The Cellulase Enzyme Activity of Thermophilic Bacteria from Way Belerang Hot Spring, Lampung

Rismawati Hidayah, Erma Suryanti, Meezan Ardhanu Asagabaldan, Budhi Agung Prasetyo

Abstract


Background: The demand for cellulase enzymes across various industries continues to rise, while most of the current supply still depends on imports. Therefore, exploring local biological resources, such as thermophilic bacteria capable of producing cellulase, is crucial. This study aims to isolate and evaluate the cellulase enzyme activity of thermophilic bacteria from the Way Belerang Hot Spring in South Lampung, Indonesia. Methodology: The research involved isolating bacteria from hot spring water samples, conducting qualitative and quantitative assays of cellulase activity, and characterizing the isolates. Findings: A total of 24 bacterial isolates were obtained, 16 of which demonstrated cellulolytic activity, as indicated by clear zones on 1% CMC agar. The two most promising isolates, S5.1 and S5.24, exhibited the highest cellulolytic indices of 2.728 and 2.395, respectively. These isolates were further tested quantitatively using a glucose standard curve to determine their enzyme activity. The highest cellulase activity for isolate S5.1 was recorded at the 10th hour with 5.1 × 10⁻² U/mL, while isolate S5.24 showed peak activity at the 14th hour with 3.1 × 10⁻² U/mL. Contribution: This study represents the first investigation of thermophilic amylase-producing bacteria from the Way Belerang Hot Spring, highlighting the potential of thermostable amylase enzymes that can withstand high temperatures for future industrial applications.


Keywords


Cellulase Enzyme; Cellulolytic Index; Enzyme Activity; Thermophilic bacteria; Way Belerang

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References


Ajeje, S. B., Hu, Y., Song, G., Peter, S. B., Afful, R. G., Sun, F., Asadollahi, M. A., Amiri, H., Abdulkhani, A., & Sun, H. (2021). Thermostable cellulases/xylanases from thermophilic and hyperthermophilic microorganisms: Current perspective. Frontiers in Bioengineering and Biotechnology, 9, 794304.

Amin, S. S., Ghozali, Z., Rusdiana, M., & Efendi, S. (2023). Identifikasi Bakteri dari Telapak Tangan dengan Pewarnaan Gram Identification of Bacteria from Palms with Gram Stain. CHEMVIRO: JurnalKimiadanIlmuLingkungan.

Apriliani, D., & Enny, Z. (2021). Viability and Production Calcifying Bacterial Endospore on Sand-Cement Carrier. Jurnal Ilmiah Biologi Eksperimen Dan Keanekaragaman Hayati (J-BEKH), 8(1), 8–13.

Baharuddin, M., Khaerah, N., Fadillah, N., Sappewali, & Azis, F. (2022). Isolasi dan Identifikasi Khamir pada Larva Cossus cossus Penghasil Selulase. Eknosains: Media Informasi Sains Dan Teknologi, 16(3), 291–300.

Baharuddin, M., Patong, A. R., Ahmad, A., & La Nafie, N. (2014). Aktivitas Enzim Selulase Kasar dari Isolat Bakteri Larva Cossus cossus dalam Hidrolisis Jerami Padi. Jurnal Kimia Valensi, 4(2), 128–133.

Ejaz, U., Sohail, M., & Ghanemi, A. (2021). Cellulases: From bioactivity to a variety of industrial applications. Biomimetics, 6(3), 44.

Fauziah, S. I., & Ibrahim, M. (2020). Isolasi dan Karakterisasi Bakteri Selulolitik pada Tanah Gambut di Desa Tagagiri Tama Jaya, Kecamatan Pelangiran, Kabupaten Inhil, Riau. LenteraBio: Berkala Ilmiah Biologi, 9(3), 194–203.

Firman, E. N., Kaskoyo, H., HEerwanti, S., & Bakri, S. (2023). Identification of tourism attraction at Way Belerang In Kalianda Sub-district, South Lampung, Indonesia. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia, 9(1), 75–79. https://smujo.id/psnmbi/article/view/15997

Hamidah, M. N., Rianingsih, L., & Romadhon, R. (2019). Aktivitas antibakteri isolat bakteri asam laktat dari peda dengan jenis ikan berbeda terhadap E. coli dan S. aureus. Jurnal Ilmu Dan Teknologi Perikanan, 1(2), 11–21.

Hanifa, N. I., Deccati, R. F., & Muliasari, H. (2021). Isolasi dan Uji Aktivitas Enzim Selulase dari Rumen Sapi (Bibos javanicus). Journal of Science, Technology and Entrepreneur, 3(1). http://ejournal.umbandung.ac.id/index.php/jste/article/ view/163

Imanisa, T. W., Mardawati, E., & Masruchin, N. (2023). Xylanase Production from Aspergillus niger via Submerged Fermentation towards Oil Palm Empty Fruit Bunches (OPEFB) Valorization as Value-added Biorefinery Products. Biomass, Biorefinery and Bioeconomy, 1(1).

Irdawati, I., Putri, I. S., Syamsuardi, A. A., & Rilda, Y. (2018). The Thermophilic Bacterial Growth Curve. Bioscience, 2(2), 58–64.

Kasi, P. D. (2020). Karakterisasi morfologis isolat bakteri termofilik dari sumber Air Panas Pincara. Indigenous Biologi: Jurnal Pendidikan Dan Sains Biologi, 3(2), 51–56.

Kuhad, R. C., Gupta, R., & Singh, A. (2011). Microbial Cellulases and Their Industrial Applications. Enzyme Research, 2011, 1–10. https://doi.org/ 10.4061/2011/280696

Kurniawati, L., Kusdiyantini, E., & Wijanarka, W. (2019). Pengaruh variasi suhu dan waktu inkubasi terhadap aktivitas enzim selulase dari bakteri Serratia marcescens. Jurnal Akademika Biologi, 8(1), 1–9.

Kusumaningrum, A., Gunam, I. W., & Wijaya, I. M. (2019). Optimasi suhu dan pH terhadap aktivitas enzim endoglukanase menggunakan response surface methodology (RSM). Jurnal Rekayasa Dan Manajemen Agroindustri, 7(2), 243.

Lestari, R. S., Nur, H. S., & Imaningsih, W. (2024). Karakterisasi Selulase Termostabil asal Isolat Bakteri Tanah Pertanian Pasang Surut. BIOSCIENTIAE, 21(1), 41–56.

Lv, A.-P., Ming, Y.-Z., Liu, Z.-T., Ouyang, Y.-T., Li, M.-M., Lian, Z.-H., Jiao, J.-Y., She, T.-T., Liu, L., & Li, W.-J. (2023). Clostridium caldaquaticum sp. Nov., a thermophilic bacterium isolated from a hot spring sediment. International Journal of Systematic and Evolutionary Microbiology, 73(1). https://doi.org/10.1099/ijsem.0.005684

Mahestri, L., Harpeni, E., & Setyawan, A. (2021). Isolasi dan penapisan bakteri termofilik pemecah amilum dan protein dari sumber air panas Way Panas Kalianda Lampung Selatan. Jurnal Perikanan Dan Kelautan, 26(3), 161–168.

Majidah, R., Lubis, S. S., & Harahap, D. (2023). Potensi Enzim Selulase dari Bakteri Termofilik di Kawasan Wisata Ie Suum Kabupaten Aceh Besar. KENANGA: Journal of Biological Sciences and Applied Biology, 3(1), 25–34.

Marina, M., Lambui, O., & Suwastika, I. N. (2018). Karakterisasi Selulase Asal Bakteri Tanah Danau Kalimpa’a Sulawesi Tengah. Natural Science: Journal of Science and Technology, 7(2). https://bestjournal.untad.ac.id/index.php/ejurnal fmipa/article/view/10563

Mawati, S. D., Harpen, E., & Fidyandini, H. P. (2021). Skrining Bakteri Termofilik Potensial Amilolitik Dari Sumber Air Panas Way Belerang Kalianda Lampung Selatan. Journal of Aquatropica Asia, 6(1), 1–7.

Megahati, R. R. P. (2017). Bakteri Termofil Penghasil Selulase Asal Sumber Air Panas di Indonesia. Jurnal Bioconcetta, 3(1), 61–68.

Mukminin, A. (2014). Isolasi bakteri selulolitik termofilik dari sumber air panas pacet Mojokerto dan pengujian aktivitas enzim selulase. [Thesis, Universitas Islam Negeri Maulana Malik Ibrahim]. http://etheses.uin-malang.ac.id/2543/

Mulyasari, M., Melati, I., & Sunarno, M. T. D. (2015). Isolasi, seleksi, dan identifikasi bakteri selulolitik dari rumput laut Turbinaria sp. Dan Sargassum sp. Sebagai kandidat pendegradasi serat kasar pakan ikan. Jurnal Riset Akuakultur, 10(1), 51–60.

Murtiyaningsih, H., & Hazmi, M. (2017). Isolasi dan uji aktivitas enzim selulase pada bakteri selulolitik asal tanah sampah. Agritrop: Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science), 15(2). http://jurnal.unmuhjember.ac.id/ index.php/ AGRITROP/article/view/1185

Nababan, M., Gunam, I. B. W., & Wijaya, I. M. M. (2019). Produksi enzim selulase kasar dari bakteri selulolitik. Jurnal Rekayasa Dan Manajemen Agroindustri, 2503, 488X.

Nafiqoh, N., & Suryaningrum, L. H. (2020). Hidrolisis ampas tebu menggunakan enzim selulase dari bakteri Bacillus subtilis dalam upaya pemanfaatannya sebagai bahan pakan ikan. Prosiding Seminar Nasional Biologi, 6(1), 428–435. https://journal3.uin-alauddin.ac.id/index.php/psb/article/view/16022

Narayan, V. V., Hatha, M. A., Morgan, H. W., & Rao, D. (2008). Isolation and characterization of aerobic thermophilic bacteria from the Savusavu hot springs in Fiji. Microbes and Environments, 23(4), 350–352.

Pratiwi, W. M., & Asri, M. T. (2022). Isolasi dan Identifikasi Bakteri Indigenous Pendegradasi Pestisida Profenofos dan Klorantraniliprol di Jombang Jawa Timur. LenteraBio: Berkala Ilmiah Biologi, 11(2), 300–309.

Risna, Y. K., Sri-Harimurti, S.-H., Wihandoyo, W., & Widodo, W. (2022). Kurva pertumbuhan isolat bakteri asam laktat dari saluran pencernaan itik lokal asal aceh. Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science), 24(1), 1–7.

Sari, U. M., & Agustien, A. (2012). Penapisan dan karakterisasi bakteri selulolitik termofilik sumber air panas Sungai Medang, Kerinci, Jambi. Jurnal Biologi UNAND, 1(2).http://jbioua.fmipa.unand.ac.id/index.php/jbioua/article/ view/28/0

Saropah, D. A., Jannah, A., & Maunatin, A. (2012). Kinetika reaksi enzimatis ekstrak kasar enzim selulase bakteri selulolitik hasil isolasi dari bekatul. ALCHEMY: Journal of Chemistry. http://ejournal.uin-malang.ac.id/index.php/Kimia/ article/view/2297/0

Sonia, N. M. O., & Kusnadi, J. (2016). Isolasi dan Karakterisasi Parsial Enzim Selulase dari Isolat Bakteri OS-16 Asal Padang PAasir Tengger-Bromo. Jurnal Pangan Dan Agroindustri, 4(1). https://jpa.ub.ac.id/index.php/jpa/article/ view/300

Vavitsas, K., Glekas, P. D., & Hatzinikolaou, D. G. (2022). Synthetic biology of thermophiles: Taking bioengineering to the extremes. Applied Microbiology, 2(1), 165–174.

Wardani, A. K., Brahmanti, A. A., & Martati, E. (2023). Leclercia adecarboxylata C12, the newly isolated cellulose-degrading bacteria from Indonesian coffee pulp. HAYATI Journal




DOI: https://doi.org/10.36987/jpbn.v11i3.7493

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