Screening For Lipolytic Bacteria from Bonoloyo Cemetery, Surakarta

Heni Sulistyo Rini, Erma Musbita Tyastuti, Yasir Siddiq, Triastuti Rahayu

Abstract


A cemetery is a burial place managed by the government. Inside the TPU, the body is decomposed by microorganisms, one of which is lipolytic bacteria, because the human body is composed of about 12.5–13.60% lipids. Research on lipolytic bacteria from TPU in Indonesia has not been found, even though it has the potential to obtain lipolytic bodies. Therefore, this research was conducted to select lipolytic bacteria from TPU and provide simple assistance. A total of 45 bacterial isolates from TPU Bonoloyo Surakarta were selected for their lipolytic activity using tributyrin agar media. Lipolytic activity was determined using the lipolytic index (LI). Bacterial isolates with lipolytic potential were identified based on colony morphology and Gram staining. The results showed that 30 isolates (67%) showed lipolytic activity, with the highest LI value of 5.43 (BLB 9) after 2x24 hours of incubation. Bacterial isolates that have lipolytic activity are white colonies with circular shapes. The results of Gram staining showed that the bacterial isolates belonged to a group of Gram-negative bacteria in the form of cocci. The conclusion put forward is that public cemeteries (TPU) have the potential to store isolates of lipolytic bacteria dominated by Gram-positive bacteria by as much as 57%


Keywords


Bonoloyo, Cemetery, Screening, Lipolytic Bacteria, Tributirin

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References


Amalia, G. (2011). Ketersediaan Lahan Tempat Pemakaman Umum (TPU) di Kota Surakarta. 8.

Brilliana Putra, A., Fakhrurizal Amin, I., Dini Fitriyasa, R., & Safitry, O. (2020). Memperkirakan saat Kematian (Postmortem Interval) Menggunakan Temuan Mikrobiom pada Setiap Tahap-Tahap Penguraian (Decomposition): An Evidence-Based Case Report. Journal of Indonesian Forensic and Legal Medicine, 2(1), 111–122.

Carrazco-Palafox, J., Rivera-Chavira, B. E., Ramírez-Baca, N., Manzanares-Papayanopoulos, L. I., & Nevárez-Moorillón, G. V. (2018). Improved method for qualitative screening of lipolytic bacterial strains. MethodsX, 5, 68–74. https://doi.org/10.1016/j.mex.2018.01.004

Chairunnisa, C., Riyanto, R., & Karim, A. (2019). Isolasi dan Uji Bakteri Lipolitik dalam Mendegradasi Minyak Pada Limbah Cair Kelapa Sawit di Kebun Marihat, Pematang Siantar. Jurnal Ilmiah Biologi UMA (JIBIOMA), 1(2), 44–52. https://doi.org/10.31289/jibioma.v1i2.155

Dahliaty, A., Susanti, R., & Haryani, Y. (2012). Skrining Bakteri Lipolitik dari Sungai Siak di Daerah Pelita Pantai Kota Pekanbaru. J. Ind. Che. Acta, 3(1), 1–4.

Dent, B. B., Forbes, S. L., & Stuart, B. H. (2004). Review of human decomposition processes in soil. Environmental Geology, 45(4), 576–585. https://doi.org/10.1007/s00254-003-0913-z

Elyza, F., Gofar, N., & Munawar, M. (2016). Identifikasi Dan Uji Potensi Bakteri Lipolitik Dari Limbah Sbe (Spent Bleaching Earth) Sebagai Agen Bioremediasi. Jurnal Ilmu Lingkungan, 13(1), 12. https://doi.org/10.14710/jil.13.1.12-18

Fardiaz, S. (1992). Mikrobiologi Pangan. Gramedia Pustaka Utama.

FORBES, R. M., COOPER, A. R., & MITCHELL, H. H. (1953). The composition of the adult human body as determined by chemical analysis. The Journal of Biological Chemistry, 203(1), 359–366. https://doi.org/10.1016/s0021-9258(19)52646-1

Houde, A., Kademi, A., & Leblanc, D. (2004). Lipases and their industrial applications: An overview. Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, 118(1–3), 155–170. https://doi.org/10.1385/ABAB:118:1-3:155

Jeager, B. (2015). Uji Kualitatif dan Kuantitatif Isolat Bakteri Lipolitik dari Limbah Cair Pabrik Pengolahan Ikan Kecamatan Muncar, Banyuwangi. Jurnal Biotropika, 3(3), 2–6.

Khairani, & Manalu, K. (2023). ISOLASI DAN IDENTIFIKASI BAKTERI LIPOLITIK DARI LIMBAH CAIR KELAPA SAWIT (Elaeis quineensis Jacq.). BIOEDUSAINS: Jurnal Pendidikan Biologi Dan Sains, 6(1), 1–13.

Leoni F. (2019). Isolasi dan Identifikasi Bakteri Lipolitik Pada Proses Pengomposan Limbah Domestik. F. Leoni, 53(9), 1689–1699.

Melati, I. (2020). Pusat Penelitian Limnologi LIPI. Rahayu 2005, 272–286.

Oktavia, D. A., & Wibowo, S. (2017). Penapisan dan Identifikasi Bakteri Lipolitik yang Diisolasi dari Air Limbah Pengolahan Surimi dan Pengalengan Rajungan. Jurnal Pascapanen Dan Bioteknologi Kelautan Dan Perikanan, 11(2), 147. https://doi.org/10.15578/jpbkp.v11i2.262

Rahmawati, L. R., Adlina, S., & Yuliana, A. (2021). Isolasi dan Identifikasi Bakteri Penghasil Protease Ekstraseluler dari Limbah Cair Tahu Putih. Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu Keperawatan, Analis Kesehatan Dan Farmasi, 21(2), 187–193.

Ramnath, L., Sithole, B., & Govinden, R. (2017). Identification of lipolytic enzymes isolated from bacteria indigenous to Eucalyptus wood species for application in the pulping industry. Biotechnology Reports, 15(July), 114–124. https://doi.org/10.1016/j.btre.2017.07.004

Rosdi, A., Dahalan, F. A., Zhan, L. Z., Babakhani, P., Shams, S., Engineering, C., & Area, P. (2022). Collection of samples. 2, 1–5.

Saputri, R. D., Tyastuti, E. M., Sidiq, Y. & Rahayu, T. (2023). Public Cemetery’s Potency as The Source of Proteolytic Bacteria. Quagga: Jurnal Pendidikan dan Biologi, 15(2), 201-206. doi: 10.25134/quagga.v15i2.44Shreshta R, Kanchan T, K. K. (2020). Methods of estimation of time since death. In StatPearls Publishing. StatPearls Publishing.

Singh B, Minick KJ, Strickland MS, et al. (2018). Temporal and Spatial Impact of Human Cadaver Decomposition on Soil Bacterial and Arthropod Community Structure and Function. Front Microbiol, 8(1), 2616.

Student, M. T., Kumar, R. R., Omments, R. E. C., Prajapati, A., Blockchain, T.-A., Ml, A. I., Randive, P. S. N., Chaudhari, S., Barde, S., Devices, E., Mittal, S., Schmidt, M. W. M., Id, S. N. A., PREISER, W. F. E., OSTROFF, E., Choudhary, R., Bit-cell, M., In, S. S., Fullfillment, P., … Fellowship, W. (2021). No 主観的å¥åº·æ„Ÿã‚’中心ã¨ã—ãŸåœ¨å®…高齢者ã«ãŠã‘ã‚‹ å¥åº·é–¢é€£æŒ‡æ¨™ã«é–¢ã™ã‚‹å…±åˆ†æ•£æ§‹é€ 分æžTitle. Frontiers in Neuroscience, 14(1), 1–13.

Suyanto, E., Soetarto, E. S., & Cahyanto, M. N. (2015). Produk Lipase Kapang Lipolitik pada Limbah Ampas Kelapa. Bioeksperimen: Jurnal Penelitian Biologi, 1(1), 12–17. https://doi.org/10.23917/bioeksperimen.v1i1.311

Syarifah, S.N., Rahayu, T., Tyastuti, E.M., & Sidiq, Y. 2023. Screening andIdentification of CellulolyticBacteria from PublicCemetery Soil.BIOEDUSCIENCE, 7(2), 192-199.

Szymczak, T., Cybulska, J., Podleśny, M., & Frąc, M. (2021). Various perspectives on microbial lipase production using agri-food waste and renewable products. Agriculture (Switzerland), 11(6). https://doi.org/10.3390/agriculture11060540

Tang, L., Chen, M. H., Nie, X. C., Ma, M. R., & Qiu, L. H. (2017). Dyella lipolytica sp. nov., a lipolytic bacterium isolated from lower subtropical forest soil. International Journal of Systematic and Evolutionary Microbiology, 67(5), 1235–1240. https://doi.org/10.1099/ijsem.0.002020

Tsani, Fauqi. Falakhus. 2021. (Isolation and Characteristic of Lipolytic Bacteria from the Soil of the Final Processing Site (TPA) Talangagung Kepanjen, Malang). Maulana Malik Ibrahim Islamic University of Malang.




DOI: https://doi.org/10.36987/jpbn.v9i3.4944

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