Characteristic Isolation of Bacteria in Lake Water on Campus-A of UIN Sulthan Thaha Saifuddin Jambi
Abstract
Background: Water bodies are highly susceptible to contamination from various sources, including domestic, agricultural, and industrial waste. These contaminants often introduce pathogenic microorganisms such as Escherichia coli and Salmonella sp., which pose significant risks to human and animal health. Despite this, bacteria also play a vital role in supporting aquatic ecosystems by facilitating the decomposition of organic matter. Methodology: This study was conducted with the objective of isolating and characterizing bacterial species present in the waters of Campus A Lake. Sampling was carried out by collecting water from several points within the lake. Bacterial isolation was performed using the serial dilution technique, followed by inoculation on Nutrient Agar (NA) media and incubation at 37 °C for 24 hours. The characterization phase included morphological observation of colonies, Gram staining, and biochemical tests such as oxidase reactions. Findings: The study successfully isolated three distinct bacterial colonies. One isolate exhibited a rod-shaped (bacillus) morphology, while the remaining two showed a spherical (coccus) form. All isolates tested Gram-negative, evidenced by the pink coloration after staining. Importantly, most of the isolates demonstrated an ability to break down organic material, indicating their usefulness in environmental cleanup. Additionally, the introduction of Eco Enzyme into lake water positively influenced the microbial ecosystem by promoting the growth of beneficial decomposers like Bacillus sp. and Lactobacillus sp., while simultaneously suppressing pathogenic bacteria. Contribution: These findings provide valuable insight for future water quality management and the development of effective, eco-friendly bioremediation strategies.
Keywords
Full Text:
PDFReferences
Anderson, T., & Smith, P. (2019). Techniques for Isolating Aquatic Microorganisms. Journal of Environmental Microbiology, 15(2), 123-135.
Bi, S., & Zhang, Y. J. (2018). Computation rate maximization for wireless powered mobile-edge computing with binary computation offloading. IEEE Transactions on Wireless Communications , 17 (6), 4177-4190.
Bunyamin, MS (2020). Effectiveness Of Clean Water Distribution Of PDAM Tirta Daroy In Banda Aceh City (Case Study of Syiah Kuala and Teuku Nyak Arief Branches). Journal of Research Science , 10 (1), 35-44.
Cappuccino, J. G., & Welsh, C. (2019). Microbiology: A Laboratory Manual. 12th edition. Boston, New York: Pearson.
Ely, R., Thompson, A., Nguyen, L., & Patel, S. (2017). Gram Staining as a Diagnostic Tool in Microbiology. Journal of Microbial Techniques, 28(4), 89-95.
Febriza, A., Zahrah, AA, Andini, NS, Usman, F., & Idrus, HH (2024). Potential Effect of Curcumin in Lowering Blood Glucose Levels in Streptozotocin-Induced Diabetic Rats. Diabetes, metabolic syndrome and obesity : targets and therapy, 17, 3305–3313. https://doi.org/10.2147/DMSO.S468059.
He, F., Lin, J., & Zheng, X. (2019). Nutrient Dynamics in Polluted Lakes and Implications for Restoration. Water Resources Management, 20(3), 45-60.
He, Xinyue, Liu, Yuguang, Li, Yan, Zhao, Jun, Wang, Yue, & Dong, Zhijun. (2022). Temporal and spatial characteristics of harmful algal blooms in the offshore waters, China during 1990 to 2019. Journal of Applied Remote Sensing 16.1: 012004-012004.
Jeppesen, Erik, Meerhoff, Mariana, Davidson, Thomas A., Trolle, Dennis, Søndergaard, Martin, Lauridsen, Torben L., Beklioğlu, Meryem, Brucet, Sandra, Volta, Pietro, Guilizzoni, Paolo, Simonato, Mauro, Martinez, Sònia, & Liu, Zhengwen. (2015). “Ecological impacts of global warming and water abstraction on lakes and reservoirs due to changes in water level and related changes in salinity”. Hydrobiologia, 750, 201-227.
Laila, R., Park, JI, Robin, AHK, Natarajan, S., Vijayakumar, H., Shirasawa, K., ... & Nou, IS (2019). Mapping of a novel clubroot resistance QTL using ddRAD-seq in Chinese cabbage (Brassica rapa L.). BMC plant biology, 19 , 1-9.
Le, TM, Nguyen, HMN, Nguyen, VK, Nguyen, AV, Vu, ND, Yen, NTH, ... & Tran, TM (2021). Profiles of phthalic acid esters (PAEs) in bottled water, tap water, lake water, and wastewater samples collected from Hanoi, Vietnam. Science of the Total Environment , 788 , 147831.
Lin, G., Xu, X., Wang, P., Liang, S., Li, Y., Su, Y., ... & Wang, X. (2020). Methodology for forecast and control of coastal harmful algal blooms by embedding a compound eutrophication index into the ecological risk index. Science of the Total Environment , 735 , 139404.
Liu, Y., Zhang, H., Chen, X., & Wang, L. (2018). Eco Enzyme as a Natural Solution for Water Quality Improvement. Journal of Applied Biotechnology, 27(6), 98-112.
Ma, R., Li, J., Zhou, T., & Huang, Y. (2022). Role of Bacteria in Biogeochemical Processes of Aquatic Environments. Aquatic Microbial Ecology, 89(2), 145-160.
Madigan, Michael T., Bender, Kelly S., Buckley, Daniel H., Stahl, David A., & Martenko, Thomas. (2018). Brock Biology of Microorganisms. 15th edition. New York: Pearson.
Martinez, M., Johnson, R. T., & Lee, S. H. (2018). The Utility of Gram Staining in Environmental Microbiology. Microbial Ecology Reports, 32(1), 33-42.
Pan, J. (2020). Influence of Eco Enzymes on Microbial Communities in Lakes. Journal of Ecological Chemistry, 12(1), 34-41.
Randle, S., & Barnes, J. (2018). Liquid futures: Water management systems and anticipated environments. Wiley Interdisciplinary Reviews: Water, 5 (2), e1274.
Sumampouw, O. (2017). Toddler Diarrhea: A Review from the Field of Public Health. Yogyakarta: Deepublish.
Sumarmono, J., & Wijayanto, S. (2019). Techniques for Bacterial Morphology Observation. Environmental Microbial Research, 29(3), 210-218.
Tortora, G. J, Funke, B.R., Case, C.L. (2020). Microbiology: An Introduction. 13th edition. Boston: Pearson.
Widyaningsih, W., Supriharyono, S., & Widyorini, N. (2016). Analysis of total coliform bacteria in the waters of the Wiso Jepara estuary. Management of Aquatic Resources Journal (Maquares), 5 (3), 157-164.
Wu, Q., Li, J., Chen, Y., Wang, H., & Zhou, L. (2020). The Role of Bacteria in Carbon and Nitrogen Cycling in Freshwater Systems. Aquatic Science Journal, 67(5), 78-90.
Zhang, X., Liu, M., Yang, D., Chen, F., & Sun, H. (2021). Nutrient Cycling Mediated by Microbial Communities in Lakes. Journal of Microbial Ecology, 34(3), 102-118.
Zhao, Q., Wang, X., Lin, Y., Ma, J., & Tang, S. (2020). Pathogenic Bacteria and Water Quality Challenges in Freshwater Lakes. Water Research, 66(5), 210-225.
Zheng, S., Wang, R., Kainz, M.J., Liu, C., Li, P., Li, Z., ... & Yin, D. (2022). How phytoplankton biomass controls metal (loid) bioaccumulation in size-fractionated plankton in anthropogenic-impacted subtropical lakes: A comprehensive study in the Yangtze River Delta, China. Water Research, 224 , 119075.
DOI: https://doi.org/10.36987/jpbn.v11i2.6846
Refbacks
- There are currently no refbacks.
Copyright (c) 2025 Latusi Anggriani, Aaini Qomariah Manurung, Andina Dwiana Marsa, Nisma Hariyanti Ritonga, Cheryska Alinza, Nuraisyah Gustri Anggrai Ningsih, Muhammad Arif Januarda Saputra

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Pembelajaran dan Biologi Nukleus (JPBN) by LPPM Universitas Labuhanbatu is under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY - SA 4.0). Official contact: Rivo +6281362238917