Potential And Mechanism Antagonistic of Trichoderma sp. Againts Colletotrichum gloeosporioides and C. aotearoa on Avocado Plant (Persea americana Mill)

Luluk Rofiatul Mafluhah, Utami Sri Hastuti, Sitoresmi Prabaningtyas

Abstract


The objective of this study is to ascertain the pathogenic and antagonistic fungi that induce disease in avocado plants (Persea Americana Mill), identify the antagonistic fungi with the greatest antagonism potential among a variety of antagonistic fungi, and elucidate the mechanism by which antagonistic fungi inhibit the growth of pathogenic fungi. From January to May 2023, this study was conducted in vitro at the Microbiology Laboratory, State University of Malang, employing a completely randomized design (CRD) comprising six replications and four interventions. The identification of mold was accomplished through macroscopically and microscopically descriptive analysis, followed by a comparison with the mold identification key book. The antagonism process was executed utilizing the dual culture method on PDA medium, and the electron microscope (SEM) was employed to witness the antagonism mechanism. The research findings revealed that T. harzianum spp. and T. viride were identified as antagonist fungi, whereas C. aotearoa and C. gloeospoiroides were identified as pathogenic fungi. The antagonistic fungus T. viride exhibited the greatest degree of antagonism, impeding the growth of C. gloeospoioroides by 77.8%. Observed antagonism occurs when the hyphae of the antagonistic mold ensnare, entangle, and pierce those of the pathogenic mold, causing harm to the region surrounding the pathogenic mold's hyphae and subsequently impeding its growth

Keywords


Antagonism power, Antagonism mechanism, Trichoderma sp., C. gloeospoiroides, C. aotearoa

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References


Dharmaputra, O. S., Gunawan, A. W., Wulandari, R., & Basuki, T. (1999). Cendawan Kontaminan Dominan pada Bedengan Jamur Merang dan Interaksinya dengan Jamur Merang Secara in vitro Dominant Fungi Contaminating the Beds ofRice Straw Mushroom and Their Interaction with Straw Mushroom in vitro. Jurnal Mikrobiotogi Indonesia. Februari, 4(1), 14–18.

Dreher, M. L., & Davenport, A. J. (2013). Hass Avocado Composition and Potential Health Effects. Critical Reviews in Food Science and Nutrition, 53(7), 738–750. https://doi.org/10.1080/10408398.2011.556759

Es-Soufi, R., Tahiri, H., Azaroual, L., Oualkadi, A. El, Martin, P., Badoc, A., Lamarti, A., Es-Soufi, R., Tahiri, H., Azaroual, L., Oualkadi, A. El, Martin, P., Badoc, A., & Lamarti, A. (2020). Biocontrol Potential of Bacillus amyloliquefaciens Bc2 and Trichoderma harzianum TR against Strawberry Anthracnose under Laboratory and Field Conditions. Agricultural Sciences, 11(3), 260–277. https://doi.org/10.4236/AS.2020.113017

Hastuti, U. S., Aisaroh, S., & Najib, A. (2008). Daya Antagonisme Trichoderma spp. Terhadap Beberapa Spesies Kapang Patogen dari Rhizosfer Tanah Pertanian Kedelai. Seminar Nasional X Pendidikan Biologi FKIP UNS, 2000, 1–5. https://media.neliti.com/media/publications/174674-ID-daya-antagonisme-trichoderma-spp-terhada.pdf

Hastuti, U. S. (2017) Antagonisme antara Kapang Antagonis dan Kapang Patogen. Malang. UMM Press

Khare, E., Kumar, S., & Kim, K. (2018). Role of peptaibols and lytic enzymes of Trichoderma cerinum Gur1 in biocontrol of Fusraium oxysporum and chickpea wilt. Environmental Sustainability, 1(1), 39–47. https://doi.org/10.1007/s42398-018-0001-7

Kimaru, S. K., Monda, E., Cheruiyot, R. C., Mbaka, J., & Alakonya, A. (2018). Morphological and Molecular Identification of the Causal Agent of Anthracnose Disease of Avocado in Kenya. International Journal of Microbiology, 2018. https://doi.org/10.1155/2018/4568520

Kumar, K., Amaresan, N., Bhagat, S., Madhuri, K., & Srivastava, R. C. (2012). Isolation and Characterization of Trichoderma spp. for Antagonistic Activity Against Root Rot and Foliar Pathogens. Indian Journal of Microbiology, 52(2), 137–144. https://doi.org/10.1007/s12088-011-0205-3

Lestari, R., Agus Sukamto, L., Aprilianti, P., Wahyuni, S., & Putri, W. U. (2016). Selection of avocado plants based on fruit characters, fat content, and continual harvest along the year in West Java-Indonesia. International Journal on Advanced Science, Engineering and Information Technology, 6(1), 77–83. https://doi.org/10.18517/ijaseit.6.1.621

María Teresa, R. C., Rosaura, V. G., Elda, C. M., & Ernesto, G. P. (2014). The avocado defense compound phenol-2,4-bis (1,1-dimethylethyl) is induced by arachidonic acid and acts via the inhibition of hydrogen peroxide production by pathogens. Physiological and Molecular Plant Pathology, 87, 32–41. https://doi.org/10.1016/J.PMPP.2014.05.003

Muhibbudin, A., Salsabila, S., Sektiono, A. W., Pertanian, F., Brawijaya, U., & Timur, J. (2021). Kemampuan Antagonis Tricoderma harzianum Terhadap Beberapa Jamur Patogen Penyakit Tanaman. Agrosaintifika: Ilmu-Ilmu Pertanian, 4(1), 225–233.

Muhibuddin, A., Setiyowati, E. M., & Sektiono, A. W. (2022). Mechanism Antagonism Of Trichoderma viride Against Several Types Of Pathogens And Production Of Secondary Metabolites. Agrosaintifika, 4(1), 243–253. https://doi.org/10.32764/agrosaintifika.v4i1.2375

Ningsih, H., Hastuti, U. S., & Listyorini, D. (2016). Kajian Antagonis Trichoderma Spp . terhadap Fusarium Solani Penyebab Penyakit Layu Pada Daun Cabai Rawit (Capsicum frutescens) Secara in Vitro. Proceeding Biology Education Conference, 13(1), 814–817.

Purwantisari, S., & Evendi, A. (2015). The Potential Test of Fungal Antagonist Trichoderma viride to inhibit the Growth of Pathogenic Fungi Fusarium moniliforme and Alternaria solani In-Vitro. Jurnal Sains Dan Matematika, 23(3), 73–77.

Schubert, M., Fink, S., & Schwarze, F. W. M. R. (2008). In vitro screening of an antagonistic trichoderma strain against wood decay fungi. Arboricultural Journal, 31(4), 227–248. https://doi.org/10.1080/03071375.2008.9747541

Sood, M., Kapoor, D., Kumar, V., Sheteiwy, M. S., Ramakrishnan, M., Landi, M., Araniti, F., & Sharma, A. (2020). Trichoderma: The “Secrets†of a Multitalented Biocontrol Agent. Plants (Basel, Switzerland), 9(6), 1–25. https://doi.org/10.3390/PLANTS9060762

Swain, H., Adak, T., Mukherjee, A. K., Mukherjee, P. K., Bhattacharyya, P., Behera, S., Bagchi, T. B., Patro, R., Shasmita, Khandual, A., Bag, M. K., Dangar, T. K., Lenka, S., & Jena, M. (2018). Novel Trichoderma strains isolated from tree barks as potential biocontrol agents and biofertilizers for direct seeded rice. Microbiological Research, 214, 83–90. https://doi.org/10.1016/J.MICRES.2018.05.015

Tanama, A., Hastuti, U. S., & Gofur, A. (2020). Pengembangan Handout Mikrobiologi Berdasarkan Hasil Penelitian Antagonisme Kapang Antagonisme Kapang Antagonis Terhadap Kapang Patogen pada Tanaman Buah Naga (Hylocereus sp). Bioscientist: Jurnal Ilmiah Biologi, 8(2), 236–243.

Triasih, U., Abadi, A. L., & Muhibbudin, A. (2022). Uji Beberapa Jamur Antagonis Terhadap Colletotrichum gloeosporiodes Penyebab Penyakit Busuk Buah Apel Manalagi ( Malus sylvestris ) Secara In Vitro. Transformasi Pertanian Digital Dalam Mendukung Ketahanan Pangan Dan Masa Depan Yang Berkelanjutan, 389–397. https://doi.org/10.25047/agropross.2022.309

Triasih, U., Wuryantini, S., & Agustina, D. (2022). Karakterisasi Cendawan Rizosfer Kebun Jeruk Organik dan Potensinya dalam Menghambat Pertumbuhan Botryodiplodia theobromae dan Colletotrichum gloeosporioides Characterization of Soil Rhizospheric Fungi on Citrus Plantation and Their Potential to Inhibiting. Jurnal Fitopatologi Indonesia, 18(September), 205–212. https://doi.org/10.14692/jfi.18.5.

Valle-Aguirre, G., Velázquez-del Valle, M. G., Corona-Rangel, M. L., Amora-Lazcano, E., & Hernández-Lauzardo, A. N. (2016). First aeromycological study in an avocado agroecosystem in Mexico. Aerobiologia, 32(4), 657–667. https://doi.org/10.1007/s10453-016-9439-1

Živković, S., Stojanović, S., Ivanović, Ž., Gavrilović, V., Popović, T., & Balaž, J. (2010). Serbian Source Colletotrichum acutatum Colletotrichum gloeosporioides. Archives of Biological Sciences, 62(3), 611–623. https://doi.org/10.2298/ABS1003611Z




DOI: https://doi.org/10.36987/jpbn.v9i3.4689

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