Duration Effects of Shrimp Paste Storage on ALT of Mold Colonies: Variations by Brand and Pre-Standard Treatment

Nur Laila Syafitri, Utami Sri Hastuti, Sitoresmi Prabaningtyas

Abstract


Shrimp paste is a commonly employed condiment or flavoring in culinary preparations. As a result of the nutrients present in shrimp paste, fungi and other microbial contaminants are able to proliferate and develop on the paste. This study aims as follows: 1) to determine ALT measurements of mold colonies to assess the quality of two brands of pre-treated shrimp paste; and 2) to identify contaminating mold species present in steamed and unsteamed shrimp paste prior to storage. Descriptive quantitative and qualitative research methods were employed within the Biology Department of FMIPA UM's Microbiology Laboratory. A series of three samplings were conducted on treated shrimp paste brands A and B, one week apart between each sampling. A volume of 90 ml of 0.1% peptone water was used to dissolve 10 grams of shrimp paste, resulting in a dilution of 10-1; this process was repeated until the dilution reached 10-5. 0. The surface of PDA (Potato Dextrose Agar) medium was inoculated with 1 ml of suspension from each dilution. The medium was subsequently incubated at a temperature of 25oC for 7x24 hours. The findings of this study indicate the following: 1) At the 28th day of storage, both brands of shrimp paste that underwent treatment with steamed and unsteamed shrimp paste remained of acceptable quality for human consumption. 2) In steamed and unsteamed shrimp paste, thirteen species of mold contaminants were identified: Chrysosporium corda, Cladosporium sphaerospermum, Penicillium frequentans, Penicillium chrysogenum, Rhizoctonia sp1., Aspergillus candidus, Fusarium equeseti, Colletotrichum ti, Moniliella acetobutens, and Rhizoctonia sp3

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References


Anto Aris, J., Nur, R. M., & Saibi, N. (2021). Isolasi kapang kontaminan pada produk komersial ikan asin di morotai selatan, pulau morotai. Aurelia Journal, 3(1), 89–95.

Aristyan, I., Ibrahim, R., & Rianingsih, L. (2014). Pengaruh perbedaan kadar garam terhadap mutu organoleptik dan mikrobiologis terasi rebon (Acetes sp.). Jurnal Pengolahan Dan Bioteknologi Hasil Perikanan, 3(2), 60–66.

Artanti, D., Adilah, F. U., & Azizah, F. (2019). Pemeriksaan jumlah kapang sebagai indikator kualitas terasi di pasar tambaksari surabaya. The Journal of Muhammadiyah Medical Laboratory, 2(2), 56.

Danarsi, C. S., & Noer, E. R. (2016). Pengaruh lama penyimpanan terhadap mutu mikrobiologi makanan pendamping air susu ibu (mpasi) bubur instan dengan subtitusi tepung ikan gabus dan tepung labu kuning. Journal of Nutrition College, 5(2), 58–63.

Fardiaz, S. (1992). mikrobiologi pangan 1. Gramedia Pustaka Utama.

Fitria, Suhartini, & Prihandono, D. S. (2023). Gambaran Kapang Aspergillus Sp pada Terasi dalam Kemasan Tanpa Merek di Pasar Tradisional Kota Samarinda. JI-KES (Jurnal Ilmu Kesehatan), 6(2), 147–154. https://doi.org/10.33006/jikes.v6i2.575

Gandjar, I., Sjamsuridzal, W., & Oetari, A. (2006). Mikologi Dasar dan Terapan. Yayasan Obor Indonesia.

Hafsan. (2011). Mikrobiologi Umum. Vol. 1999. Issue December. Alauddin University Press.

Hastuti, utami sri. (2021). penuntun praktikum mikologi. umm press.

Hastuti, U. S., Dipu, Y. V., & Mariyanti. (2011). Isolasi dan identifikasi mikroflora kapang kontaminan pada kue pia yang dijual di kota malang. Seminar Nasional VIII Pendidikan Biologi 15, 51–62.

Nasution, M. Y., Hasairin, A., & Harsono, T. (2011). Kajian Keragaman Jenis dan Laju Pertumbuhan Kapang dalam Acar Limau Kasturi (Citrofortunella microcarpa) Makanan Masyarakat Melayu. In Jurnal Penelitian Saintika (Vol. 11, Issue 2, pp. 115–119).

Nur Cahyo, M. F., Hastuti, S., & Maflahah, I. (2016). Penentuan umur simpan terasi instan dalam kemasan. Agrointek, 10(1), 55–61.

Nurholipah, N., & Ayun, Q. (2021). ISOLASI DAN IDENTIFIKASI Rhizopus oligosporus DAN Rhizopus oryzae PADA TEMPE ASAL BEKASI. Jurnal Teknologi Pangan, 15(1), 98–104. https://doi.org/10.33005/jtp.v15i1.2742

Putri, R. M. S., Nurjanah., & Tarman, K. (2018). Analisis Kuantitatif Mikrobiologi Serbuk Minuman Fungsional Lintah Laut (Discodoris sp.) pada Suhu yang Berbeda Selama Penyimpanan. Jurnal Ilmiah Biologi Biosfera: A Scientific Journal, 35(3), 124–130. https://doi.org/10.20884/1.mib.2018.35.3.729

Sahay, S. (2022). Extremophilic Fungi. In Extremophilic Fungi. https://doi.org/10.1007/978-981-16-4907-3

Sakti, H., Lestari, S., & Supriadi, A. (2016). Perubahan Mutu Ikan Gabus (Channa striata) Asap Selama Penyimpanan. Jurnal Teknologi Hasil Perikanan, 5(1), 11–18.

Suprapti, L. (2002). Teknologi tepat guna cara membuat terasi. Kanisius.

Suryani, Y., Taupiqurrahman, O., & Kulsum, Y. (2020). Mikologi. PT Freeline Cipta Granesia.

Thapa, N., & Pal, J. (2002). Studies on microbial diversity associated with some fish products of the eastern himalayas. Zoology, 156.

Wiryosoendjojo, K., Puspawati, N., & Sulistyawati, D. (2019). Isolasi dan Identifikasi Jamur Xerofilik pada Kopi Instant. Biomedika, 12(1), 112–120. https://doi.org/10.31001/biomedika.v12i1.411




DOI: https://doi.org/10.36987/jpbn.v9i3.4690

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