Browning Prevention Method in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in Vitro Culture

Devi Sonti Sibarani, Ratih Restiani, Aniek Prasetyaningsih

Abstract


Browning of explants is a common problem in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in vitro culture, resulting in low explant in vitro regeneration of Kepel. Using nodal explant, the effect of explant immersion in ascorbic acid, the addition of ascorbic acid to the media, and dark and light incubation conditions were investigated. The browning prevention method was selected based on the delayed browning appearance time, the lowest broning intensity, and the highest percentage of callus. This present study used a completely randomized design with treatment variations: immersion explant in ascorbic acid 100 mg/L, addition of ascorbic acid 100 and 200 mg/L to the media, addition of ascorbic acid 100 and 200 mg/L and activated charcoal 1 g/L to the media, and incubation in dark and light conditions for 28 days. The results showed that the combination of the addition of ascorbic acid 200 mg/L to MS media and incubation in dark conditions were effective browning prevention methods in inhibiting browning appearance time (5 DAP), significantly reducing browning intensity (0.3), and increasing callus growth (100%) of kepel node explants during 28 days of culture. The results of this study are useful in establishing protocols for in vitro culture of Kepel plants, especially at the initiation stage, and can be expected to support the successful conservation of Kepel plants through in vitro propagation.

 

Keywords: Ascorbic acid, Activated Charcoal, Browning, In Vitro Culture, Stelechocarpus burahol


Keywords


Ascorbic acid, Activated Charcoal, Browning, In Vitro Culture, Stelechocarpus burahol

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References


Abdalla, N., El-Ramady, H., Seliem, M. K., El-Mahrouk, M. E., Taha, N., Bayoumi, Y., Shalaby, T. A., & Dobránszki, J. (2022). An Academic and Technical Overview on Plant Micropropagation Challenges. Horticulturae, 8(8). https://doi.org/10.3390/horticulturae8080677

Admojo, L., & Indrianto, A. (2016). Pencegahan Browning Fase Inisasi Kalus Pada Kultur Midrib Daun Klon Karet (Hevea brasiliensis Muell Arg) Pb 330. Jurnal Penelitian Karet, 3, 25–34. https://doi.org/10.22302/ppk.jpk.v34i1.220

Amente, G., & Chimdessa, E. (2021). Control of browning in plant tissue culture: A review. Journal of Scientific Agriculture, 5, 67–71. https://doi.org/10.25081/jsa.2021.v5.7266

Angio, M. H., & Firdiana, E. R. (2021). Kepel (Stelechocarpus burahol (Blume) Hook & Thompson), buah langka khas keraton Yogyakrta: sebuah koleksi kebun raya Purwodadi. Warta Kebun Raya, 19(2), 7–13.

Cai, X., Wei, H., Liu, C., Ren, X., Thi, L. T., & Jeong, B. R. (2020). Synergistic effect of NaCl pretreatment and PVP on browning suppression and Callus induction from petal explants of Paeonia lactiflora pall. ‘Festival maxima.’ Plants, 9(3), 1–13. https://doi.org/10.3390/plants9030346

Das, P., & Srivastav, A. K. (2015). To Study the Effect of Activated Charcoal, Ascorbic Acid and Light Duration on InVitro Micropropagation of Aloe vera L. International Journal of Innovative Research in Science, Engineering and Technology, 4(5), 3131–3138. https://doi.org/10.15680/IJIRSET.2015.0405091

Dolonseda, A. C., Restiani, R., & Aditiyarini, D. (2021). The Use Of Ascorbic Acid And Active Charcoal As Anti-Browning In In Vitro Culture Initiation Of Petung Bamboo (Dendrocalamus asper (Schult.).Biotika,19(2),1–10. 10.24198/biotika.v19i2.34733

Ginting, N. B. O. P., Restiani, R., & Prasetyaningsih, A. (2023). Effect of Ascorbic Acid, Activated Charcoal and Dark Incubation on Browning Intensity of Saurauia bracteosa In Vitro Culture. Biosaintifika: Journal of Biology & Biology Education, 15(3), 401–411.

Habibah, N. A., Moeljopawiro, S., Dewi, K., & Indrianto, A. (2016). Flavonoid Production in Callus Cultures from Mesocarp Stelechocarpus burahol. Biosaintifika: Journal of Biology & Biology Education, 8(2), 214. https://doi.org/10.15294/biosaintifika.v8i2.6632

Habibah, N. A., Moeljopawiro, S., Dewi, K., & Indrianto, A. (2017). Flavonoid production, growth and differentiation of Stelechocarpus burahol (Bl.) hook. f. and th. cell suspension culture. Pakistan Journal of Biological Sciences, 20(4), 197–203. https://doi.org/10.3923/pjbs.2017.197.203

Habibah, N. A., Nugrahaningsih, W. H., Ulung Anggraito, Y., Mukhtar, K., Wijayanti, N., Mustafa, F., & Rostriana, Y. (2019). Effect of Growth Regulators on Cell Growth and Flavonoid Production in Cell Culture of Elaecarpus grandiflorus. IOP Conference Series: Earth and Environmental Science, 391(1). https://doi.org/10.1088/1755-1315/391/1/012061

Habibah, N. A., Sumadi, & Ambar, S. (2013). Optimization of Leaf Surface Sterilization and Endophytic Elimination on Burahol. Biosaintifika, 5(2), 95–98.

Handayani, E., Irsyadi, M. B., Alawiyah, R. L. M. N., & Aris, I. (2022). Effect of Explants Sterilization and Plant Growth Regulators on Embryo Culture of Kepel (Stelechocarpus burahol). IOP Conference Series: Earth and Environmental Science, 985(1), 1–8. https://doi.org/10.1088/1755-1315/985/1/012016

Handayani, E., Irsyadi, M. B., Aris, I., Alawiyah, R. L. M. N., Ayuningtias, N., Permatasari, F., & Rineksane, I. A. (2021). Optimasi Sterilisasi Endosperma Kepel (Stelecthocarpus burahol [Bl] Hook F. & Th) Secara In Vitro. BIO-EDU: Jurnal Pendidikan Biologi, 6(2), 113–121. https://doi.org/10.32938/jbe.v6i2.1179

Handayani, E., Nandariyah, Cahyani, V. R., & Parjanto. (2020). Morphological characters of kepel (Stelechocarpus burahol) from Kulon Progo, Yogyakarta, Indonesia. IOP Conference Series: Earth and Environmental Science, 458(1). https://doi.org/10.1088/1755-1315/458/1/012012

Hatmi, R. U., & Widyayanti, S. (2011). Potensi kepel (Stelechocarpus burahol [Blume] Hook.F & Th.) sebagai Sumber Pangan Fungsional. Prosiding Seminar Nasional Sumber Daya Genetik Pertanian, 22, 248–257.

Helena, A., Restiani, R., & Aditiyarini, D. (2022). Optimasi Antioksidan sebagai Penghambat Browning pada Tahap Inisiasi Kultur In Vitro Bambu Petung (Dendrocalamus asper). Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 7(2), 86–93. https://doi.org/10.24002/biota.v7i2.4715

Hutabarat, C. T., Restiani, R., & Prasetyaningsih, A. (2022).Pengaruh Sterilisasi Tunggal dan Kombinasi pada Kultur In Vitro Nodus Kepel (Stelechorcarpus buraholHookF.&Thomson).Metamorfosa,9(2),235–246. https://doi.org/10.24843/metamorfosa.2022.v09.i02.p01

Isnaeni, E., & Habibah, N. . (2014). Efektivitas Skarifikasi dan Suhu Perendaman terhadap Perkecambahan Biji Kepel [Stelechocarpus burahol (Blume) Hook. F & Thompson] secara In Vitro dan Ex Vitro. Jurnal MIPA, 37(2), 105–114.

Jakhar, M. L., Verma, R., & Dixit, D. (2019). Effect of antioxidants on in vitro degree of browning and culture establishment of Guggul [Commiphora wightii (Arnott )]: A valuable desert medicinal plant. Journal of Pharmacognosy and Phytochemistry, 5, 250–254.

Kumar, R., & Jakhar, M. L. (2018). Factor affecting tissue culture experiments of woody plant: a review. Journal of Cell and Tissue Research, 18(2), 6467–6471.

Rofiqah, U., Ananda, D., & Nurrahmah, I. (2021). The Encapsulation Process of Flavonoid from Kepel Seeds (Stelechocarpus burahol). EKSAKTA: Journal of Sciences and Data Analysis, 2(2), 155–161. https://doi.org/10.20885/eksakta.vol2.iss2.art9

Sekar, A. A., Restiani, R., & Prasetyaningsih, A. (2023). Induksi Kalus dari Eksplan Nodus Stelecocharpus burahol (Blume) Hook. F & Thomson sebagai Upaya Konservasi In Vitro. Biotika, 21(5), 27–35. https://doi.org/10.24198/biotika.v21i1.42869

Sunarni, T., Leviana, F., Fidrianny, I., Immaculata, M., & Wirasutisna, K. R. (2016). Antihyperuricemic and xanthine oxidase inhibitory activities of fractions from ethanolic leaves extract of Stelechocarpus burahol. Asian Journal of Pharmaceutical and Clinical Research, 9(6), 255–258. https://doi.org/10.22159/ajpcr.2016.v9i6.14314

Suparmi, S., Isradji, I., Yusuf, I., Fatmawati, D., Hapsari Ratnaningrum, I., Fuadiyah, S., Indra Wahyuni1, I., & Amelia Rahmah, D. (2015). Anti-Implantation Activity of Kepel (Stelechocarpus burahol) Pulp Ethanol Extract in Female Mice. The Journal of Pure and Applied Chemistry Research, 4(3), 94–99. https://doi.org/10.21776/ub.jpacr.2015.004.03.220

Tarampak, T. C., Sulistiawati, & Nirmala, R. (2019). Metode Mengatasi Browning pada Eksplan Ulin (Eusideroxylon zwageri) untuk Inisiasi Regenerasi secara In Vitro. Jurnal Agroekoteknologi Tropika Lembab Volume, 1(2), 106–117.




DOI: https://doi.org/10.36987/jpbn.v10i1.5464

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