Invasion of Endophytic Bacteria by Using Microencapsulation Technology as Stimulant in Cocoa Plants (Theobroma cacao L)

Gusti Yanda Prayoga, Kabul Warsito, Hanifah Mutia Z.N. Amrul

Abstract


Fertilization is an important process in the growth of cocoa (Theobroma cacao L). Fertilizing cocoa usually used inorganic fertilizers. Using of inorganic fertilizers continuously could degrading soil fertility. One of the efforts to replace inorganic fertilizers was using endophytic bacteria as biofertilizers. Some endophytic bacteria are abundant in healthy plant tissues. The aim of this research was to obtain endophytic auxin-producing bacteria in cocoa plants and determine the effect of seed immersion and microcapsules addition of endophytic bacteria on cocoa plant growth. The research design used was Complete Randomized Design (CRD), 16 treatments and 3 replications. The first factor was immersion of cocoa seed using endophytic bacteria suspension consisting of S0= 0 hours; S1= 5 hours; S2= 6 hours and S3= 7 hours and the second factor was microcapsules addition consisting of B0= 0 gr; B1= 5 gr; B2= 10 gr; B3= 15 gr. Isolation from the roots and stems of cocoa obtained 5 isolates endophyte bacteria. Auxin assays showed that five isolates were able to produce auxin. Observations on plant height showed the best treatment was treatment B1 (32.49 cm). Observation of total leaves showed S3 treatment was the highest data (11.83 strands). Observation rod diameter parameter, highest data was S3 treatment (4.01 mm). For leaf area parameter, highest value was B1 treatment (66.64 cm2). For wet weight parameter, highest data was S3 treatment (18.41 g). Root length parameter, highest data was B3 treatment (15.78 cm).  Test results showed that the application of suspension and microcapsules of endophytic bacteria significantly increased growth of cocoa.


Keywords


Auxin, Cocoa, Endophytic Bacteria, Microcapsules

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References


Ang, L. F., Darwis, Y., Por, L. Y., & Yam, M. F. (2019). Microencapsulation curcuminoids for effective delivery in pharmaceutical application. Pharmaceutics, 11(9). https://doi.org/10.3390/pharmaceutics11090451

Anjarwati, H., Waluyo, S., & Purwanti, S. (2017). Pengaruh Macam Media dan Takaran Pupuk Kandang Kambing terhadap Pertumbuhan dan Hasil Sawi Hijau (Brassica rapa L.). Vegetalika, 6(1), 35. https://doi.org/10.22146/veg.25983

Ariana, R. (2016). Potensi Bakteri Endofit dalam Meningkatkan Pertumbuhan Tanaman Tembakau yang Terinfeksi Nematoda Puru Akar (Meloidogyne spp.). 4(1), 1–23.

Aulia Meyuliana, Muharama Yora, Nur Salamah Harahap, Yuli Wahyu Eka Putri, Sri Rahmadina, Yelmi Wahyuni, Zulfi Marisa Elfian, Amalia Putri, & Ica Safitri. (2022). PENGARUH PEMBERIAN BEBERAPA DOSIS PUPUK ORGANIK CAIR (POC) TERHADAP PERTUMBUHAN BIBIT KAKAO (Theobroma cacao L.). Jurnal Riset Perkebunan, 3(1), 12–17. https://doi.org/10.25077/jrp.3.1.12-17.2022

Fendy, E., & Paramita, V. (2021). Optimasi Proses Mikroenkapsulasi Minyak Kelapa Murni d engan Bahan Penyalut β -Cyclodextrin. 17(1), 27–34.

Giyanto, G., & Anwar Arifin, F. (2019). FORMULASI BAKTERI ENDOFIT ASAL TANAMAN KAKAO (Theobroma cacao L.) SEBAGAI BIOPESTISIDA FAUZI ANWAR ARIFIN. October. https://www.researchgate.net/publication/336588446

Herlina, L., Pukan, K. K., & Mustikaningtyas, D. (2016). Kajian Bakteri Endofit Penghasil IAA ( Indole Acetic Acid ) untuk Pertumbuhan Tanaman. J. FMIPA, Universitas Negeri Semarang, 14(1), 51–58.

HERLINA, L., PUKAN, K. K., & MUSTIKANINGTYAS, D. (2017). The endophytic bacteria producing IAA (Indole Acetic Acid) in Arachis hypogaea. Cell Biology and Development, 1(1), 31–35. https://doi.org/10.13057/cellbioldev/v010106

Jamidi, J., Faisal, F., & Fadhil Ichsan, M. (2021). Aplikasi Pupuk Organik Cair Limbah Kulit Nanas Dan Pukan Sapi Terhadap Pertumbuhan Bibit Kakao (Theobroma cacao, L.). Jurnal Agrium, 18(2), 145–153. https://doi.org/10.29103/agrium.v18i2.5332

Jia, X., Liu, P., & Lynch, J. P. (2018). Greater lateral root branching density in maize improves phosphorus acquisition from low phosphorus soil. Journal of Experimental Botany, 69(20), 4961–14790. https://doi.org/10.1093/jxb/ery252

Khaeruni, A., Nirmala, T., Siti Anima Hisein, W., Gusnawaty, G., Wijayanto, T., & Kade Sutariati, G. A. (2020). Potensi dan Karakterisasi Fisiologis Bakteri Endofit Asal Tanaman Kakao Sehat sebagai Pemacu Pertumbuhan Benih Kakao. Jurnal Ilmu Pertanian Indonesia, 25(3), 388–395. https://doi.org/10.18343/jipi.25.3.388

Maghfoer, M. D. (2018). Teknik Pemupukan Terung Ramah Lingkungan.

Marwan, H., Nusifera, S., & Mulyati, S. (2021). Potensi Bakteri Endofit sebagai Agens Hayati untuk Mengendalikan Penyakit Blas pada Tanaman Padi. Jurnal Ilmu Pertanian Indonesia, 26(3), 328–333. https://doi.org/10.18343/jipi.26.3.328

Moshinsky, M. (2020). Potensi kultur campuran bakteri endofit sebagai pemacu pertumbuhan bibit tanaman karet. Nucl. Phys., 13(1), 104–116.

Nugroho, H. C., Moeljanto, B. D., Supandji, S., & Probojati, R. T. (2021). Optimasi Komposisi Media Tanam dan Dosis Pupuk Organik Cair (POC) Terhadap Pertumbuhan Awal Bibit Kakao (Theobroma cacao L.). JINTAN : Jurnal Ilmiah Pertanian Nasional, 1(2), 180. https://doi.org/10.30737/jintan.v1i2.1827

Panichikkal, J., Prathap, G., Nair, R. A., & Krishnankutty, R. E. (2021). Evaluation of plant probiotic performance of Pseudomonas sp. encapsulated in alginate supplemented with salicylic acid and zinc oxide nanoparticles. International Journal of Biological Macromolecules, 166, 138–143. https://doi.org/10.1016/j.ijbiomac.2020.10.110

Permadi, B. (2021). Pengaruh Pupuk Organik Cair (POC) Urine Kambing Terhadap Pertumbuhan Bibit Kakao (Theobroma cacao L.). Jurnal Mahasiswa Agroteknologi (Jmatek) Bayu Permadi, 2(1), 35–40. https://jurnal.ulb.ac.id/index.php/JMATEK/article/view/2030

Pertanian, K., Gedung, C., & No, J. H. R. M. (2015). DEWAN KAKAO INDONESIA ( DEKAINDO ) LAPORAN PENYELENGGARAAN LOKAKARYA “ MENYONGSONG PEMBERLAKUAN PERATURAN MENTERI PERTANIAN †TENTANG PERSYARATAN MUTU DAN PEMASARAN BIJI KAKAO. 3, 5–8.

Pinem, Agroekoteknologi, J., Usu, F. P., & No, E. (2019). Pengujian Bakteri Endofit Asal Cabai Dalam Menekan Pertumbuhan F. Oxysporum F.Sp. Capsici Penyebab Penyakit Layu Fusarium Pada Cabai. Agroekoteknologi, 7(2), 339–346.

Pradana, A. P., Munif, A., & S, S. (2020). Formulasi Konsorsium Bakteri Endofit untuk Menekan Infeksi Nematoda Puru Akar Meloidogyne incognita pada Tomat. Techno: Jurnal Penelitian, 9(2), 390. https://doi.org/10.33387/tjp.v9i2.2210

Prihatiningsih, N., Djatmiko, H. A., & Lestari, P. (2021). Endophytic bacteria associated with rice roots from suboptimal land as plant growth promoters. Biodiversitas, 22(1), 432–437. https://doi.org/10.13057/biodiv/d220153

Puspita, F., Saputra, S. I., & Merini, D. J. (2019). Uji Beberapa Konsentrasi Bakteri Bacillus sp. Endofit untuk Meningkatkan Pertumbuhan Bibit Kakao (Theobroma cacao L.). Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 46(3), 322–327. https://doi.org/10.24831/jai.v46i3.16342

Putri, D., Munif, A., & Mutaqin, K. H. (2016). Lama Penyimpanan, Karakterisasi Fisiologi, dan Viabilitas Bakteri Endofit Bacillus sp. dalam Formula Tepung. Jurnal Fitopatologi Indonesia, 12(1), 19–26. https://doi.org/10.14692/jfi.12.1.19

RiauWati, R., & Chaerunisaa, A. Y. (2020). REVIEW TEKNIK MIKROENKAPSULASI PADA EKSTRAK MANGOSTEEN (A Review Of Microencapsulation Techniques In Mangosteen Extract). Journal of Current Pharmaceutical Science, 3(2), 2598–2095.

Rosyida, R., & Nugroho, A. S. (2017). PENGARUH DOSIS PUPUK NPK MAJEMUK DAN PGPR (PLANT GROWTH PROMOTING RHIZOBACTERIA) TERHADAP BOBOT BASAH DAN KADAR KLOROFIL DAUN TANAMAN PAKCOY (Brassica rapa L.). Bioma : Jurnal Ilmiah Biologi, 6(2), 42–56. https://doi.org/10.26877/bioma.v6i2.1716

Sabahannur, S., Nirwana, N., & Subaedah, S. (2016). Kajian Mutu Biji Kakao Petani Di Kabupaten Luwu Timur, Soppeng Dan Bulukumba. Jurnal Industri Hasil Perkebunan, 11(2), 59. https://doi.org/10.33104/jihp.v11i2.3412

Salo, E. N., & Novero, A. (2020). Identification and characterisation of endophytic bacteria from coconut (Cocos nucifera) tissue culture. Tropical Life Sciences Research, 31(1), 57–68. https://doi.org/10.21315/tlsr2020.31.1.4

Saputri, Y., Advinda, L., Chatri, M., & Handayani, D. (2020). Potential Bacillus sp. in Producing Indole Acetic Acid (IAA) and Its Effect on Sprouts Root Length of Red Chili Seeds (Capsicum annuum L.) Potensi Bacillus sp. dalam Menghasilkan Indole Acetic Acid (IAA) serta Pengaruhnya terhadap Panjang Akar Kecambah Be. 5(2), 96–105.

Saridewi, L. P., Prihatiningsih, N., & Djatmiko, H. A. (2020). Karakterisasi biokimia bakteri endofit akar terung sebagai pemacu pertumbuhan tanaman dan pengendali penyakit layu bakteri in planta. Jurnal Proteksi Tanaman Tropis, 1(1), 1. https://doi.org/10.19184/jptt.v1i1.15579

Singh, R., Pandey, K. D., Singh, M., Singh, S. K., Hashem, A., Alâ€arjani, A. B. F., Abd_allah, E. F., Singh, P. K., & Kumar, A. (2022). Isolation and Characterization of Endophytes Bacterial Strains of Momordica charantia L. and Their Possible Approach in Stress Management. Microorganisms, 10(2), 0–13. https://doi.org/10.3390/microorganisms10020290

Sriharti, S., & Dyah, S. (2018). Utilization of cacao waste (Theobroma cacao L) for composting by using various activator materials. AIP Conference Proceedings, 2024(2018). https://doi.org/10.1063/1.5064337

Suryani, N., Suzanti Betha, O., Fakultas, M., Kesehatan, I., Syarif, U., & Jakarta, H. (2019). UJI VIABILITAS MIKROENKAPSULASI Lactobacillus casei MENGGUNAKAN MATRIK NATRIUM ALGINAT Microenkapsulation Viability Test Of Lactobacillus Casei Using Sodium Alginate Matric. Jurnal Farmasi Lampung, 8(1), 1–8. http://digilib.utb.ac.id/index.php/jfl/article/download/80/75

Usrin, U., Pamungkas, D. H., & Widata, S. (2019). RESPON PERTUMBUHAN BIBIT KAKAO ( Theobroma cacao L.) TERHADAP PEMBERIAN PUPUK KANDANG KAMBING DAN PUPUK ORGANIK CAIR (POC). RESPON PERTUMBUHAN BIBIT KAKAO ( Theobroma Cacao L.) TERHADAP PEMBERIAN PUPUK KANDANG KAMBING DAN PUPUK ORGANIK CAIR (POC), July, 65–83.

Walida, H., Harahap, F. S., Hasibuan, M., & Yanti, F. F. (2019). Isolasi Dan Identifikasi Bakteri Penghasil Iaa Dan Pelarut Fosfat Dari Rhizosfer Tanaman Kelapa Sawit. BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan), 6(1), 1. https://doi.org/10.31289/biolink.v6i1.2090

Wati, L., Nurhayati, N., & Hasanuddin, H. (2021). Pengaruh Media Tanam dan Konsentrasi Pupuk Organik Cair terhadap Pertumbuhan Bibit Kakao (Theobroma cacao L.). Jurnal Ilmiah Mahasiswa Pertanian, 6(4), 801–808. https://doi.org/10.17969/jimfp.v6i4.18254

Yusuf, H., Sahputra, R., & Sah, R. I. (2018). Pengaruh media tanam dan pemberian pupuk organik cair terhadap pertumbuhan bibit kakao (Theobroma cacao, L). Jurnal Penelitian …, 5(1), 1–11. http://jurnal.unsam.ac.id/index.php/jagrs/article/view/850

Zabot, G. L., Schaefer Rodrigues, F., Polano Ody, L., Vinícius Tres, M., Herrera, E., Palacin, H., Córdova-Ramos, J. S., Best, I., & Olivera-Montenegro, L. (2022). Encapsulation of Bioactive Compounds for Food and Agricultural Applications. Polymers, 14(19). https://doi.org/10.3390/polym14194194

Zain, N. M., Bachtiar, T., & Sugoro, I. (2018). Kontribusi Nitrogen dari Bakteri Endofit pada Tanaman Padi. Jurnal Ilmiah Aplikasi Isotop Dan Radiasi, 14(1), 1. https://doi.org/10.17146/jair.2018.14.1.4152




DOI: https://doi.org/10.36987/jpbn.v9i2.3908

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