Formulation Cream of Patikan Kebo Weeds (Euphorbia hirta L.) Extract as an In Vitro Anti-Acne

Lydia Br Barus, Sanna Kamisna Royani Purba, Sesilia Sri Susandri Br. Gultom, Helen Anjelina Simanjuntak, Hermawan Purba, Nurbaiti Br. Singarimbun, Defacto Firmawati Zega

Abstract


Background: Acne is a skin inflammation that is prevalent in 80-100% of the population, particularly during adolescence.  The Patikan Kebo (Euphorbia hirta L.) weed plant is employed in a cream formulation to treat acne. Therefore, this study aims to determine the formula of the Patikan Kebo (E. hirta L.) weed cream preparation that is as effective as an in vitro anti-acne. Methodology: the research method was carried out experimentally with the stages of sample preparation, phytochemical screening, extraction, formulation, evaluation, and antibacterial activity test that caused acne using the well diffusion method. Findings: The results showed the Patikan Kebo (E. hirta L.) weed has secondary metabolites consisting of alkaloids, flavonoids, saponins, tannins, and steroids/triterpenoids. The evaluation of the cream preparation of each formula has met cosmetic standards. The diameter of the F4 inhibition zone has antibacterial activity against acne-causing Propionibacterium acnes (12.03 ± 0.81 mm), Staphylococcus epidermidis (11.48 ± 1.10 mm), S. aureus (12.07 ± 0.06 mm), and the F5 inhibition zone P. acnes (13.53 ± 0.15 mm), S. epidermidis (12.54 ± 0.75 mm), and S. aureus (13.03 ± 0.61 mm). Formulas F4 and F5 are more effective as anti-acne with a strong inhibition zone diameter. Contribution: These findings indicate that formulas F4 and F5 can be further developed as alternative natural active ingredients in cosmetic or pharmaceutical products for acne therapy, thus contributing to the development of safer, more economical, and more sustainable local herbal medicine


Keywords


Anti-Acne, Cream, Euphorbia hirta L., In Vitro, Weed

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References


Adejokun, D. A., & Dodou, K. (2020). Quantitative sensory interpretation of rheological parameters of a cream formulation. Cosmetics, 7(1). https://doi.org/10.3390/cosmetics7010002

Ahmad, W., Singh, S., Kumar, S., & Waseem Ahmad, C. (2017). Phytochemical Screening and antimicrobial study of Euphorbia hirta extracts. ~ 183 ~ Journal of Medicinal Plants Studies, 5(2), 183–186.

Azmalah, A., & Firianingsih, S.P. (2023). Antibacterial Activity Test of Red Galangal Leaf Extract Against Acne-Causing Bacteria. Jurnal Riset Farmasi, 3(1), 17–22. https://doi.org/10.29313/jrf.v3i1.1808 [In Indonesian language]

Barus, L. B., Manihuruk, F. N., Sari, M. P., Simanjuntak, H. A., Ginting, J. G., & Purba, H. (2023). The Utilisation of Coconut Water Waste into Nanocellulose and Potential Synergistic Effect of Asthma Plant (Euphorbia hirta L.) Extract as an Antibacterial Agent. Jurnal Pembelajaran Dan Biologi Nukleus, 9(3), 688–695. https://doi.org/10.36987/jpbn.v9i3.5060

Basma, A. A., Zakaria, Z., Latha, L. Y., & Sasidharan, S. (2011). Antioxidant activity and phytochemical screening of the methanol extracts of Euphorbia hirta L. Asian Pacific Journal of Tropical Medicine, 4(5), 386–390. https://doi.org/10.1016/S1995-7645(11)60109-0

Basyal, D., Neupane, A., Pandey, D. P., & Pandeya, S. (2021). Phytochemical Screening and In Vitro Antioxidant and Anti-inflammatory Activities of Aerial Parts of Euphorbia hirta L. Journal of Nepal Chemical Society, 42(1), 115–124. https://doi.org/10.3126/jncs.v42i1.35362

Davis, W. W., & Stout, T. R. (1971). Disc plate method of microbiological antibiotic assay. I. Factors influencing variability and error. Applied Microbiology, 22(4), 659–665. https://doi.org/10.1128/aem.22.4.659-665.1971

Dudhe, A. R., Choudhari, N., Dudhe, R., Katole, G., Mahajan, R., Pathak, N., Darode, A., Devnani, D., & Rd, H. (2023). Formulation and Evaluation of Multipurpose Herbal Cream. International Journal of Newgen Research in Pharmacy & Healthcare. 1, 129–134.

Evelia, K., Sitanggang, P., Zega, N. P., Anggreni, W., Manurung, B., Cytra, E., Silaban, B., & Simanjuntak, H. A. (2024). Clay Mask Formulation of Coconut Dregs and Virgin Coconut Oil as an Antibacterial Against Propionibacterium acnes. Jurnal Pembelajaran dan Biologi Nukleus, 10(3), 809–819.

Franco-Gil, M. E., Graça, A., Martins, A., Marto, J., & Ribeiro, H. M. (2024). Emollients in dermatological creams: Early evaluation for tailoring formulation and therapeutic performance. International Journal of Pharmaceutics, 653(December 2023), 123825. https://doi.org/10.1016/j.ijpharm.2024.123825

Gani, F. A., Isnaini, N., & Maryam, S. (2020). Formulation and Investigation Antioxidant of O/W Cream Containing Euphorbia hirta L. Herb Extract. E3S Web of Conferences, 151, 1–4. https://doi.org/10.1051/e3sconf/202015101001

GI, U., Oili, AN., Igbokwe, NH., Ohwoavworhua, FO., Ojiako, CM., Obaji, MU., & Esimone, CO. (2022). Formulation and Pharmaceutical Characterization of Antidermatophyte Creams of Methanol Leaf Extracts of Euphorbia Hirta, Jatropha Curcas and Acantus Montanus. Journal of Infectious Diseases & Travel Medicine, 6(1), 000157

Ginting, J. G., Br. Singarimbun, N., Daeli, M. C. Y., Simanjuntak, H. A., Purba, H., Br. Barus, L., & Zega, D. F. (2023). Inhibition Test of Ethanol from Extract Mangosteen Leaves (Garcinia mangostana L.) as an Acne Antibacterial. Jurnal Pembelajaran Dan Biologi Nukleus, 9(3), 659–667. https://doi.org/10.36987/jpbn.v9i3.5066

Johnson, C. C., Etim, E. I., Udobre, A. S., & Imeuka, T. I. (2018). Qualitative and Quantitative Evaluation of Phytochemical Constituents of Ageratum conyzoides L. (Asteraceae). Nigerian Journal of Pharmaceutical and Applied Science Research, 7(3), 54–58.

Khan, S. A., Ahmad, F., Akram, M. Z., Tongyu, Y., Urooj, F., Ullah, S., Zulfiqar, S., Batool, T., Sarwar, A., & Sultan, Y. (2024). Research Article Morpho-Anatomical Alterations in Asthama Plant (. 40(4), 1144–1152.

Kumar, B., Pathak, R., Mary, P. B., Jha, D., Sardana, K., & Gautam, H. K. (2016). New insights into acne pathogenesis: Exploring the role of acne-associated microbial populations. Dermatologica Sinica, 34(2), 67–73. https://doi.org/10.1016/j.dsi.2015.12.004

Kurniadi, M. L., Kawiyanto, J., Mangunwijaya, P. K., & Kefarmasian, P. (2024). Acne Cream Formula from Chinese Betel Leaf Extract (Peperomia pellucida L.). Jurnal Ilmiah Farmasi Terapan & Kesehatan . 2(2), 31–38. [In Indonesian language]

Leccia, M. T., Auffret, N., Poli, F., Claudel, J. P., Corvec, S., & Dreno, B. (2015). Topical acne treatments in Europe and the issue of antimicrobial resistance. Journal of the European Academy of Dermatology and Venereology, 29(8), 1485–1492. https://doi.org/10.1111/jdv.12989

Lisa Vironika Agustin Ningtias, & Luluk Aniqoh M.P. (2024). Antibacterial Test Ointment Patikan Kebo Leaf Extract (Euphorbia hirta L.) Against Staphylococcus epidermidis Bacteria Using Diffusion Method. Strada Journal of Pharmacy, 6(1), 14–25. https://doi.org/10.30994/sjp.v6i1.108

Murdiana, H. E., Putri, M. K., Rosita, M. E., Kristariyanto, Y. A., & Kurniawaty, A. Y. (2022). Optimisation of Rice Cream (Oryza Sativa L.) Formula Type M/a with Variations in Stearic Acid, Cetyl Alcohol and Triethanolamine. Jurnal Farmamedika (Pharmamedica Journal), 7(2), 55–63. https://doi.org/10.47219/ath.v7i2.161 [In Indonesian language]

Pariury, J. A., Juan PCH., Tiffany R., Elvina V., & I Gusti Kamasan Nyoman Arijana. (2021). The Potential of Bali Orange Peel (Citrus maxima Merr) as an Antibacterial Agent against Propionibacterium acnes, the Cause of Acne. Hang Tuah Medical Journal, 19(1), 119–131. https://doi.org/10.30649/htmj.v19i1.65 [In Indonesian language]

Prajakta, S., & Shahu, K. (1970). Formulation and Evaluation of Vanishing Herbal Cream of Crude Drugs. Asian Journal of Pharmaceutical Research and Development, 8(3), 66–69. https://doi.org/10.22270/ajprd.v8i3.725

Pramod, P., Ravindra, B., Ashok, V., Nihalsing, R., Chotulal, N., & Pannalal, P. (2020). Formulation and Development of Anti-Acne Serum Using Euphorbia Hirta. International Journal Of Creative and Innovative Research In All Studies (IJCIRAS), 2(12), 171–179. http://www.ijciras.com/PublishedPaper/IJCIRAS1587.pdf

Praveen, G., Krishnamoorthy, K., Veeraraghavan, V. P., & Jayaraman, S. (2024). Antioxidant and Anti-Inflammatory Activity of the Ethanolic Extract of Euphorbia Hirta Leaf Extract: An In Vitro and In Silico Study. Journal of Pharmacy and Bioallied Sciences, 16(Suppl 2), S1304-S1307.

Rafique, M., & Hussain Shah, S. N. (2019). Anti-Ageing Potential of a Cream (W/O Emulsion) Containing Grape Seed Extract (GSE): Formulation and in vivo Evaluation of Effectiveness Using Non-Invasive Biophysical Technique. Journal of Clinical & Experimental Dermatology Research, 10(4). https://doi.org/10.35248/2155-9554.19.10.500

Roosevelt, A., H. Ambo Lau, S., & Syawal, H. (2019). Formulation and Stability Testing of Methanol Extract Cream from Beluntas Leaves (Pluchea indica L.) from Benteng City, Selayar Islands Regency, South Sulawesi Province. Jurnal Farmasi Sandi Karsa, 5(1), 19–25. https://doi.org/10.36060/jfs.v5i1.36 [In Indonesian language]

Ruchiatan, K., Rahardja, J. I., Rezano, A., Hindritiani, R., Sutedja, E., & Gunawan, H. (2020). A five-year clinical acne patients profiles and its management based on Indonesian acne expert guideline in Bandung, Indonesia. Journal of Pakistan Association of Dermatologists, 30(2), 229–234.

Safitri M., Mohammad Z., Ery E. (2016). Development of Formulation and Physical Evaluation of 70% Ethanol Extract Cream from Chayote Leaves (Sechium edule (Jacq.) Swatz). Farmagazine, 3(2), 7. [In Indonesian language]

Satpute, K. L., & Kalyankar, T. M. (2019). Development and evaluation of herbal cream for the treatment of acne. Journal of Pharmacognosy and Phytochemistry, 8(3), 2618–2624.

Simanjuntak, H. A., & Rahmiati, R. (2021). Antibacterial and Antifungal Activities of Patikan Kebo (Euphorbia hirta L.) Herb Ethanol Extract. Asian Journal of Pharmaceutical Research and Development, 9(5), 6–9. https://doi.org/10.22270/ajprd.v9i5.1017

Tan, P. L., Rajagopal, M., Chinnappan, S., Selvaraja, M., Leong, M. Y., Tan, L. F., & Yap, V. L. (2022). Formulation and Physicochemical Evaluation of Green Cosmeceutical Herbal Face Cream Containing Standardized Mangosteen Peel Extract. Cosmetics, 9(3). https://doi.org/10.3390/cosmetics9030046

Tungadi, R., Sy. Pakaya, M., & D.as’ali, P. W. (2023). Formulation and Evaluation of Physical Stability of Astaxanthin Compound Cream Preparations. Indonesian Journal of Pharmaceutical Education, 3(1), 117–124. https://doi.org/10.37311/ijpe.v3i1.14612 [In Indonesian language]




DOI: https://doi.org/10.36987/jpbn.v11i2.6698

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