Assessment of Mixed Cultivation Substrate (Burnt Husk-Sawdust-Bagasse) and NPK Fertilizer on Growth Performance of Oyster Mushroom (Pleurotus ostreatus)

Muthmainnah Muthmainnah, M. Idris

Abstract


Background: Oyster mushroom cultivation generally uses sawdust as the primary rowin medium. Limited availability and fluctuating prices require innovation for mushroom planting media. The use of bagasse and burnt husk waste as an alternative medium in an effort to maximize abundant but underutilized agroindustrial resources has the potential to become a medium for growing oyster mushrooms, as the cellulose and lignocellulose content can support optimal mycelium growth. Therefore, this study aims to analyze the effect of using burnt husk as planting media, bagasse, and NPK fertilizer on the growth of oyster mushrooms (Pleurotus ostreatus). Methodology: This study employed a factorial Group Random Design method, consisting of two factors: the combination factor of substrate media (M) and NPK fertilizer dosage (N). The observed parameters included mycelial growth, cap dimeter and fresh weight of the mushroom. Data were analyzed by using Analisys of Variance (ANOVA) with SPSS v.25. Findings: The results of the data analysis showed the optimization of fungal growth in a combination of 1000 gr of sawdust and 80 gr burnt husk without the addition of NPK fertilizer with f calculated > f table (7.08>3.63). The ANOVA test confirmed that NPK fertilizer did not have a significant effect on the quality of mushrooms, possibly even negatively, because NPK creates nutrient-rich conditions that favor competing microorganisms that develop faster than oyster mushrooms. The combination of burnt husk and sawdust without NPK (M1N0) resulted in the highest mycelial growth, reaching 21.3 cm, while the lowest growth was observed in the sawdust and bagasse media with the addition of NPK (15 g) (M2N1), which was 16 cm. Meanwhile, the highest cap diameter was obtained in the Sugarcane bagasse and sawdust media without NPK (M2N0), with an average value of 8 cm. In contrast, the lowest diameter was observed in the bagasse media with an NPK of 20 g (M2N2), which was 5 cm. Contribution: This research confirms that burnt husk and sugarcane bagasse substrates, without NPK fertilizer, can serve as an effective and environmentally friendly alternative medium for oyster mushroom cultivation.


Keywords


Fertilizer; Oyster Mushroom; Planting Medium

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References


Afief, M. F., Lahay, R. R., & Siagian, B. (2015). Growth and Production Response of White Oyster Mushroom (Pleurotus ostreatus) On Various Sawdust Media and NPK Fertilizer Response to Growth and Production of White Oyster Mushroom (Pleurotus ostreatus) To Various Media sawdust and giving NPK Fe. Online Journal of Agroecotechnology, 3(4), 1381–1390. [In Indonesian language]

Azzahra, Yulia Riska, Toto Toharmat, and Iwan Prihantoro. (2022). Evaluation of the Physical Characteristics of Mushroom Fermented Media (Pleurotus ostreotus) as an Alternative Animal Feed for Ruminants. Indonesian Journal of Agricultural Sciences, 27(3), 351–58. https://doi.org/10.18343/jipi.27.3.351. [In Indonesian language]

BPS North Sumatra Province. (2024). North Sumatra Province in 2024 Figures. In BPS North Sumatra Province. https://sumut.bps.go.id/id/publication/2024/02/28/a2b9ed5089227612befc7827/provinsi-sumatera-utara-dalam-angka-2024.html [In Indonesian language]

Bukit Fikri, Andika et al. (2025). Test Several Planting Media Mixtures on the Growth and Production of Oyster Mushroom (Pleurotus Ostreatus). Journal of Agroplasm, 10(1), 170–181. [In Indonesian language]

Choliq Kurniawan, Widodo, I., & Abbas, B. (2021). Growth and Development of White Oyster Mushrooms (Pleurotus ostreatus) in Sago Pulp Media. Cassowary, 4(1), 28–38. https://doi.org/10.30862/casssowary.cs.v5.i1.92 [In Indonesian language]

Criswantara, D. (2021). Effect of applying bagasse and liquid organic fertilizer (POC) of banana peels on the growing medium of oyster mushrooms (pleurotus astreatus). Scientific Journal of Agricultural Students, 1, 1–47. http://jurnalmahasiswa.umsu.ac.id/index.php/jimtani/article/view/941%0A [In Indonesian language]

Devotion, J., Vol, M., & Issn, O. (2021). Training on Making Organic Fertilizer from Baglog Waste to Increase Income in Oyster Mushroom Farmer Groups in Medan Denai Village, Medan Denai District. Ihsan: Journal of Community Service, 3(1), 73–81. https://doi.org/10.30596/ihsan.v3i1.6817

Elfandari, H., Yusanto, & Septiana. (2021). Growth And Productivity Of Oyster Mushroom (Pleurotus Ostreatus) In Media Composition Of Sengon And Straw Plant. Journal of Tropical Agrotechnology, 9(2), 301–305.

Erlinda, C., Prasetyaningsih, A., & Madyaningrana, K. (2021). The Effect of Sugarcane Bagasse Composting as an Alternative Media and Its Effect on the Productivity of White Oyster Mushroom (Pleurotus ostreatus). LenteraBio : Scientific Periodical of Biology, 11(1), 161–173. https://doi.org/10.26740/lenterabio.v11n1.p161-173 [In Indonesian language]

Fajar, R., Siregar, S., Pane, E., & Mardiana, S. (2020). The Test Of Some Oyster Mushroom Varieties On The Media Combination Of Bagasse And Sawdust By Adding Molasses And Tofu Dregs. Scientific Journal of Agriculture (JIPERTA), 1(1), 106–111. http://jurnalmahasiswa.uma.ac.id/index.php/jiperta

Feyrisha Nugrani et al. (2024). Revitalization of Oyster Mushroom Growing Media with Gamal Leaf Liquid Organic Fertilizer. Journal of Agricultural Science and Technology, 4(2), 126–144. [In Indonesian language]

Hapida, Y. (2019). The use of sugarcane bagasse in increasing the growth of white oyster mushrooms (Pleurotus ostreatus) in Palembang City and its contribution to biology subjects in high school. Bioscience: Journal of Education, 5(1), 23–28. https://doi.org/10.19109/bioilmi.v5i1.3508 [In Indonesian language]

Hariri, A. M., Saraswati, K. D., Suskandini Ratih Dirmawati, & Fitriana, Y. (2016). Journal of Tropical Agrotechnology Journal of Tropical Agrotechnology. Journal of Tropical Agrotechnology, 12(2), 259–269. [In Indonesian language]

Horticulture, D. J. (2018). Tips for Cultivating Oyster Mushrooms. Bogor. Center for Library and Dissemination of Agricultural Technology 39-65 [In Indonesian language]

Inayah, T., & Prima, E. (2022). Oyster Mushroom Cultivation and Processing as an Effort to Improve the Creative Economy of Beji Village. Agricultural Friday: Journal of Community Service, 3(2), 96–99. https://doi.org/10.32764/abdimasper.v3i2.2881 [In Indonesian language]

Jumriani, S., Wahyuni, S., Afifah Rahmadinah, N., Manguntungi, B., & Puspita Sari, A. (2023). Optimization of Oyster Mushroom Mycelium Growth (Pleurotus astreatus) with the Addition of Rice Straw Nutrition Integrated with the Inoculation Model. Journal of Biology Education Undiksha, 10(3), 14–24. [In Indonesian language]

Kurniati, F., Sunarya, Y., & Nurajijah, R. (2020). Growth and Yield of White Oyster Mushroom (Pleurotus Ostreatus (Jacq) P. Kumm) in Various Planting Media Compositions. Agricultural Media, 4(2), 59–68. https://doi.org/10.37058/mp.v4i2.1358 [In Indonesian language]

Kurniawan, F. (2022). The use of various types of agricultural waste as a medium for growing mushrooms. Bioedunis Journal, 1(2), 51–58. https://doi.org/10.24952/bioedunis.v1i2.6634 [In Indonesian language]

Nugroho, S. P. W., Baskara, M., & Moenandir, J. (2019). The Effects of Three Kind dnd Three Composition Planting Media Nutrient on White Oyster Mushroom. Journal of Plant Production, 7(9), 1725–1731. [In Indonesian language]

Nuradellia, M., Putri, F. D., Saputra, T. S., Amrullah, M. I., Syahriar, F. F., & Amin, R. (2023). Morphological characteristics and extent of oyster mushroom pileus due to differences in baglog media placement. Proceedings of the 2023 National Biology Seminar XI FMIPA Semarang, 183–189. [In Indonesian language]

Nurkameria, Imam Mahadi, Evi Suryawati. (2016). Effect of Npk Complex Fertilizers to the Growth and Protein Content of White Oyster Mushrooms (Pleurotus Ostreatus) as Module Development of Biology Learning of Concept Mushrooms ( Fungi ) in Class X Senior High School on Growth and Protein Levels. Agricultural Dynamics, 32(1), 1–15.

Pradana, H., Subardja, V. O., & Pirngadi, K. (2024). Effect of Organic Fertilizers, Merang Mushroom Waste and Biofertilizers on the Growth and Yield of Soybean Plants (Glycine max L. Merril) Grobogan Variety. Journal of Agroplasm, 11(2), 276–288. https://doi.org/10.36987/agroplasma.v11i2.5898 [In Indonesian language]

PT. Universal Eco Pacific. (2025). B3 Waste Management in the Wood Industry. Universaleco.Id.https://r.search.yahoo.com/_yltFuniversaleco.id%2Fpengelolaan-limbah-b3-industri kayu%2F/RK=2/RS=22A7svgarBlzGjTqFDVqg.xHL8w-

Puspita, D., & Widaryanto, E. (2020). The Effect of Oyster Mushroom Planting Media Waste as Planting Media and NPK Fertilizer on Potted Sunflowers ( Helianthus annuus L .). Journal of Plant Production, 8(9), 816–822. [In Indonesian language]

Ramdhoani, Luh Putu Yuni Widyastuti, & Ni Putu Eka Pratiwi. (2025). Growth and Production Yield of White Oyster Mushroom (Pleorotus ostreatus) in Organic Waste-Enriched Growth Media Variations. Indonesian Journal of Horticulture, 16(1), 24–31. https://doi.org/10.29244/jhi.16.1.24-31 [In Indonesian language]

Rosnina. (2017). The Effect of Adding Rice Husks to Various Planting Media on the Growth and Yield of White Oyster Mushroom (Pleurotus ostreotus). Journal of Agrium, 14(2), 18–25. [In Indonesian language]

Sholihah, N. H., Satyana, A., Department, K., Agriculture, B., Agriculture, F., Brawijaya, U., Veteran, J., & Timur, J. (2025). Effect of Composition of Oyster Mushroom Baglog Waste and NPK Fertilizer on the Growth and Quality of Marigold Plants (Tagetes erecta L.). Journal of Crop Production, 13(3), 191–201. http://dx.doi.org/10.21776/ub.protan.2025.013.03.06 [In Indonesian language]

Siska Apriyani, Budiyanto, H. B. (2015). Production and characteristics of white oyster fungus (Pleurotus ostreatus) in oil palm empty bunches (TKKS) media. 32, 1–9. [In Indonesian language]

Suparti, Lismiyati Marfuah. 2015. Productivity of White Oyster Mushrooms (Pleurotus Ostreatus) on Rice Husk Waste and Dried Banana Leaves as Alternative Media. Bioexperiment Journal 1 (2), 37-44. [In Indonesian language]

Suryani, T., & Carolina, H. (2017). Growth and yield of white oyster mushrooms in some nursery media materials. Bioexperiments: Journal of Biological Research, 3(1), 73. https://doi.org/10.23917/bioeksperimen.v3i1.3674 [In Indonesian language]

Susilo, H., Rikardo, R., & Suyamto, S. (2017). Utilization of sawdust waste as a cultivation medium for oyster mushrooms (Pleourotus ostreatus L.). Journal of Community Service, 2(1), 51–56. https://doi.org/10.30653/002.201721.16 [In Indonesian language]

Wahyuddin, Syam, Muh. Rizaldi Syam, 2021. Study of the Utilization of Sawdust as a Cultivation Medium for White Oyster Mushrooms. Lepa-lepa Journal. Volume 1 Number 3, 4-7. Open https: //ojs.unm.ac.id/JLLO/index [In Indonesian language]

Widyaningsih, E. (2023). Effect of NPK Fertilizer on the Growth and Yield of Various Rice Varieties (Oryza Sativa L.) with the Salibu System. Journal of Agros Agriculture, 25(3), 2823–2831. [In Indonesian language]




DOI: https://doi.org/10.36987/jpbn.v11i3.8222

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