Biodegradation of Oil Palm Empty Fruit Bunch Waste by Pleurotus ostreatus and Volvariella volvacea Using Solid-State Fermentation

Umi Nur Jannah, Dewi Chusniasih, Erma Suryanti, Khaerunissa Anbar Istiadi

Abstract


Background: Oil palm empty fruit bunches (OPEFB) represent the largest form of solid waste generated from palm oil production, comprising approximately 23 % of the weight of every ton of fresh fruit bunches (FFB) processed. Untreated OPEFB waste causing pollution problems and increase toxicity due to methane emission. This study aims to evaluate the potential of OPEFB as a growth substrate for the cultivation of Pleurotus ostreatus and Volvariella volvacea, as well as to assess the reduction in lignocellulosic content following fungal biodegradation. Methodology: The experiment was conducted using solid-state fermentation (SSF), and lignocellulose content was analyzed using the Chesson method and SNI 0429:2008 through descriptive quantitative analysis. Findings: Over a 21-day incubation period, mycelial growth of both fungi successfully colonized the OPEFB baglogs, resulting in a 1–2 % reduction in baglog weight. Both P. ostreatus and V. volvacea demonstrated the ability to degrade lignocellulose by secreting lignin peroxidase (LiP), manganese peroxidase (MnP), laccase, cellulase, and hemicellulase enzymes. Initial lignocellulose levels of OPEFB were 18 % lignin, 57 % cellulose, and 20 % hemicellulose. After 21 days of incubation, P. ostreatus reduced these levels to 10 %  lignin, 47 % cellulose, and 19 % hemicellulose, while V. volvacea reduced them to 11 % lignin, 52 % cellulose, and 18 % hemicellulose. Contribution: These findings indicate that OPEFB is a viable substrate for mushroom cultivation and can be effectively biodegraded by these fungi, offering a sustainable approach to managing palm oil industry waste. The treated OPEFB can be used as organic fertilizer, animal feed, and briquettes


Keywords


Biodegradation; linocellulose; empty oil palm fruit bunches (OPEFB); Pleurotus ostreatus; Volvariella volvacea

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References


Alhidayatullah, ., Sudirman1, L. I., & Dharmaputra, O. S. (2014). The ability of JPA isolate wood-decaying fungi and Trichoderma Sp. S2-2 to degrade empty palm oil bunches to produce cellulose. E-Journal Menara Perkebunan, 82(2). https://doi.org/10.22302/iribb.jur.mp.v82i2.17 [In Indonesian language]

Andlar, M., Rezić, T., Marđetko, N., Kracher, D., Ludwig, R., & Šantek, B. (2018). Lignocellulose degradation: An overview of fungi and fungal enzymes involved in lignocellulose degradation. Engineering in Life Sciences, 18(11), 768–778. https://doi.org/10.1002/elsc.201800039

Anita, S. H., Hermiati, E., & Budi Laksana, R. P. (2016). The Effect of Preliminary Treatment with a Mixed Culture of White Rot Fungi Phanerochaete Crysosporium, Pleurotus Ostreatus, and Trametes Versicolor on the Lignin and Cellulose Content of Bagasse. Jurnal Selulosa, 1(02). https://doi.org/10.25269/jsel.v1i02.23 [In Indonesian language]

Badan Pusat Statistik. (2022). Smallholder Plantation Production by Crop Type in Lampung Province (thousand tonnes).. https://lampung.bps.go.id/id/statistics-table/3/Y0hOWWFGZHpPVkpUVjFKUlowVjBhMUI1Wm1aWFp6MDkjMw==/produksi-perkebunan-rakyat-menurut-jenis-tanaman-di-provinsi-lampung--ribu-ton---2022.html?year=2022. Accessed on August 14Th 2024 [In Indonesian language]

Bellettini, M. B., Fiorda, F. A., Maieves, H. A., Teixeira, G. L., Ávila, S., Hornung, P. S., Júnior, A. M., & Ribani, R. H. (2019). Factors affecting mushroom Pleurotus spp. Saudi Journal of Biological Sciences, 26(4), 633–646. https://doi.org/10.1016/j.sjbs.2016.12.005

Bilal, M., & Asgher, M. (2016). Biodegradation of agrowastes by lignocellulolytic activity of an oyster mushroom, Pleurotus sapidus. Journal of the National Science Foundation of Sri Lanka, 44(4), 399. https://doi.org/10.4038/jnsfsr.v44i4.8022

Chesson, A. (1981). Effects of sodium hydroxide on cereal straws in relation to the enhanced degradation of structural polysaccharides by rumen microorganisms. Journal of the Science of Food and Agriculture, 32(8), 745–758. https://doi.org/10.1002/jsfa.2740320802

Fadilah, F., Distantina, S., & Artati, E. K. (2008). Biodelignification of Corn Stalks Using Phanerochaete chrysosporium White Rot Fungi. Ekuilibrium. 7(1), 7–11. [In Indonesian language]

Hamzah, P., Syaifuddin, S., Rachmat, R., & Agus, A. (2022). Analysis of the Growth of F1 White Oyster Mushroom (Pleurotus ostreatus) Mycelium Using Corn and Rice Seeds as Media. JASATHP: Jurnal Sains dan Teknologi Hasil Pertanian, 2(2), 67–77. https://doi.org/10.55678/jasathp.v2i2.807 [In Indonesian language]

Handayani, T., & Purwantisari, S. (2015). Isolation and Identification of Mold Contaminants on Mushroom Growing Medium (Bag Log) and Their Cellulolytic Performance Test. Jurnal Sains dan Matematika. 23(2), 55–58.

Hanif, H., Panji, T.-, Dimawarnita, F., & Artika, I. M. (2019). Purification of alpha-cellulose from ex-baglog of white oyster mushroom (Pleurotus ostreatus) using NaOH and sulfate hydrolysis. E-Journal Menara Perkebunan, 87(1), 52–59. https://doi.org/10.22302/iribb.jur.mp.v87i1.325 [In Indonesian language]

Hartari, W. R., Delvitasari, F., Maryanti, M., Undadraja, B., Hasbullah, F., & Deksono, G. A. (2023). Testing of Palm Oil Empty Fruit Bunch Lignocellulose with H2SO4 Delignification Time Using Pressurised Steam. Jurnal Agro Industri Perkebunan, 11(3), 151–158. https://doi.org/10.25181/jaip.v11i3.3007 [In Indonesian language]

Herliyana, E. N., Nandika, D., Ahmad,. Sudirman, L.I., & Witarto, A. B. (2008). Biodegradation of Sengon Wood Sawdust Substrate by Pleurotus Fungi from Bogor. J. Tropical Wood Science and Technology, 6(2), 75–84. [In Indonesian language]

Hoa, H. T., & Wang, C.-L. (2015). The Effects of Temperature and Nutritional Conditions on Mycelium Growth of Two Oyster Mushrooms ( Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology, 43(1), 14–23. https://doi.org/10.5941/MYCO.2015.43.1.14

Hunaepi, H., Dharawibawa, I. D., Asy’ari, M., Samsuri, T., & Mirawati, B. (2018). Processing Oyster Mushroom Baglog Waste into Commercial Organic Fertiliser. Jurnal SOLMA, 7(2), 277. https://doi.org/10.29405/solma.v7i2.1392 [In Indonesian language]

Iksan, A. (2015). Delignification of White Teak Wood Powder (Gmelina arborea Roxb.) Using Gamma-Irradiated Phanerochaete chrysosporium Fungi. Undergraduate thesis of Universitas Islam Negeri Syarif Hidayatullah. http://repository.uinjkt.ac.id/dspace/handle/123456789/43442. Accessed: August 14th 2024 [In Indonesian language]

Imsya, A., Laconi, E. B., Wiryawan, K. G., & Widyastuti, Y. (2014). Biodegradation of lignocellulose by Phanerochaete chrysosporium on changes in the nutritional value of palm fronds. Jurnal Peternakan Sriwijaya, 3(2). https://doi.org/10.33230/JPS.3.2.2014.1762 [In Indonesian language]

Khadafi, M. I., & Fordini, S. B. (2023). The Effect of Acid Catalyst Types in the Hydrolysis of Sweet Sorghum Plant Stems. Prosiding Seminar Nasional Soebardjo Brotohardjono XIX, 19(1), 163–168. [In Indonesian language]

Krishaditersanto, R. (2018). Degradation of sawdust fibre components resulting from the bioconversion of white oyster mushrooms (Pleurotus ostreatus) with different urea levels. Jurnal Ilmu-Ilmu Peternakan, 28(2), 175. https://doi.org/10.21776/ub.jiip.2018.028.02.10 [In Indonesian language]

Kurniawan, F. A., & Syukron, A. A. (2019). Characteristics of Biochar Briquettes from a Mixture of Oyster Mushroom (Pleurotus Ostreatus) Waste and Rice Husk. Indonesian Journal Of Applied Physics, 9(02), 76. https://doi.org/10.13057/ijap.v9i2.34478 [In Indonesian language]

Kusumaningrum, I., Zakia, N., & Nilasari, C. (2017). The Effect of Planting Medium Acidity (pH) and Harvest Time on Selenium Fortification in White Oyster Mushrooms (Pleurotus ostreatus). JC-T (Journal Cis-Trans): Jurnal Kimia Dan Terapannya, 1(1), 30–34. https://doi.org/10.17977/um026v1i12017p030 [In Indonesian language]

Lisin, N., & Hutomo, G. S. (2015). Hydrolysis of Cellulose from Cocoa Pod Husk Using Sulphuric Acid. e-J. Agrotekbis, 3(4), 482–490. [In Indonesian language]

Neville, F., Ardianto, R., Viktaria, V., Budihalim, V., & Sari, I. J. (2018). The effect of light intensity and sucrose content on the growth of oyster mushrooms in South Tangerang. Biodidaktika, jurnal biologi dan pembelajarannya, 13(2). https://doi.org/10.30870/biodidaktika.v13i2.3678 [In Indonesian language]

Ratnawati, D., Adfa, M., & Widiyati, E. (2018). Production of Briquettes and Compost from Palm Kernel Shells and Empty Fruit Bunches in Giri Mulya Village, North Bengkulu. Dharma Raflesia : Jurnal Ilmiah Pengembangan Dan Penerapan IPTEKS, 15(2). https://doi.org/10.33369/dr.v15i2.4056 [In Indonesian language]

Safitri, L. (2023). The Potential of Phanerochaete chrysosporium and Pleurotus ostreatus White Rot Fungi in the Degradation of Corn Cob Waste. Undergraduate thesis of Institut Teknologi Sumatera. https://repo.itera.ac.id/depan/submission/SB2301160074. Accessed: July 24th 2024 [In Indonesian language]

Santosa, S. J. (2008). Palm Oil Boom in Indonesia: From Plantation to Downstream Products and Biodiesel. CLEAN – Soil, Air, Water, 36(5–6), 453–465. https://doi.org/10.1002/clen.200800039

Selman, M. A. K. (2021). Delignification of Lignocellulosic Biomass Using Enzymes from Cossus-cossus Larvae in Second-Generation Biofuel Production. Undergraduate thesis of Universitas Islam Negeri Alauddin Makassar. https://repositori.uin-alauddin.ac.id/17976/. Accessed: July 20th 2024 [In Indonesian language]

Sienita, T. (2021). Utilisation of Cotton Waste from the Spinning Division of PT. Sri Rejeki Isman Tbk as a Base Material for White Oyster Mushroom (Pleurotus ostreatus) Growing Media. Undergraduate thesis of Universitas Gadjah Mada. https://etd.repository.ugm.ac.id/penelitian/detail/205009. Accessed: July 5th 2024 [In Indonesian language]

Suryadi, H., Judono, J. J., Putri, M. R., Eclessia, A. D., Ulhaq, J. M., Agustina, D. N., & Sumiati, T. (2022). Biodelignification of lignocellulose using ligninolytic enzymes from white-rot fungi. Heliyon, 8(2), e08865. https://doi.org/10.1016/j.heliyon.2022.e08865

Villas-Bôas, S. G., Esposito, E., & Mitchell, D. A. (2002). Microbial conversion of lignocellulosic residues for production of animal feeds. Animal Feed Science and Technology, 98(1–2), 1–12. https://doi.org/10.1016/S0377-8401(02)00017-2

Wang, F., Xu, L., Liting Zhao, Ding, Z., Ma, H., & Terry, N. (2019). Fungal Laccase Production from Lignocellulosic Agricultural Wastes by Solid-State Fermentation: A Review. Microorganisms, 7(12), 665. https://doi.org/10.3390/microorganisms7120665

Yenie, E., & Utami, S. P. (2017). The Effect of Temperature and pH on the Growth of Straw Mushrooms (Volvariella Volvacea) on the Degradation of Lignin in Empty Palm Fruit Bunches. Jurnal Fakultas Teknik Universitas Pasir Pengaraian. 10, 29–35. [In Indonesian language]




DOI: https://doi.org/10.36987/jpbn.v11i2.7327

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