Antibiotic-Resistant Lactic Acid Bacteria Pediococcus acidilactici Isolated from Buffalo’s Milk Fermentation (Dadiah) Bukittinggi, West Sumatera

Nadia Purnama Dewi, Dessy Abdullah, Yudha Endra Pratama

Abstract


The use of antibiotics is still carried out in the health world so that it will cause a big problem, namely antibiotic resistance. The use of antibiotics has also been limited but cannot be stopped. The use of probiotics as therapy in the world of health is increasing every year, so it is necessary to study the combination of antibiotics and probiotics so as not to reduce the effectiveness of probiotics later. This study aims to determine the resistance ability of lactic acid bacteria (LAB) isolates from Buffalos Milk Fermentation (called as dadiah) Bukittinggi against antibiotics. This research uses a descriptive, experimental method where the observations made are related to the total mass of isolates per ml and the clear zone of LAB isolates from dadiah Bukittinggi against antibiotics. In this study, five types of LAB isolates from dadiah Bukittinggi were used, and it was found that the total mass of isolates ranged from 4-194 x108 CFU/ml. In addition to antibiotic resistance, it was known that dadiah LAB isolates resisted Kanamycin and Penicillin by 75%. While on the antibiotic chloramphenicol, ampicillin and tetracyclin, the four isolates are suspect to the antibiotic. The five LAB isolates of dadiah used in the study, DS1 isolate had the best total mass and was resistant to kanamycin and penicillin


Keywords


Antibiotic; dadiah; Lactic acid bacteria; Probiotic; Pediococcus acidilactici

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References


Amelia, R., Abdullah, D., Pratama, Y. E., & Purwati, E. (2021). Antimicrobial Activity of Lactic Acid Bacteria Found in Dadiah on Disease-Causing Skin Infections. Indian Journal of Forensic Medicine & Toxicology, 15(2), 3314–3318. https://doi.org/10.37506/ijfmt.v15i2.14887

Ammor, M. S., Flórez, A. B., & Mayo, B. (2007). Antibiotic resistance in non-enterococcal lactic acid bacteria and bifidobacteria. Food Microbiology, 24(7), 559–570. https://doi.org/https://doi.org/10.1016/j.fm.2006.11.001

Binda, S., Hill, C., Johansen, E., Obis, D., Pot, B., Sanders, M. E., Tremblay, A., & Ouwehand, A. C. (2020). Criteria to Qualify Microorganisms as “Probiotic” in Foods and Dietary Supplements. Frontiers in Microbiology, 11, 1662. https://doi.org/https://doi.org/10.3389/fmicb.2020.01662

Cavalieri, S.J., Harbeck R.J., Rankin I. D., Sautter R.L., McCarter Y.S., Ortez J.H., Sharp S.E., & Spiegel C.A. (2005). Manual of Antimicrobial Susceptibility Testing. American Society for Microbiology. 241 p

D’aimmo, M. ., Modesto, M., & Biavati, B. (2007). Antibiotic resistance of lactic acid bacteria and Bifido-bacterium spp. isolated from dairy and pharmaceutical products. International Journal of Food Microbiology, 115, 35–42.

Daniali, M., Nikfar, S., & Abdollahi, M. (2020). Antibiotic resistance propagation through probiotics. Expert Opinion on Drug Metabolism & Toxicology, 12, 1207–1215. https://doi.org/https://doi.org/10.1080/17425255.2020.1825682

Dewi, N. P., Poddar, S., Rai, R. P., Purwati, E., Abdullah, D., & yudha. (2023). The Potential Lactic Acid Bacteria from Dadiah Sianok bukittinggi City, West Sumatera as Probiotic. Research Journal of Pharmacy and Technology, 16(2). https://doi.org/10.52711/0974-360X.2023.00107

Duche, R. T., Singh, A., Wandhare, A. G., Sangwan, V., Sihag, M. K., Nwagu, T. N. T., Panwar, H., & Ezeogu, L. (2023). Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria. BMC Microbiology, 23(1), 142. https://doi.org/https://doi.org/10.1186/s12866-023-02883-0

Eaton, T. J., & Gasson, M. J. (2001). Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Applied and Environmental Microbiology, 64(4), 1628–1635. https://doi.org/https://doi.org/10.1128/AEM.67.4.1628-1635.2001

EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP). (2012). Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance. EFSA Journal, 10(6), 2740. https://doi.org/10.2903/j.efsa.2012.2740

Erginkaya, Z., Turhan, E. U., & Tatli, D. (2018). Determination of antibiotic resistance of lactic acid bacteria isolated from traditional Turkish fermented dairy products. Iranian JOurnal of Veterinary Research, 19(1), 53–36.

FAO. (2001). Probiotics in Food: Health and Nutritional Properties and Guidelines for Evaluation. Joint FAO/WHO. https://www.fao.org/3/y6398e/y6398e.pdf

Flórez, A. ., Delgado, S., & Mayo, B. (2005). Antimicrobial susceptibility of lactic acid bacteria isolated from a cheese environment. Canadian Journal Microbiology, 51, 51–58.

Gevers, D., Danielsen, M., Huys, G., & Swings, J. (2003). Molecular characterization of tet(M) genes in Lactobacillus isolates from different types of fermented dry sausage. Applied and Environmental Microbiology, 69(2), 1270–1275. https://doi.org/. https://doi.org/10.1128/AEM.69.2.1270-1275.2003

Grossman, T. H. (2016). Tetracycline Antibiotics and Resistance. Cold Spring Harbor Perspectives in Medicine, 6(4). https://doi.org/https://doi.org/10.1101/cshperspect.a025387

Herreros, M. ., Sandoval, H., Gonzalez, L., Castroj, M., Frenso, J. ., & Tornadijo, M. . (2005). Antimicrobial activitiy and antibiotic resistance of lactic acid bacteria isolated from armada cheese (a spanish goats’ milk cheese) spain. Food Microbiology, 22, 455–459.

Land, M. H., Rouster-Stevens, K., Woods, C. R., Cannon, M. L., Cnota, J., & Shetty, A. K. (2005). Lactobacillus sepsis associated with probiotic therapy. Pediatrics, 115(1), 178–181. https://doi.org/https://doi.org/10.1542/peds.2004-213

Mathur, S., & Singh, R. (2005). Antibiotic resistance in food lactic acid bacteria — a review. 105, 281–295. https://doi.org/10.1016/j.ijfoodmicro.2005.03.008

Melia, S., Yuherman, Jaswandi, & Purwati, E. (2018). Selection of Buffalo Milk Lactic Acid Bacteria with Probiotic Potential. Asian Journal of Pharmaceuticl and Clinical Research, 11(6), 9–12.

Nasab, T. J., Khaleghi, M., Farsinejad, A., & Khorrami, S. (2021). Probiotic potential and anticancer properties of Pediococcus sp. isolated from traditional dairy products. Biotechnology Report, 29.

Panwar, H., Rokana, N., Aparna, S. ., Kaur, J., Singh, A., Singh, J., & Puniya, A. . (2021). Gastrointestinal stress as innate defence against microbial attack. Journal Applied Microbiology, 130(4), 61.

Purwati, E., Syukur, S., & Hidayat, Z. (2005). Lactobacilus sp. isolation of Biovicophitomegaas a probiotic. Prosiding Seminar Nasional Bioteknologi. Lembaga Ilmu Pengetahuan Indonesia.

Roos, S., Karner, F., Axelsson, L., & Jonsson, H. (2002). Lactobacillus mucosae sp. nov., a new species with in vitro mucus binding activity isolated from pig intestine. Int. J. Syst. Evol. Microbiol, 50, 251–258.

Salyers, A. A., Gupta, A., & Wang, Y. (2004). Human intestinal bacteria as reservoirs for antibiotic resistance genes. Trends in Miicrobiology, 12(9), 412–416. https://doi.org/https://doi.org/10.1016/j.tim.2004.07.004

Tang, K. W. K., Millar, B. C., & Moore, J. E. (2023). Antimicrobial Resistance (AMR). British Journal of Biomedical Science, 80, 11387. https://doi.org/https://doi.org/10.3389/bjbs.2023.11387

Temmerman, R., Pot, B., Huys, G., & Swings, J. (2003). Identification and antibiotic susceptibility of bacterial ısolates from probiotic products. International Jornal of Food Microbiology, 81, 1–10.

Thakur, N., Rokana, N., & Panwar, H. (2016). Probiotics, selection criteria, safety and role in health and disease. Journal Innovative Biology, 3(1), 259.

Wang, K., Zhang, H., Feng, J., Ma, L., Fuente-Núñez, C. D. L., Wang, S., & Lu, X. (2019). Antibiotic resistance of lactic acid bacteria isolated from dairy products in Tianjin, China. Journal of Agriculture and Food Research, 1, 1006. https://doi.org/https://doi.org/10.1016/j.jafr.2019.100006

WHO. (2002). Joint FAO/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. https://www.who.int/foodsafety/fs_management/en/probiotic_guidelines.pdf

Wong, A., Matijasic, B. B., Ibana, J. A., & Lim, R. L. H. (2022). Editorial: Antimicrobial Resistance Along the Food Chain: Are We What We Eat? Frontiers in Microbiology, 13, 881882. https://doi.org/https://doi.org/10.3389/fmicb.2022.881882




DOI: https://doi.org/10.36987/jpbn.v10i2.5699

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