Antibiotic-Resistant Lactic Acid Bacteria Pediococcus acidilactici isolated from Buffalo’s Milk Fermentation (Dadiah) Bukittinggi, West Sumatera

Nadia Purnama Dewi, Dessy Abdullah, Yudha Endra Pratama

Abstract


The use of antibiotics is still carried out in the health world so that it will cause a big problem, namely antibiotic resistance. The use of antibiotics has also been limited but cannot be stopped. The use of probiotics as therapy in the world of health is increasing every year, so it is necessary to study the combination of antibiotics and probiotics so as not to reduce the effectiveness of probiotics later. This study aims to determine the resistance ability of lactic acid bacteria (LAB) isolates from dadiah bukittinggi against antibiotics. This research uses a descriptive, experimental method where the observations made are related to the total mass of isolates per ml and the clear zone of LAB isolates from dadiah bukittinggi against antibiotics. In this study using five types of LAB isolates from dadiah Bukit Tinggi, it was found that the total mass of isolates ranged from 4-194 x108 CFU/ml. As well as antibiotic resistance, it was known that dadiah LAB isolates had resistance to the antibiotic Kanamycin and Penicillin 75%. While on the antibiotic chloramphenicol, ampicillin and tetracyclin, the four isolates are suspect to the antibiotic. Of the five dadiah LAB isolates used in the study, DS1 isolate had the best total mass and was resistant to kanamycin and penicillin

Keywords


Antibiotic; dadiah; Lactic acid bacteria; probiotic; pediococcus acidilactici

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DOI: https://doi.org/10.36987/jpbn.v10i2.5699

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