Synergystic Role of Cocopeat Matrix and Foliar Nutrient Type on Aclimatization Success of Micropropagated Dendrobium Seedlings

Baharuddin Mohammad Luthfi, Ramdan Hidayat, Pangesti Nugrahani

Abstract


Background: Dendrobium orchids have high economic value (in 2020 generated an export value of US$61.87 thousand), yet micropropagated seedlings commonly exhibit slow growth and high mortality during the acclimatization stage. This problem is largely associated with the use of unsuitable planting media and inadequate nutrient supply, which limit seedling adaptation and survival after tissue culture. This study aims to determine the best combination of planting media and foliar fertilizer types for the growth of Dendrobium orchid seedlings during the acclimatization stage. Methodology: The study was conducted in Penambangan Village, Semanding District, Tuban Regency, East Java Province from November 2024 to February 2025 using a Completely Randomized Design (CRD) with 9 treatment combinations and 4 replications. The first factor was the treatment of planting media types (M1: Black Moss, M2: Rice Husk Charcoal, M3: Cocopeat), and the second factor was the type of foliar fertilizer (P1: Gandasil D, P2: Gaviota 63, P3: Grow Quick LB). The observed research variables included the percentage of live seedlings, plant height, leaf area, seedling growth rate, number of shoots, and number of roots. Findings: The results showed that the use of cocopeat planting media resulted in the highest percentage of seedling survival at 88.33%. The combination of cocopeat planting media and Grow Quick LB leaf fertilizer produced the best relative growth rate (RGR) at 8-12 WAP of 41.22 mg/month.This research produces the best combination of planting media and foliar fertilizer for orchid seedlings at the acclimatization stage. Contribution: The findings contribute to a more systematic understanding of how substrate physical properties and foliar nutrient supply interact to influence survival and growth performance during orchid acclimatization, offering a scientifically grounded framework for improving ex vitro propagation efficiency


Keywords


Acclimatization; Dendrobium Orchids; Planting media; Foliar fertilizer

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References


Agustiar, R. D., Trisnaningsih, U., & Wahyuni, S. (2021). P The Effect of Various Planting Media Compositions and Leaf Fertiliser Concentrations on the Growth of Dendrobium Orchid Seedlings (Dendrobium sp.). Agroswagati Jurnal Agronomi, 8(2), 52-57. https://doi.org/10.33603/agroswagati.v8i2.4944 [In Indonesian language]

Ali, F. Y., Rohman, H. F., Sukri, M. Z., Rosdiana, E., Saputri, E. D., & Bulan, A. (2024). The Effect of Providing Foliar Fertilizer on the Acclimatized Growth of Anggrek bulan ( Phalaenopsis amabilis Var . Aurea ). Jurnal Agrium, 21(2), 165-170. [In Indonesian language]

Andalasari, T. D., Yafisham, Y., & Nuraini, N. (2014). Growth Response of Dendrobium Orchids to Types of Planting Media and Leaf Fertiliser. Jurnal Penelitian Pertanian Terapan, 14(3), 167–173. https://doi.org/10.25181/jppt.v14i3.156 [In Indonesian language]

Andiani, Y. (2018). Orchid Nursery Business in Bottles. Yogyakarta: Pustaka Baru Press. [In Indonesian language]

Anhar, T., Respatie, D. W., & Purwantoro, A. (2022). Study of the Growth and Yield of Five Green Bean Accessions (Vigna radiata L.). Vegetalika, 11(4), 292. https://doi.org/10.22146/veg.74390 [In Indonesian language]

Astutik., Sumiati, A., Agastha, I. M. I., Sutoyo. (2022). The Use of Several Types of Leaf Fertiliser and Container Materials During the Flowering Phase of Phalaenopsis sp. Jurnal Buana Sains, 22(2), 7-14. https://doi.org/10.33366/bs.v22i2.3744 [In Indonesian language]

Aulia, S., Emalya, M. M., & Kurniawati, H. (2025). PThe Effect of Variations in the Application of Gaviota 63 and Gandasil D Foliar Fertilisers on the Growth of Dendrobium Orchids in the Seedling Phase. Prosiding Seminar Nasional Sains Dan Teknologi Seri III Fakultas Sains Dan Teknologi, Universitas Terbuka, 2(1), 1069–1080. [In Indonesian language]

Bani, R., Dewanti, P., Restanto, D. P., Widuri, L. I., & Alfian, F. N. (2022). The Application of Chitosan on the Growth of Dendrobium Sonia Orchids During the Acclimatisation Stage. Jurnal Penelitian Pertanian Terapan, 22(2), 146–154. https://doi.org/10.25181/jppt.v22i2.2264 [In Indonesian language]

Derantika, C., Jurusan, E. N., Pertanian, B., & Pertanian, F. (2018). The Effect of Water Supply and Nitrogen Fertilizer on Growth Pennywort (Centella asiatica L.Urb). Plantropica Journal of Agricultural Science, 3(2), 78–84. [In Indonesian language]

Erfa, L., Maulida, D., Sesanti, R. N., & Yuriansyah, Y. (2020). The Success of Acclimatisation and Seedling Cultivation of Moon Orchids (Phalaenopsis) in Several Growing Medium Combinations. Jurnal Penelitian Pertanian Terapan, 19(2), 122. https://doi.org/10.25181/jppt.v19i2.1420 [In Indonesian language]

Ezperanza, P., Suryadi, E., & Amaru, K. (2023). The Use of Charcoal, Cocopeat and Zeolite Planting Media Composition in Drip Irrigation Systems on Melon Plant Growth and Yield. Journal of Integrated Agricultural Socio-Economics and Entrepreneurial Research (JIASEE), 1(2), 19. https://doi.org/10.26714/jiasee.1.2.2023.19-24 [In Indonesian language]

Haniva, A., Hidayati, S., & Farid, N. (2020). The Effect of Planting Media Types and Varieties on the Growth of Dendrobium Orchids in a Drip Irrigation System. Proceeding of Senaster "Seminar Nasional Riset Teknologi Terapan 2020, 1, 1-8. https://jurnal.untidar.ac.id/index.php/senaster/article/view/2674%0A [In Indonesian language]

Herliana, O., Rokhminarsi, E., Mardini, S., & Jannah, M. (2018). The effect of growing media type and application of mycorrhizal biofertiliser on growth, flowering and mycorrhizal infection in Dendrobium sp. orchids. Kultivasi, 17(1), 550–557. https://doi.org/10.24198/kultivasi.v17i1.15774 [In Indonesian language]

Indiriani, E., Tini, E. W., dan Djatmiko, H. A. (2019). Different Types of Planting Media and Leaf Fertiliser Concentrations. Agrin, 23(1), 24–33. http://dx.doi.org/10.20884/1.agrin.2019.23.1.429 [In Indonesian language]

Irsyadi, M. B., & Wulanjari, D. (2023). Growth Response of Cattleya Orchid Seedlings (Cattleya sp.) Hybrids to Media Type and Leaf Fertiliser Concentration During the Acclimatisation Stage. Agribios, 21(2), 157. https://doi.org/10.36841/agribios.v21i2.3608 [In Indonesian language]

Ivani, N., & Wahyuni, E. S. (2024). The Effect of NPK Fertiliser Composition and Photosynthetic Bacteria Concentration on the Growth and Development of Moon Orchids (Phalaenopsis amabilis). Jurnal Bioshell, 13(2), 117–122. [In Indonesian language]

Kaveriamma, M. M., Rajeevan, P. K., Girija, D., & Nandini, K. (2019). Sphagnum Moss as Growing Medium in Phalaenopsis Orchid. International Journal of Current Microbiology and Applied Sciences, 8(02), 2118–2123. https://doi.org/10.20546/ijcmas.2019.802.245

Kismayanti, C. N., Ulfah, M., & Rachmawati, R. C. (2023). Growth Response of Dendrobium Orchids in the Seedling Phase to Variations in Planting Media Types. Bioscientist : Jurnal Ilmiah Biologi, 11(2), 1563. https://doi.org/10.33394/bioscientist.v11i2.9368 [In Indonesian language]

Latif, I. N., Siswandi, Dewi, R. N. Nurul, I., Siswadi, L., & Ratna, D. (2024). Testing the Concentration of Liquid Organic Fertiliser and Leaf Fertiliser on Orchid Growth (Dendrobium sp). Innofarm: Jurnal Inovasi Pertanian, 26(2), 170–174. [In Indonesian language]

Maera, Z. (2015). Growth Response of Phalaenopsis Hybrid Orchid Planlets To Two Foliar Fertilizer and Benzyladenin At Aclimatization Stage. Jurnal Enviagro, 7(2), 1–6. [In Indonesian language]

Mirani, A.A., Adel A. A., Ghulam S. M. (2017). Effect Of Different Substrates On Survival And Growth Of Transplanted Orchids (Dendrobium Nobile Cv.) Into Net House Gene Clonning View Project Genetic Stability Of Micropropagated Date Palms View Project Effect Of Different Substrates On Survival And Gro. International Journal of Horticulture and Floriculture, 5(4), 310–317. https://www.researchgate.net/publication/337464461

Nadhiroh, L. A., Herastuti, H., & Setyaningrum, T. (2022). The Use of Various Types of Leaf Fertiliser and Growing Media on Dendrobium sp Orchids. Agrivet, 28(1), 27. https://doi.org/10.31315/agrivet.v28i1.6028 [In Indonesian language]

Ningsih, R., Nugroho, S. E. ., & Cahyono, E. H. (2025). Application of Several Types of Fertilizer for Moon Orchid Acclimatization (Phalaenopsis Amabilis) Tissue Culture Results. Jurnal Pengembangan Potensi Laboratorium, 3(2), 69–78. https://doi.org/10.25047/plp.v3i2.4521 [In Indonesian language]

Nugroho, C., & Raden, I. (2021). Acclimatisation of Three Types of Orchids in Different Growing Media. Jurnal Pertanian, 12(2), 109–117. [In Indonesian language]

Nuzullah, A. F, & Firgiyanto, R. (2021). Increasing Agricultural Productivity in the Post-Pandemic Society 5.0 Era Application of Various Types of Media and ZPT to the Acclimatisation of Vanda Orchids (Vanda sp.). Agropross, Proceeding of National Conference of Agriculture, Politeknik Negeri Jember 2021, 10–24. https://doi.org/10.25047/agropross.2021 [In Indonesian language]

Pusat Data dan Sistem Informasi Pertanian Kementrian Pertanian. (2020). Outlook for Orchids in the Horticultural Sub-sector of Agricultural Commodities. Pusat Data dan Sistem Informasi Pertanian Sekretariat Jenderal Kementerian Pertanian 2020. 58. [In Indonesian language]

Putri, A. V., Rahayu, A. P., & Wardiyati, T. (2022). The Effect of Planting Media and Foliar Fertiliser on the Acclimatisation of Dendrobium Orchid Seedlings (Dendrobium sp.). Jurnal Produksi Tanaman, 10(8), 451–457. [In Indonesian language]

Sucandra, A., Silvina, F., & Yulia, A. E. (2015). Testing the Effect of Several Concentrations of Glycine in Vacciniola and Went (VW) Media on the In Vitro Growth of Orchid Plantlets (Dendrobium Sp.). Jom Faperta, 2(1), 1–11. https://jom.unri.ac.id/index.php/JOMFAPERTA/article/view/5849 [In Indonesian language]

Surtinah, & Mutryarny, E. (2013). Frequency of Grow Quick LB Application on the Growth of Dendrobium Orchid Seedlings in the Pot Community Stage. Jurnal Ilmiah Pertanian, 10(2), 31–40. [In Indonesian language]

Suyanto, A., Setiawan., & Ropiana, K. (2021). Utilisation of Various Types of Growing Media for the Growth of Moon Orchids (Phalaenopsis amabilis) in Individual Pots. Agrofood Jurnal Pertanian Dan Pangan, 3(2), 22–27. https://doi.org/10.63848/agf.v03n2.3 [In Indonesian language]

Widiastoety, D., Solvia, N., & Soedarjo, M. (2010). The Potential of Dendrobium Orchids in Increasing the Variety and Quality of Cut Orchids. Jurnal Litbang Pertanian, 29 (3), 101–106. [In Indonesian language]




DOI: https://doi.org/10.36987/jpbn.v11i4.8174

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