Exploration Of Rice Plant Endophyte Bacteria To Suppress Xanthomonas oryzae Bacterial Leaf Blight Disease

Ratih Nadhila Putri, Yenny Wuryandari, Endang Tri Wahyu

Abstract


Background: Rice is a major food commodity for Indonesian. According to BPS (2022) rice production in 2021 decreased 0.43%. The decline caused by various factors, one of which is bacterial leaf blight disease by the pathogenic bacteria Xanthomonas oryzae. Control of X. oryzae mostly uses pesticides, but this control has a negative impact on the environment, therefore it is necessary to develop endophytic bacteria (EB) that can inhibit the growth of X. oryzae and can act as PGPR. This study was to determine the EB ability in healthy rice stem tissue to suppress bacterial leaf blight disease caused by the bacteria X. oryzae. Methodology: This research was were used isolation from infected plant for X. oryzae and from healthy plant for EB, data was analyzed using the corresponding formula. Findings: found 10 isolates of EB that could inhibit the X. oryzae growth as seen from the inhibition zone produced in the antagonist test. The EB isolates produced the largest inhibition zones such as BE18 (23.3 mm, very strong category), BE16 (13.3 mm, strong category), and BE3 (11.6 mm, strong category). The 4 isolates with bactericidal inhibition mechanisms, namely isolates (BE11, BE15, BE16, and BE18), 6 isolates with bacteriostatic inhibition mechanisms, namely isolates (BE3, BE5, BE6, BE7, BE12, and BE13). Contribution: The availability of EB present in the tissues of healthy rice stems is still relatively high and can be used to suppress the growth of pathogenic bacterium X. oryzae. This rseult also supported by the beneficial properties of EB, which can enhance plant resistance


Keywords


Endophyte bacteria; Rice plant; X. oryzae

Full Text:

PDF

References


Alan, Y., Nurcahyanti SD, Addy HS. (2016). The potential of biological agents in suppressing the development of bacterial leaf blight (Xanthomonas oryzae Pv. Oryzae) in paddy. J. Agrotek. Trop, 5(2), 70–76. http://repository.unej.ac.id/handle/123456789/78917 [In Indonesian language]

Ali, M.A., Ahmed, T., Ibrahim, E., Rizwan, M., Chong, K.P., Yong, J.W.H. (2024). A review on mechanisms and prospects of endophytic bacteria in biocontrol of plant pathogenic fungi and their plant growth-promoting activities. Heliyon, 10(11), e31573, 14p. https://doi.org/10.1016/j.heliyon.2024.e31573

Badan Pusat Statistik [BPS]. (2020). Crop Area and Rice Production in 2020. https://www.bps.go.id/id/pressrelease/2020/10/15/1757/luas-panen-dan-produksi-padi-pada-tahun-2020-mengalami-kenaikan-dibandingkan-tahun-2019-masing-masing-sebesar-1-02-dan-1-02-persen-.html. Accessed on 31Th Juli 2024. [In Indonesian language]

Desriani, D., Safira, U. M., Bintang, M., Rivai, A., & Lisdiyanti, P. (2014). Isolation and characterisation of endophytic bacteria from binahong and Chinese katepeng plants. Jurnal Kesehatan Andalas, 3(2) 89-93. https://doi.org/10.25077/jka.v3i2.33 [In Indonesian language]

Dewi, R. S., Kadir, T. S., & Nuryanto, B. 2015. Detection of Xanthomonas oryzae pv. oryzae seed infection and the relationship between disease severity and infection levels in paddy seeds. Balai Besar Penelitian Tanaman Padi 44, 449-158. https://repository.pertanian.go.id/handle/123456789/19795 [In Indonesian language]

Handoyo, B., Herlinawati. dan Soelaksini, L., 2018. Application of salt (NaCl) to increase the production of Situ Bagendit variety rice (Oryza sativa L.) in Banyuwangi lithosol soil. Agritrop, 16(2), 197 – 204. https://doi.org/10.32528/agritrop.v16i2.1803 [In Indonesian language]

Handayani, P. (2015). Isolation, Selection, and Antimicrobial Activity Testing of Endophytic Fungi from Jamblang Plant Leaves (Syzygium Cuminil.) Against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Candida albicans, and Aspergillus niger. Bachelor thesis of Fakultas Kedokteran dan Ilmu Kesehatan UIN Syarif Hidayatullah Jakarta. Accessed on 14Th Mei 2025 [In Indonesian language]

Hartanti, D. A. S. (2020). Isolation and synergy testing of endophytic bacteria in rice plants (Oryza sativa L.) for biofertiliser consortiums. Agroradix: Jurnal Ilmu Pertanian, 3(2), 23-30. DOI : https://doi.org/10.52166/agroteknologi.v3i2.1951 [In Indonesian language]

Iqlima, D., Ardiningsih, P., & Wibowo, M. A. (2017). Antibacterial activity of endophytic bacterial isolate B2D from the stem of the yacon plant (Smallanthus sonchifolius (Poepp. & Endl.) H. Rob.) against Staphylococcus aureus and Salmonella thypimurium bacteria. Jurnal Kimia Khatulistiwa, 7(1), 115-122. DOI: https://doi.org/10.31932/jpbio.v7i1.1522 [In Indonesian language]

Jamilatun, M., Aminah, A., & Shufiyani, S. 2020. Anti-bacterial inhibition test of endophytic fungi from Imperata cylindrica (L.) Beauv. against the growth of Staphylococcus aureus and Escherichia coli bacteria. Jurnal Medikes (Media Informasi Kesehatan), 7(2), 335-346. [In Indonesian language]

Khan, A. Jilani A., Jilani, G., Akhtar, M. S., Naqvi, S. M. S., dan Rasheed, M. 2009. Phosphorus solubilizing bacteria:occurrence,mechanisms and their role in crop production. Journal Agriculture Biol. Sci., 1(1), 48-58.

Kurniawati, S., & Mutaqin, K. H. 2015. Exploration and testing of bioactive compounds from biological agents to control sheath blight disease in paddy plant. Jurnal Hama dan Penyakit Tumbuhan Tropika, 15(2), 170-179. DOI: https://doi.org/10.23960/j.hptt.215170-179 [In Indonesian language]

Parida, I., Damayanti, T. A., & Giyanto, G. (2016). Isolation, selection, and identification of endophytic bacteria as agents inducing rice resistance to bacterial leaf blight. Jurnal Fitopatologi Indonesia, 12(6), 199-199. DOI: https://doi.org/10.14692/jfi.12.6.199 [In Indonesian language]

Pradana, A. P., Putri, D., & Munif, A. (2015). Exploration of endophytic bacteria from the roots of Adam and Eve plants and their potential as biological agents and growth promoters for rice plants. Jurnal Fitopatologi Indonesia, 11(3), 73-73. DOI: https://doi.org/10.14692/jfi.11.3.73 [In Indonesian language]

Tjiptoningsih, U. G. 2020. Evaluation of the water-resistant properties of lemon juice (Citrus limon (L.) Burm. F.) against the growth of Aggregatibacter actinomycetemcomitans bacteria. Jurnal Ilmiah dan Teknologi Kedokteran Gigi, 16(2), 86-96. DOI: https://doi.org/10.32509/jitekgi.v16i2.1100 [In Indonesian language]

Srinivas, B., Patil, V. A., Venu, E., Hariprasath, M., Purushotham, P., Rajeswari, E & Rajesh, K. 2024. Isolation, purification and identification of Xanthomonas oryzae pv. oryzae. International Journal of Economic Plants, 11(Feb, 1), 032-037. doi: https://doi.org/10.23910/2/2024.5076c

Serdani, A. D., Aini, L. Q., & Abadi, A. L. (2018). Isolation and identification of endophytic bacteria from rice plants (Oryza sativa) as a control agent for bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae. Viabel: Jurnal Ilmiah Ilmu-Ilmu Pertanian, 12(1), 18-26. https://doi.org/10.35457/viabel.v12i1.422 [In Indonesian language]

Wahyudi, A. T., Meliah, S., & Nawangsih, A. A. (2011). Xanthomonas oryzae pv. oryzae, the bacterium that causes leaf blight in rice: isolation, characterisation, and mutagenesis analysis with transposons. Makara Journal of Science, 15(1), 35. https://scholarhub.ui.ac.id/science/vol15/iss1/35 [In Indonesian language]

Zafar, S., & Jianlong, X. (2023). Recent Advances to Enhance Nutritional Quality of Rice. Rice Science, 30(6), 523-536. https://doi.org/10.1016/j.rsci.2023.05.004

Zinidin, M. 2022. Exploration of Bacillus spp. in the Rhizosphere of Red Chili (Capsicum annuum L.) in Highlands and Its Potential as a Biological Agent Against Ralstonia solanacearum Pathogens in Vitro. Doctoral dissertation of UPN" Veteran" Jawa Timur.

http://repository.upnjatim.ac.id/id/eprint/6876 [In Indonesian language]




DOI: https://doi.org/10.36987/jpbn.v11i2.7338

Refbacks

  • There are currently no refbacks.


Copyright (c) 2025 Ratih Nadhila Putri

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Jurnal Pembelajaran dan Biologi Nukleus (JPBN) by LPPM Universitas Labuhanbatu is under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY - SA 4.0). Official contact: Rivo +6281362238917