Effectiveness of Bottle Size Variation on the Growth of Dendrobium Striaenopsis Orchid Plantlets
Abstract
The production of Dendrobium striaenopsis orchid cultivation in Indonesia has many obstacles including limited seed provision, low seed quality and cultivation techniques that have not been done well, while orchid enthusiasts always increase every year. Propagation of orchid plants by conventional means requires a long time to obtain a large number of plant saplings, therefore alternative cultivation using tissue culture techniques is needed. In vitro tissue culture techniques are widely used as a fast and efficient way to produce D. striaenopsis orchid plants, but not infrequently there are factors that affect the production of orchids, one of which is bottle size. The purpose of this study is to determine the effect of the effectiveness of bottle size variations on the growth of D. striaenopsis plantlets after comparing the difference in bottle volume and knowing the optimum size of bottles used for D. striaenopsis plantlet growth. The study was conducted from May to August 2023 in the Laboratory CV. Candi Orchid, Semarang City. The method used was the observation and experimental method after which it was continued with the One Way Anova test and further test T. In this study using Vacin and Went medium with a size of 60 ml by providing treatment variations in bottle sizes of 250 ml, 500 ml and 750 ml with 4 repeats. The result of this study is that a 750 ml bottle used in the tissue culture technique of D. striaenopsis Orchid is effective in use even though the growth of plantlets does not occur a significant difference, this is due to environmental factors, namely light intensity and duration of irradiation
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Ambarwati, I. D., Alfian, F. N., & Dewanti, P. (2021). Respon Anggrek Dendrobium sp., Oncidium sp., dan Phalaenopsis sp. Terhadap Pemberian Empat Jenis Nutrisi Organik yang Berbeda pada Tahap Regenerasi Planlet. Agrikultura, 32(1). https://doi.org/10.24198/agrikultura.v32i1.32366
Apriliyania, R., & Wahidah, B. F. (2021). Perbanyakan anggrek Dendrobium sp. secara in vitro: Faktor-faktor keberhasilannya. https://doi.org/10.24252/filogeni.v1i1.21192
Ayuningtyas, U., Budiman, & Azmi, T. K. K. (2020). Pengaruh Pupuk Daun Terhadap Pertumbuhan Bibit Anggrek Dendrobium Dian Agrihorti Pada Tahap Aklimatisasi. Jurnal Pertanian Presisi (Journal of Precision Agriculture), 4(2). https://doi.org/10.35760/jpp.2020.v4i2.2888
Buruh, P. M., Melalui, M., Budidaya, I., Dendrobium, A., Dengan, S., Jenis, B., Tanam, M., Pupuk, D. A., Mikoriza, H., Kabupaten Banyumas,
Herliana D, O., Harjoso, T., Rokhminarsi, E., Agroekologi, L., Agroteknologi, J., Pertanian, F., Soedirman, J., Agronomi, L., & Hortikultura, D. (2019). Pemberdayaan Mantan Buruh Migran Melalui Introduksi Budidaya Anggrek Dendrobium sp. Dengan Berbagai Jenis Media Tanam dan Aplikasi Pupuk HAayati Mikoriza di Kabupaten Banyumas. Panrita Abdi - Jurnal Pengabdian Pada Masyarakat, 3(1).
“Fandani, H. S., Mallomasang, S. N., & Korja, I. N. (2018). Keanekaragaman Jenis Anggrek pada beberapa Penangkaran di Desa Ampera dan Desa Karunia Kecamatan Palolo Kabupaten Sigi. Jurnal Warta Rimba, 6(9).
Hartati, S., Yunus, A., Cahyono, O., & Setyawan, B. A. (2019). Penerapan Teknik Pemupukan pada Aklimatisasi Anggrek Hasil Persilangan Vanda di Kecamatan Matesih Kabupaten Karanganyar. PRIMA: Journal of Community Empowering and Services, 3(2). https://doi.org/10.20961/prima.v3i2.37905
Herliana, O., Rokhminarsi, E., Mardini, S., & Jannah, M. (2018). Pengaruh jenis media tanam dan aplikasi pupuk hayati mikoriza terhadap pertumbuhan, pembungaan dan infeksi mikoriza pada tanaman anggrek Dendrobium sp. Kultivasi, 17(1). https://doi.org/10.24198/kultivasi.v17i1.15774
Irsyadi, M. B. (2021). Factors That Effect of the Optimal Plantlet Growth from Tissue Culture on the Acclimatization Stage. Proc. Internat. Conf. Sci. Engin, 4(February).
Karimah, N., Kusmiyati, F., & Anwar, S. (2022). Pengaruh Penggunaan Sukrosa dan IBA Terhadap Induksi Akar Eksplan Tunas Anggrek (Dendrobium sp.) Secara in Vitro. AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 5(1). https://doi.org/10.33096/agrotek.v5i1.157
Kartiman, R., Sukma, D., Aisyah, S. I., & Purwito, A. (2018). Multiplikasi in Vitro Anggrek Hitam (Coelogyne pandurata Lindl.) Pada Perlakuan Kombinasi NAA dan BAP. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 5(1). https://doi.org/10.29122/jbbi.v5i1.2908
Latifah, R., Suhermiatin, T., & Ermawati, N. (2017). Optimasi Pertumbuhan Plantlet Cattleya Melalui” Kombinasi Kekuatan Media Murashige-Skoog dan Bahan Organik. Agriprima : Journal of Applied Agricultural Sciences, 1(1). https://doi.org/10.25047/agriprima.v1i1.20
Maitra, S. (2020). Growing of Dendrobium Orchids in Greenhouse. In Protected Cultivation and Smart Agriculture. https://doi.org/10.30954/ndp-pcsa.2020.30
Nurana, A. R., Wijana, G., & Dwiyani, R. (2017). Pengaruh 2-iP dan NAA terhadap Pertumbuhan Plantlet Anggrek Dendrobium Hibrida pada Tahap Subkultur. Agrotrop, 7(2).
Rosanti, D., & Widianjaya, R. R. (2018). Morfologi Orchidaceae di Kebun Raya Liwa Kabupaten Lampung Barat Provinsi Lampung. Sainmatika: Jurnal Ilmiah Matematika Dan Ilmu Pengetahuan Alam, 15(2). https://doi.org/10.31851/sainmatika.v15i2.2371
Saepudin, A., Yulianto, Y., & Aeni, R. N. (2020). Pertumbuhan Eksplan In Vitro Anggrek Hibrida Dendrobium Pada Beberapa Media Dasar dan Konsentrasi Air Kelapa. Media Pertanian, 5(2). https://doi.org/10.37058/mp.v5i2.2451
Shidiqy, H. A., Wahidah, B. F., & Hayati, N. (2019). Karakterisasi Morfologi Anggrek (Orchidaceae) di Hutan Kecamatan Ngaliyan Semarang. Al-Hayat: Journal of Biology and Applied Biology, 1(2). https://doi.org/10.21580/ah.v1i2.3761
Takatsuka, H., Higaki, T., & Umeda, M. (2018). Actin reorganization triggers rapid cell elongation in roots. Plant Physiology, 178(3). https://doi.org/10.1104/pp.18.00557
Tuhuteru, S., Hehanussa, M. L., & Raharjo, S. H. T. (2018). Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa. Agrologia, 1(1). https://doi.org/10.30598/a.v1i1.293
Yuniardi, F. (2020). Aplikasi Dimmer Switch pada Rak Kultur Sebagai Pengatur Kebutuhan Intesitas Cahaya Optimum Bagi TanamanIn Vitro. Indonesian Journal of Laboratory, 1(4). https://doi.org/10.22146/ijl.v1i4.52991
DOI: https://doi.org/10.36987/jpbn.v10i1.5325
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