Optimation of Green Synthesis Biopolymer Cellulose Using Acetobacter xylinum From Whey as Media of Bacteria

Suharni Sinaga, Junius Gian Ginting, Helen Anjelina Simanjuntak, Dame Meldaria sipahutar, Defacto Firmawai Zega, Yanti Yanti, Sahat Sinaga, Lydia Barus, Hermawan Purba

Abstract


Utilizing waste into a more valuable commodity was one of the objectives of that research. Waste which initially became an environmental pollutant factor could be processed into more useful products. Tofu liquid waste was referred to whey was a by-product of tofu production. Whey was waste that still contains organic materials such as protein, fat, carbohydrates and minerals. That whey was used as a growth medium for Acetobacter xylinum bacteria and produces cellulose biopolymer as a result of carbohydrate fermentation. The process of optimizing carbohydrate fermentation by Acetobacter xylinum bacteria was carried out by varying the ratio of the amount of whey to coconut water, the acidity level of the media, and the fermentation time. The research results showed that the biosynthesis of cellulose biopolymer was obtained from a ratio of whey concentration to coconut water of 1:1, variations in acidity levels showed that the optimum fermentation conditions occurred at pH 4, and the optimal amount of cellulose biopolymer was obtained on day 21, namely with the highest percent yield value. The resulting biocellulose was characterized using FTIR, XRD, and SEM. The characterization results show that biocellulose is well formed with a fiber surface shape and particle size in the range of 50-200 nm

Keywords


Acetobacter xynlinum, Biopolymer Cellulose, Green Synthesis, Optimation

Full Text:

PDF

References


Chua, J. Y., & Liu, S. Q. (2019). Soy whey: More than just wastewater from tofu and soy protein isolate industry. Trends in Food Science and Technology, 91(June), 24–32. https://doi.org/10.1016/j.tifs.2019.06.016

Costa, A. F. S., Almeida, F. C. G., Vinhas, G. M., & Sarubbo, L. A. (2017). Production of bacterial cellulose by Gluconacetobacter hansenii using corn steep liquor as nutrient sources. Frontiers in Microbiology, 8(OCT), 1–12. https://doi.org/10.3389/fmicb.2017.02027

Dincă, V., Mocanu, A., Isopencu, G., Busuioc, C., Brajnicov, S., Vlad, A., Icriverzi, M., Roseanu, A., Dinescu, M., Stroescu, M., Stoica-Guzun, A., & Suchea, M. (2020). Biocompatible pure ZnO nanoparticles-3D bacterial cellulose biointerfaces with antibacterial properties. Arabian Journal of Chemistry, 13(1), 3521–3533. https://doi.org/10.1016/j.arabjc.2018.12.003

Esa, F., Tasirin, S. M., & Rahman, N. A. (2014). Overview of Bacterial Cellulose Production and Application. Agriculture and Agricultural Science Procedia, 2, 113–119. https://doi.org/10.1016/j.aaspro.2014.11.017

Fransiska, H. purba. (2021). Glukosa; Aspek analisis dan Kesehatan Pada Ibu dan Anak.

Hasan, A. M., Uno, W. D., Simanjuntak, H. A., Purba, H., Pasaribu, C., Ginting, G., Situmorang, T. S., Sfarmasi, S., Tinggi, S., Kesehatan, I., & Senior, S. (2023). Potensi Buah Andaliman ( Zanthoxylum acanthopodium DC .) sebagai Antibakteri Terhadap Salmonella typhi Potential of Andaliman Fruit ( Zanthoxylum acanthopodium DC .) as Antibacterial Against Salmonella typhi. 4(2), 86–93. https://doi.org/10.34007/jonas.v4i2.404

Hassan, E., Hassan, M., Abou-zeid, R., Berglund, L., & Oksman, K. (2017). Use of bacterial cellulose and crosslinked cellulose nanofibers membranes for removal of oil from oil-in-water emulsions. Polymers, 9(9). https://doi.org/10.3390/polym9090388

Irham, W. H., Tamrin, Marpaung, L., & Marpongahtun. (2020). Characterization of bacterial cellulose from coconut water supplemented curcuma longa linn and ziziphus mauritiana extract. AIP Conference Proceedings, 2267(September 2020). https://doi.org/10.1063/5.0023953

Lu, H., & Jiang, X. (2014). Structure and properties of bacterial cellulose produced using a trickling bed reactor. Applied Biochemistry and Biotechnology, 172(8), 3844–3861. https://doi.org/10.1007/s12010-014-0795-4

Marpongahtun, Gea, S., Muis, Y., Andriayani, Novita, T., & Piliang, A. F. (2018). Synthesis of Carbon Nanodots from Cellulose Nanocrystals Oil Palm Empty Fruit by Pyrolysis Method. Journal of Physics: Conference Series, 1120(1), 6–11. https://doi.org/10.1088/1742-6596/1120/1/012071

Nurhasmawaty, P. (2020). Pengolahan Limbah Cair Industri Tahu dengan Proses Biofilter Aerobik. Jurnal Teknik Kimia, 1–82.

Pagoray, H., Sulistyawati, S., & Fitriyani, F. (2021). Limbah Cair Industri Tahu dan Dampaknya Terhadap Kualitas Air dan Biota Perairan. Jurnal Pertanian Terpadu, 9(1), 53–65. https://doi.org/10.36084/jpt..v9i1.312

Pradana, T. D., Suharno, S., & Apriansyah, A. (2018). Pengolahan Limbah Cair Tahu Untuk Menurunkan Kadar TSS Dan BOD. Jurnal Vokasi Kesehatan, 4(2), 56. https://doi.org/10.30602/jvk.v4i2.9

Purba, H., Marpongahtun, Andriayani, Septevani, A. A., & Sitepu, E. (2023). Charaterization of Nanocellulose from Liquid Wasted of Tofu by Acetobacter xylinum. AIP Conference Proceedings, 2626(August). https://doi.org/10.1063/5.0136106

Purba, H., Simanjuntak, H. A., & Situmorang, R. (2020). Phytochemical screening of bunga rosella (Hibiscus sabdariffa L) and antimicrobial activity test. Jurnal Pendidikan Kimia, 12(2), 70–78. https://doi.org/10.24114/jpkim.v12i2.19398

Purba, H., Suratman, A., Sugiharto, E., & Sciences, N. (2018). Validation of Spectrophotometric Method for Analysis of Anionic Surfactant Dodecyl Benzene Sulphonate ( DBS ) in Catfish ( Clarias batrachus ) Using Malachite Green. 5(2), 483–487.

Purba hermawan, sinagar bonar, Tinggi, S., Kesehatan, I., Medan, S., Purba, H., Sinaga, B., & Ginting, J. G. (2023). Validasi Metode Analisa Kadar Fenolik Ekstrak Etanol Jahe Merah (Zingiber officinale var rubrum rhizoma) dengan Spektroscopy UV-Vis. 6, 2023.

Wang, J., Tavakoli, J., & Tang, Y. (2019a). Bacterial cellulose production, properties and applications with different culture methods – A review. Carbohydrate Polymers, 219(February), 63–76. https://doi.org/10.1016/j.carbpol.2019.05.008




DOI: https://doi.org/10.36987/jpbn.v9i3.5067

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Hermawan Purba

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Jurnal Pembelajaran dan Biologi Nukleus (JPBN) by LPPM Universitas Labuhanbatu is under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY - SA 4.0). Official contact: Rivo +6281362238917