 1543.

Amalia, E., Diantini, A., & Subarnas, A. (2020). Water soluble propolis and bee pollen of Trigona spp. from South Sulawesi Indonesia induce apoptosis in the human breast cancer MCF 7 cell line. Oncology Letters, 20(5), 1-1.

Asem, N., Abdul Gapar, N. A., Abd Hapit, N. H., & Omar, E. A. (2020). Correlation between total phenolic and flavonoid contents with antioxidant activity of Malaysian stingless bee propolis extract. Journal of Apicultural Research, 59(4), 437-442.

Bankova VS, de Castrob SL, & Marcuccic MC. 2000. Propolis: recent advances in chemistry and plant origin. Apidologie, 31, 3-15.

Béji-Srairi, R., Younes, I., Snoussi, M., Yahyaoui, K., Borchard, G., Ksouri, R., ... & Wided, M. K. (2020). Ethanolic extract of Tunisian propolis: chemical composition, antioxidant, antimicrobial and antiproliferative properties. Journal of Apicultural Research, 1-11.

Berretta, A. A., Silveira, M. A. D., Capcha, J. M. C., & De Jong, D. (2020). Propolis and its potential against SARS-CoV-2 infection mechanisms and COVID-19 disease. Biomedicine & Pharmacotherapy, 110622.

Bouchelaghem, S., Das, S., Naorem, R. S., Czuni, L., Papp, G., & Kocsis, M. (2022). Evaluation of total phenolic and flavonoid contents, antibacterial and antibiofilm activities of Hungarian Propolis ethanolic extract against Staphylococcus aureus. Molecules, 27(2), 574.

Cahyaningsih, E., Yuda, P. E. S. K., & Santoso, P. (2019). Skrining fitokimia dan uji aktivitas antioksidan ekstrak etanol bunga telang (Clitoria ternatea L.) dengan metode spektrofotometri uv-Vis. Jurnal Ilmiah Medicamento, 5(1).

Cornara, L., Biagi, M., Xiao, J., Burlando, B. (2017) Therapeutic Properties of Boactive Compounds from different Honeybee Product. Frontiers in Pharmacology, 8 (JUN), 1-20.

Diva, A. N., Pratami, D. K., Wijanarko, A., Hermansyah, H., & Sahlan, M. (2019, April). Effect of ethanolic propolis extract from Tetragonula biroi bees on the growth of human cancer cell lines HeLa and MCF-7. In AIP Conference Proceedings (Vol. 2092, No. 1, p. 030002). AIP Publishing LLC.

Dos Santos, D. A., Munari, F. M., da Silva Frozza, C. O., Moura, S., Barcellos, T., Henriques, J. A. P., & Roesch-Ely, M. (2019). Brazilian red propolis extracts: study of chemical composition by ESI-MS/MS (ESI+) and cytotoxic profiles against colon cancer cell lines. Biotechnology Research and Innovation, 3(1), 120-130.

Forma, E., & BryÅ›, M. (2021). Anticancer activity of propolis and its compounds. Nutrients, 13(8), 2594.

Gajek, G.; Marciniak, B.; Lewkowski, J.; Kontek, R. Antagonistic effects of CAPE (a Component of Propolis) on the cytotoxicity and genotoxicity of irinotecan and SN38 in Human gastrointestinal cancer cells in vitro. Molecules 2020, 25, 658

Galeotti F, Maccari F, Fachini A and Volpi N. Chemical Composition and Antioxidant Activity of Propolis Prepared in Different Forms and in Different Solvents Useful for Finished Products. Foods 2018, 7, 41; doi:10.3390/foods7030041

Kocot, J., Kielczykowska, M., Luchowska-Kocot, D., Kurzepa, J., Musik I. (2018). Antioksidant Potential of Propolis, Bee Pollen, and Royal Jelly: Possible Medical Aplication. Oxidative Medicine and Cellular Longevity

Kurek-Górecka, A., Keskin, Ş., Bobis, O., Felitti, R., Górecki, M., Otręba, M., ... & Rzepecka-Stojko, A. (2022). Comparison of the antioxidant activity of propolis samples from different geographical regions. Plants, 11(9), 1203.

Mangiring, G. A., Pratami, D. K., Hermansyah, H., Wijanarko, A., Rohmatin, E., & Sahlan, M. (2018, February). Microencapsulation of ethanol extract propolis by maltodextrin and freeze-dried preparation. In AIP Conference Proceedings (Vol. 1933, No. 1, p. 020012). AIP Publishing LLC.

Misir, S., Aliyazicioglu, Y., Demir, S., Turan, I., & Hepokur, C. (2020). Effect of Turkish propolis on miRNA expression, cell cycle, and apoptosis in human breast cancer (MCF-7) cells. Nutrition and cancer, 72(1), 133-145.

Mokosuli YS, Pelealu J, Tulung M dan Mandey LC. 2013. Mokosuli YS and Repi RA. 2015. The Characteristics of Bioactive peptides and antibacterial activity of honey bee Apis nigrocincta Smith Venom Endemic To Sulawesi. Molekul, Vol. 10. No2. : 135-144

Mokosuli YS, Pelealu J, Tulung M dan Mandey LC. 2013a. Karakter Morfologi, Sumber Pakan, dan Bioaktiviotas Farmakologis Racun Lebah Madu Endemik Sulawesi Apis dorsata Binghami dan Apis nigrocincta Smith [HYMENOPTERA : Apidae]. Disertasi Program Pascasarjana Universitas Sam Ratulangi, Manado.

Mokosuli YS, Pelealu J, Tulung M dan Mandey LC. 2013b. Pharmacological Bioactivity Honey Bee Venom Apis nigrocincta Smith and Apis dorsata Binghami Endemic To Sulawesi. Int. J. Science and Engginering Inv. Vol. 2. Issue 18: 25-33.

Mokosuli, Y. S. (2021). Brine Shrimp Lethality Test (BSLT) Ekstrak Sarang Lebah Madu (Apis dorsata Binghami. Jurnal Pendidikan Biologi undiksha, 8(3), 138-144.

Najafpour Darzi, G., Heidari, G., Mohammadi, M., & Moghadamnia, A. A. (2019). Microwave ultrasound assisted extraction: determination of quercetin for antibacterial and antioxidant activities of Iranian propolis. International Journal of Engineering, 32(8), 1057-1064.

Okińczyc, P., Widelski, J., Szperlik, J., Żuk, M., Mroczek, T., Skalicka-Woźniak, K., ... & Kuś, P. M. (2021). Impact of plant origin on eurasian propolis on phenolic profile and classical antioxidant activity. Biomolecules, 11(1), 68.

Pasupuleti, V. R., Sammugam, L., Ramesh, N., Gan, S.H. (2017). Honey, Propolis, and Royal Jelly: A Comprehensive Review of Their Biological Actions and Health Benefits

Przybyłek I and Karpinski TM. 2019. Antibacterial Properties of Propolis. Molecules 2019, 24, 2047; doi:10.3390/molecules24112047

Pujirahayu, N., Ritonga, H. & Uslinawaty, Z. (2014). Properties and Flavonoids Content In Propolis of Some Extraction Methode of Raw Propolis. International Journal of Pharmacy and Pharmaceutical Sciencess 6 ISSN.0975- 1491

Rivera-Yanez, N., Rivera-Yanez, C. R., Pozo Molina G. Mendez-Catala, Mendez CruzA.R. (2021) Biomedical Properties of Propolis on Diverse Chronic Disease and its Potential Applications and Health Benefits. Nutrient, 13(1),1-31

Safitri, E., Purnobasuki, H., Purnama, M.T.E., Chhetri, S., (2022) Effectiveness of Forest Honey (Apis dorsata) as Therapy for ovarian Failure Causing Malnutrition. F1000Research, 11, 512.

Sarıkahya, N. B., Gören, A. C., Okkalı, G. S., Çöven, F. O., Orman, B., Kırcı, D., ... & Nalbantsoy, A. (2021). Chemical composition and biological activities of propolis samples from different geographical regions of Turkey. Phytochemistry Letters, 44, 129-136.

Semuel, M. Y., & Rombot, D. O. (2023). Bioactivity of Apis dorsata Nest Extract from a Different Geographical Location in Minahasa, North Sulawesi, Indonesia. International Journal of Agriculture and Biology, 29(1), 1-8.

Sforcin, J M; Bankova, V (2011) Propolis: Is there a potential for the development of new drugs? J. Ethnopharmacol 133: 253-260.

Sitepu, K., Arianto, A., Ginting, L. R. B., & Damanik, H. D. (2021). Tindakan Kemoterapi Dengan Tingkat Kecemasan Pada Klien Kanker Di Rumah Sakit Grandmed Lubuk Pakam Tahun 2021. Jurnal Pengmas Kestra (Jpk), 1(1), 180-185.

Sobral, F., Calhelha, R. C., Barros, L., Dueñas, M., Tomás, A., Santos-Buelga, C.& Ferreira, I. C. (2017). Flavonoid composition and antitumor activity of bee bread collected in northeast Portugal. Molecules, 22(2), 248.

Syed Salleh, S. N. A., Mohd Hanapiah, N. A., Ahmad, H., Wan Johari, W. L., Osman, N. H., & Mamat, M. R. (2021). Determination of total phenolics, flavonoids, and antioxidant activity and GC-MS analysis of Malaysian stingless bee propolis water extracts. Scientifica, 2021.

Veloz JJ, Saavedra N, Alvear M, Zambrano T, Barrientos L and Salazar LA. 2016. Polyphenol-Rich Extract from Propolis Reduces the Expression and Activity of Streptococcus mutans Glucosyltransferases at Subinhibitory Concentrations. BioMed Research International, Volume 2016, Article ID 4302706, 7 pages, http://dx.doi.org/10.1155/2016/4302706

Wang, X., Sankarapandian, K., Cheng, Y., Woo, S. O., Kwon, H. W., Perumalsamy, H., & Ahn, Y. J. (2016). Relationship between total phenolic contents and biological properties of propolis from 20 different regions in South Korea. BMC complementary and alternative medicine, 16, 1-12.

Wen, C., Zhang, J., Zhang, H., Dzah, C. S., Zandile, M., Duan, Y., ... & Luo, X. (2018). Advances in ultrasound assisted extraction of bioactive compounds from cash crops–A review. Ultrasonics sonochemistry, 48, 538-549.

Zabaiou, N.; Fouache, A.; Trousson, A.; Buñay-Noboa, J.; Marceau, G.; Sapin, V.; Zellagui, A.; Baron, S.; Lahouel, M.; Lobaccaro, J.M.A. Ethanolic extract of Algerian propolis decreases androgen receptor transcriptional activity in cultured LNCaP cells. J. Steroid Biochem. Mol. Biol. 2019, 189, 108–115




DOI: https://doi.org/10.36987/jpbn.v9i2.4514

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