Hepatoprotective Activity of Hibiscus Flowers (Hibiscus rosa-sinensis) and Soursop Leaves (Annona muricata) Mix Infusion in Mus musculus Post Hyperuricemia Induction

Dian Fita Lestari, Vestidhia Yunisya Atmaja, Fatimatuzzahra Fatimatuzzahra, Shahnaz Shabrina Wulansari, Eliza Febrianti

Abstract


Background: Liver has many functions in the metabolic system and detoxification of substances that are harmful to the body. Chemical intoxication due to drug use might induce liver damage. A histology examination can indicate liver damage induced by a chemical substance, as well as the condition of tissue structure and function associated with the disease. This study aims to investigate the hepatoprotective effect of mix infusion of hibiscus flowers and soursop leaves on the liver histological structure in mice induced by hyperuricemia using potassium oxonate. Methodology: This study used an experimental group design, involving 8-week-old mice. The groups were divided as follows: A1 (control), A2 (Potassium oxonate), A3 (allopurinol), A4 (infusion 25 %), A5 (infusion 35 %), and A6 (infusion 45 %). Histological image was examined using an optilab with five fields of view. Findings: The study's revealed that the control group had a normal liver histology structure, whereas the hyperuricemia treatment group experienced significant liver damage, including congestion and leukocyte infiltration, and the allopurinol treatment group experienced significant damage, including leukocyte infiltration and necrosis. Therapy with a combined infusion of hibiscus flowers and soursop leaves shown a hepatoprotective effect. Based on histological analysis, the 25 % concentration provided the greatest hepatoprotective impact when compared to other infusion concentration. Mix infusion of hibiscus flowers and soursop leaves demonstrates heproprotective activity in hyperuricemia mice induced by potassium oxonate. Contribution: This study demonstrates that the mix infusion of hibiscus flowers and soursop leaves has the potential to be a hepatoprotective agent, but further research is needed regarding long-term effects


Keywords


Hepatoprotective; Hibiscus; Histology; Hyperuricemia; Infusion

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References


Abasa, S., & Ishak, P. (2022). Aktivitas Hepatoprotektor Ekstrak Etanol Kulit Buah Naga Merah (Hylocereus Polyrhizus) Terhadap Hepatotoksisitas Akut Paracetamol Pada Tikus (Rattus Norvegicus). Pharmacology And Pharmacy Scientific Journals, 1(1), 11–19. https://doi.org/10.51577/papsjournals.v1i1.304

Ali, M., Khan, T., Fatima, K., Ali, Q. ul A., Ovais, M., Khalil, A. T., Ullah, I., Raza, A., Shinwari, Z. K., & Idrees, M. (2018). Selected hepatoprotective herbal medicines: Evidence from ethnomedicinal applications, animal models, and possible mechanism of actions. Phytotherapy Research, 32(2), 199–215. https://doi.org/10.1002/ptr.5957

Ari Hepi Yanti, R. P. P. D. W. R. (2018). Aktivitas Hepatoprotektif Ekstrak Metanol Buah Lakum (Cayratia trifolia (L.) Domin) Terhadap Diameter Vena Sentralis, Lebar Sinusoid dan Berat Hepar Tikus Putih (Rattus novergicus L.) yang Diinduksi Parasetamol. Jurnal Protobiont, 7(3), 71–78. https://doi.org/10.26418/protobiont.v7i3.29088

Barnes, M. A., Roychowdhury, S., & Nagy, L. E. (2014). Innate immunity and cell death in alcoholic liver disease: Role of cytochrome P4502E1. Redox Biology, 2(1), 929–935. https://doi.org/10.1016/j.redox.2014.07.007

Burhan, A., Usmar, U., Zulham, Z., & Andarwiyati, A. (2018). The effect of kersen’s skin infusion (Muntingia calabura L.) on blood uric acid levels of the rats (Rattus novergicus). Jurnal Kedokteran Dan Kesehatan Indonesia, 9(3), 175–180. https://doi.org/10.20885/jkki.vol9.iss3.art7

Ebenyi, L. N. . (2012). A Comparision of the Effects of Aloe Barbadensis and Allium Sativum Extracts on Paracetamol – Induced Hepatotoxicity In Albino Rats. IOSR Journal of Pharmacy and Biological Sciences, 4(5), 28–31. https://doi.org/10.9790/3008-0452831

Farghali, H., Canová, N. K., & Zakhari, S. (2015). Hepatoprotective properties of extensively studied medicinal plant active constituents: Possible common mechanisms. Pharmaceutical Biology, 53(6), 781–791. https://doi.org/10.3109/13880209.2014.950387

Fatimatuzzahra, F., & Lestari, D. F. (2022). Potensi Infusa Bunga Kembang Sepatu (Hibiscus Rosa-Sinensis) Sebagai Anti Hiperurisemia Pada Mencit (Mus musculus). Jurnal Biosilampari : Jurnal Biologi, 4(2), 53–62. https://doi.org/10.31540/biosilampari.v4i2.1534

Fontana, Robert John; Li,Yi-Ju; Elizabeth Phillips, Naba Saeed, Huiman Barnhart, David Kleiner, J. H. (2021). Allopurinol hepatotoxicity is associated with HLA Class I Alleles. Liver International: Official Journal of the International Association for the Study of the Liver, 41(8), 1884–1893. https://doi.org/10.1111/liv.14903.Allopurinol

Hardiningtyas, S. D., Purwaningsih, S.-, & Handharyani, E.-. (2014). Aktivitas Antioksidan Dan Efek Hepatoprotektif Daun Bakau Api-Api Putih. Jurnal Pengolahan Hasil Perikanan Indonesia, 17(1), 80–91. https://doi.org/10.17844/jphpi.v17i1.8140

Ilkafah. (2018). Efektivitas Daun Sirsak dalam Menurunkan Nilai Asam Urat dan Keluhan Nyeri pada Penderita Gout di Kelurahan Tamalanrea Makassar. Pharmacon, 6(2), 22–29.

Irtanto, O., Pangkahila, A., & Aman, I. (2017). Pemberian Ekstrak Floret Pisang Raja (Musa x paradisiaca) Mencegah Penurunan Kadar Superoksida Dismutase (SOD) pada Hati Mencit (Mus musculus) BALB/c dengan Aktivitas Fisik Berlebih. Jurnal Biomedik (Jbm), 9(3), 166–171. https://doi.org/10.35790/jbm.9.3.2017.17338

Lestari, D. F., Fatimatuzzahra, Sianipar, A. P., Wulansari S.S. (2022). Allopurinol Induction on Histopathological Structure of the Liver in Male Mice (Mus musculus). Biodjati, 7(2), 280–291. https://doi.org/10.15575/biodjati.v7i2.18616

Oktavia, S., Ifora, I., Suhatri, S., & Susanti, M. (2017). Uji Aktivitas Hepatoprotektor Ekstrak Daun Sirih Hijau (Piper betle Linn.) Terhadap Kerusakan Hati Yang Diinduksi Parasetamol. Jurnal Farmasi Higea, 9(2), 109.

Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5. https://doi.org/10.1017/jns.2016.41

Riset Kesehatan Dasar. (2018). Laporan Riskesdas 2018 Nasional.pdf. In Lembaga Penerbit Balitbangkes (p. hal 156). https://repository.badankebijakan.kemkes.go.id/id/eprint/3514/1/Laporan Riskesdas 2018 Nasional.pdf. Accessed on December 20, 2024.

Tang, D. H., Ye, Y. S., Wang, C. Y., Li, Z. L., Zheng, H., & Ma, K. L. (2017). Potassium oxonate induces acute hyperuricemia in the tree shrew (Tupaia belangeri chinensis). Experimental Animals, 66(3), 209–216. https://doi.org/10.1538/expanim.16-0096

Ulusoy, E., & Eren, B. (2006). Histological changes of liver glycogen storage in mice (Mus musculus) caused by high-protein diets. Histology and Histopathology, 21(7–9), 925–930. https://doi.org/10.14670/HH-21.925

Wen, S., Wang, D., Yu, H., Liu, M., Chen, Q., Bao, R., Liu, L., Zhang, Y., & Wang, T. (2020). The time-feature of uric acid excretion in hyperuricemia mice induced by potassium oxonate and adenine. International Journal of Molecular Sciences, 21(15), 1–12. https://doi.org/10.3390/ijms21155178

Yuliawati, Y., Putri, W. C. W., & Rahman, H. (2021). Uji Aktivitas Hepatoprotektor Ekstrak Etanol Daun Rambutan (Nephelium lappaceum L.) pada Mencit Putih Jantan yang Diinduksi Parasetamol. Pharmacon: Jurnal Farmasi Indonesia, 18(2), 148–156. https://doi.org/10.23917/pharmacon.v18i2.15732

Zakiah, N., Yanuarman, Y., Frengki, F., & Munazar, M. (2017). Aktifitas Hepatoprotektif Ekstrak Etanol Daun Sirsak (Annona Muricata L.) Terhadap Kerusakan Hati Tikus yang Diinduksi dengan Parasetamol. AcTion: Aceh Nutrition Journal, 2(1), 25. https://doi.org/10.30867/action.v2i1.33

Zhou, Y., Wang, J., Zhang, D., Liu, J., Wu, Q., Chen, J., Tan, P., Xing, B., Han, Y., Zhang, P., Xiao, X., & Pei, J. (2021). Mechanism of drug-induced liver injury and hepatoprotective effects of natural drugs. Chinese Medicine (United Kingdom), 16(1), 2-32. https://doi.org/10.1186/s13020-021-00543-x

Zulkawi, N., Ng, K. H., Zamberi, R., Yeap, S. K., Jaganath, I. B., Satharasinghe, D., Yeah Yong, C., Jamaluddin, A. B., Tan, S. W., Ho, W. Y., Alitheen, N. B., & Long, K. (2017). The in vivo hepato-recovery effects of the polyphenol-rich fermented food XenijiTM on ethanol-induced liver damage. RSC Advances, 7(61), 38287–38299. https://doi.org/10.1039/c7ra04616b




DOI: https://doi.org/10.36987/jpbn.v11i2.6931

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