Molecular Identification of Fungi carried by Chili Seeds (Capsicum annuum L.) from Tanjung Morawa B Village, North Sumatra

Dewi Novina Sukapiring, Lily Novianty, Siti Nurlani Harahap

Abstract


Seed-borne fungi are fungi that are associated with seeds and can be a source of plant disease in the field. Therefore, this study aims to utilize DNA barcoding to identify seed-borne fungi isolates from Tanjung Morawa B village. This investigation took place at Tangerang's genetic science laboratory in Tangerang was the site of this investigation. Sequence and phylogenic tree building, PCR amplification with MyTaq HS Red Mix, 2X (Bioline, BIO-25048), and genomic DNA extraction with the Quick-DNA Magbead Plus Kit (Zymo Research, D4082) were the research methods used. The electrophoresis results showed that the DNA made from PCR using Primers ITS-1 and ITS-4 was of high quality. This was clear from the electrophorograms for both the TMIE isolate sample and the control. Using ITS-1 and ITS-4 for DNA amplification produced favorable results, with virtually no smears detected. The Basic Local Alignment and Search Tool (BLAST) program, which is connected to the Genebank database, was employed to analyze the sequence results in the form of a base sequence with a length of 581 base pairs. Based on the genetic similarity results of the isolate's DNA with Genebank, we determined that the TMIE isolate was 100% similar to Curvularia eragrostidis.


Keywords


cendawan terbawa benih, Aspergillus tamarii, Curvularia eragrostidis

References


Alwakeel, S. S. (2017). Molecular identification of fungi isolated from coastal regions of Red Sea, Jeddah, Saudi Arabia. Journal of the Association of Arab Universities, 24(1), 115-119. doi:10.1016/j.jaubas.2016.10.001

An, Y. N., Murugesan, C., Choi, H., Kim, K. D., & Chun, S.-C. (2023). Current Studies on Bakanae Disease in Rice: Host Range, Molecular Identification, and Disease Management. Mycobiology, 51(4), 195-209. doi:10.1080/12298093.2023.2241247

Aslam, S., Tahir, A., Aslam, M. F., Alam, M. W., Shedayi, A. A., & Sadia, S. (2017). Recent advances in molecular techniques for the identification of phytopathogenic fungi – a mini review. Journal Of Plant Interactions, 12(1), 493-504. doi:10.1080/17429145.2017.1397205

Azis, A., & Utoya, B. (2014). Uji Efektivitas Beberapa Jenis Fungisida Terhadap Penyakit Bercak Daun (Curvularia eragrostidis) Pada Bibit Kelapa Sawit di Main-Nursery. Prosiding Seminar Nasional Pengembangan Teknologi Pertanian Polinela 2014 (pp. 231-236). Lampung: Politeknik Negeri Lampung.

Dharmaputra, O. S., Listiyowati, S., & Nurwulansari, I. Z. (2018). Keragaman Cendawan Pascapanen pada Umbi Bawang Merah Varietas Bima Brebes. J Fitopatol Indones, 14(5), 175-182. doi:10.14692/jfi.14.5.175

Elita, N., Susila, E., Agustamar, & Rizki. (2022). Identifikasi Molekuler Trichoderma spp. Indigenous dari Rizosfer Beberapa Varietas Padi Asal Kabupaten Lima Puluh Kota dan Kota Payakumbuh. Agroteknika, 5(1), 1-13. doi:10.32530/agroteknika.v5i1.114

Feau, N., Ramsfiels, T. D., myrholm, C. L., Tomm, B., Cerezke, H. F., Benowicz, A., . . . Hamelin, R. C. (2021). DNA-barcoding identification of Dothistroma septosporum on Pinus contorta var. latifolia, P. banksiana and their hybrid in northern Alberta, Canada. Can. J. Plant Pathol, 43(3), 472–479. doi:10.1080/07060661.2020.1829065

Febriani, A. V., & Kasiamdari, R. S. (2023). Identification of Curvularia eragrostidis (Henn.) J.A.Mey. The Leaf Spot Pathogen of Oil Palm (Elaeis guineensis Jacq.) and Its Control by False Elder (Peronema canescens Jack) Leaf Extract. Int J Oil Palm, 6(2), 25-34. doi:10.35876/ijop.v6i2.111

Gupta, S., Dubey, A., & Singh, T. (2017). Curvularia lunata as, a dominant seed-borne pathogen in Dalbergia sissoo Roxb: Its location in seed and its phytopathological effects. Afr. J. Plant Sci., 11(6), 203-208. doi:10.5897/AJPS2017.1529

ISTA. (2023). International Rules for Seed Testing 2023. Switzerland: The International Seed Testing Association (ISTA). doi:10.15258/istarules.2023.07

Jati, W. W., Kristini, A., Mulyatni, A. S., & Abadi, A. L. (2023). Identifikasi Molekuler Trichoderma sp. Isolat T10 ISRI sebagai Agen Hayati Penyakit Busuk Akar dan Pangkal Batang Xylaria. Indonesian Sugar Research Journal, 3(1), 25-32. doi:10.54256/isrj.v3i1.94

Larasati, S. J., Sabdono, A., & Sibero, M. T. (2021). Identifikasi Molekuler Kapang Asosiasi Spons menggunakan Metode DNA Barcoding. Journal of Marine Research, 10(1), 48-54. doi:10.14710/jmr.v10i1.28334

Mulyani, R. B., Surawijaya, P., Hairani, M., Djaya, A. A., & Pandriyani. (2023). Deteksi Dan Identifikasi Jamur Patogen Terbawa Benih Varietas Padi Lokal Di Kabupaten Kapuas. Jurnal Agri Peat, 24(1), 9-17. doi:10.36873/agp.v24i1.5580

Nontji, M., Muis, A., & A, D. F. (2022). Identifikasi Molekuler Menggunakan Gen 16S-rNA dan Seleksi Limbah Organik sebagai Carrier Terhadap Rhizobakteri Indigenous Asal Rhizosfer Padi Sawah. Seminar Nasional dalam Rangka Dies Natalis ke-46 UNS Tahun 2022, 6, pp. 983-993.

Novaldi, A. L., Dewi, D. K., Ulpa, L. N., Apriyani, S., Hapida, Y., Yuniar, . . . Maretha, D. E. (2018). Review: Isolasi, Identifikasi Molekuler Fungi Endofit Serta Potensinya Sebagi Sumber Bahan Baku. Prosiding Seminar Nasional Pendidikan Biologi 2018, 6-15. Palembang: UIN Raden Fatah Palembang. Retrieved from http://proceedings.radenfatah.ac.id/index.php/semnaspbio

Prihatini, I., Faradilla, F. A., & Suranto. (2019). Teknik Pcr Its Rflp Untuk Seleksi Isolat Jamur Pada Pengujian Agen Pengendali Hayati Pada Serangan Ganoderma Tanaman Acacia mangium. Jurnal Pemuliaan Tanaman Hutan, 13(1), 33-43.

Raja, H. A., Miller, A. N., Pearce, C. J., & Oberlies, N. H. (2017). Review: Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community. J. Nat. Prod, 80, 756-770. doi:10.1021/acs.jnatprod.6b01085

Ramdan, E. P., & Kalsum, U. (2017). Inventarisasi Cendawan Terbawa Benih Padi, Kedelai, dan Cabai. Jurnal Pertanian Presisi , 1(1), 48-58.

Sandy, Y. A., Djauhari, S., & Sektiono, A. W. (2015). Identifikasi Molekuler Jamur Antagonis Trichoderma harzianum Diisolasi Dari Tanah Pertanian Di Malang, Jawa Timur. Jurnal HPT, 3(3), 1-8.

Sari, W., & Rosmeita, C. N. (2019, Juni). Identifikasi Molekuler Cendawan Entomopatogen Beauveria bassiana Dan Metarhizium anisopliae Asal Isolat Cianjur. Jurnal Pro-Stek, 1(1), 1-9.

Sukapiring, D. N. (2024). Inventarisasi Jamur Terbawa Benih Cabai Dari Desa Sidodadi Ramunia, Deli Serdang Sumatera Utara. Konservasi Hayati, 20(1), 34-40. doi:10.33369/hayati.v20i1.33111

Sukapiring, D. N., Novianty, L., & Harahap, S. N. (2023). Inventory of Pathogenic Fungi by Red Chili Seeds (Capsicum annuum L.) from Deli Serdang Regency, North Sumatra. Jurnal Pembelajaran dan Biologi Nukleus, 9(3), 528-534. doi:10.36987/jpbn.v9i3.4894

Ulfa, M., Faridah, E., Sumardi, See, L. S., Mansor, P., Roux, C. l., & Galiana, A. (2019). Identifikasi Molekuler Jenis-Jenis Jamur Mikoriza Ekto Yang Berasosiasi Dengan Dipterokarpa Di Hutan Hujan Tropika Sekunder. Jurnal Wana Tropika, 9(1), 1-14.

Wakhidah, N., Kasrina, & Bustamam, H. (2021). Keanekaragaman Jamur Patogen Dan Gejala Yang Ditimbulkan Pada Tanaman Cabai Merah (Capsicum annuum L.) Di Dataran Rendah. Konservasi Hayati, 17(2), 63-68. doi:10.33369/hayati.v17i2.17920

Waliha, L., Pamekas, T., & Zahara, N. (2022). Aplikasi Ekstrak Kulit Lidah Buaya (Aloe vera L.) untuk Mengendalikan Cendawan Terbawa Benih Padi. Agropross National Conference Proceedings of Agriculture (pp. 375-388). Jember: Agropross National Conference Proceedings of Agriculture. doi:10.25047/agropross.2022.308

Zahara, N., & Pamekas, T. (2022). Karakteristik Cendawan Terbawa Benih Padi Asal Kota Bengkulu. Cermin : Jurnal Penelitian, 6(1), 78-85.




DOI: https://doi.org/10.36987/jpbn.v10i2.5626

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 Dewi N. Sukapiring; Lily Novianty; Siti Nurlani Harahap

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Jurnal Pembelajaran dan Biologi Nukleus (JPBN) by LPPM Universitas Labuhanbatu is under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY - SA 4.0). Official contact: +6281362238917