Acute–Chronic Response Continuum Indicates Physiological Trajectories in Daphnia Under Salinity Stress

Yadi Oktariansyah, Arum Setiawan, Sri Maulina, Ferta Liza Putri, M. Wahyu Sobirin

Abstract


Background: Freshwater salinization is an emerging global stressor that disrupts osmotic regulation and energy balance in aquatic invertebrates. Although acute and chronic toxicity are traditionally evaluated separately, their biological relationship remains insufficiently integrated. This study aimed to determine whether salinity exposure in a laboratory-maintained population of Daphnia sp. is expressed as an acute–chronic response continuum, with physiological trajectories emerging across survival and reproductive endpoints. Methodology: Acute toxicity was assessed through a 24-hour sodium chloride exposure (0.00–3.80 g/L) to determine the LC50, followed by a 10-day chronic experiment using fractions of the LC50 (0–20%). Survival probability and reproductive onset were analyzed using Kaplan–Meier estimation and Cox proportional hazards models, while offspring production was evaluated using non-parametric tests. Findings: The 24-hour LC50 was estimated at 1.87 g/L. Chronic exposure significantly altered survival dynamics (χ² = 11.7, p = 0.02) and reproductive onset (χ² = 10.3, p = 0.04). Reproductive success declined from 100% in controls to 20–40% in exposed groups, while total offspring production differed significantly among treatments according to the Kruskal–Wallis test (χ² = 9.74, p = 0.045) and the Jonckheere–Terpstra test indicated a significant decreasing trend across the salinity gradient (JT = 79.5, p = 0.0148). Sublethal reproductive impairment occurred at concentrations far below the acute lethal threshold. Contributions: Salinity stress in Daphnia sp. operates along a continuous and time-dependent physiological trajectory, where reproductive disruption precedes mortality. Integrating acute lethality with chronic survival and reproductive responses provides a more ecologically relevant framework for freshwater salinity risk assessment


Keywords


Acute–Chronic Continuum; Daphnia; Kaplan–Meier Analysis; Physiological Trajectory; Salinity Stress

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Asharam, K., Mitku, A. A. A., Ramsay, L., Jeena, P. M., & Naidoo, R. N. (2024). Environmental exposures associated with early childhood recurrent wheezing in the mother and child in the environment birth cohort: a time-to-event study. Thorax, 79(10), 953–960. https://doi.org/10.1136/thorax-2023-221150

Barrett, N. J., Harper, E. M., Last, K. S., Reinardy, H. C., & Peck, L. S. (2024). Behavioural and physiological impacts of low salinity on the sea urchin Echinus esculentus. Journal of Experimental Biology, 227(2), jeb246707. https://doi.org/10.1242/jeb.246707

Cochran, J. K., Banks, C., & Buchwalter, D. B. (2023). Respirometry reveals major lineage-based differences in the energetics of osmoregulation in aquatic invertebrates. Journal of Experimental Biology, 226(20), jeb246376. https://doi.org/10.1242/jeb.246376

Cribiu, P., Devaux, A., Garnero, L., Abbaci, K., Bastide, T., Delorme, N., Quéau, H., Degli Esposti, D., Ravanat, J.-L., Geffard, O., Bony, S., & Chaumot, A. (2020). A “Population Dynamics” Perspective on the Delayed Life-History Effects of Environmental Contaminations: An Illustration with a Preliminary Study of Cadmium Transgenerational Effects over Three Generations in the Crustacean Gammarus. International Journal of Molecular Sciences, 21(13), 4704. https://doi.org/10.3390/ijms21134704

Cunillera-Montcusí, D., Beklioğlu, M., Cañedo-Argüelles, M., Jeppesen, E., Ptacnik, R., Amorim, C. A., Arnott, S. E., Berger, S. A., Brucet, S., Dugan, H. A., Gerhard, M., Horváth, Z., Langenheder, S., Nejstgaard, J. C., Reinikainen, M., Striebel, M., Urrutia-Cordero, P., Vad, C. F., Zadereev, E., & Matias, M. (2022). Freshwater salinisation: a research agenda for a saltier world. Trends in Ecology & Evolution, 37(5), 440–453. https://doi.org/10.1016/j.tree.2021.12.005

Evans, T. G., & Kültz, D. (2020). The cellular stress response in fish exposed to salinity fluctuations. Journal of Experimental Zoology Part A: Ecological and Integrative Physiology, 333(6), 421–435. https://doi.org/10.1002/jez.2350

Hansul, S., Fettweis, A., Smolders, E., & Schamphelaere, K. De. (2024). Extrapolating Metal (Cu, Ni, Zn) Toxicity from Individuals to Populations Across Daphnia Species Using Mechanistic Models: The Roles of Uncertainty Propagation and Combined Physiological Modes of Action. Environmental Toxicology and Chemistry, 43(2), 338–358. https://doi.org/10.1002/etc.5782

Huang, J., Li, Y., Sun, Y., Zhang, L., Lyu, K., & Yang, Z. (2022). Size-specific sensitivity of cladocerans to freshwater salinization: Evidences from the changes in life history and population dynamics. Environmental Pollution, 296, 118770. https://doi.org/10.1016/j.envpol.2021.118770

Kassambara, A., Kosinski, M., & Biecek, P. (2016). survminer: Drawing Survival Curves using “ggplot2.” In CRAN: Contributed Packages. https://doi.org/10.32614/CRAN.package.survminer

Kaushal, S. S., Likens, G. E., Pace, M. L., Reimer, J. E., Maas, C. M., Galella, J. G., Utz, R. M., Duan, S., Kryger, J. R., Yaculak, A. M., Boger, W. L., Bailey, N. W., Haq, S., Wood, K. L., Wessel, B. M., Park, C. E., Collison, D. C., Aisin, B. Y. ’aaqob I., Gedeon, T. M., … Woglo, S. A. (2021). Freshwater salinization syndrome: from emerging global problem to managing risks. Biogeochemistry, 154(2), 255–292. https://doi.org/10.1007/s10533-021-00784-w

Lambret, P., Janssens, L., & Stoks, R. (2021). The impact of salinity on a saline water insect: Contrasting survival and energy budget. Journal of Insect Physiology, 131, 104224. https://doi.org/10.1016/j.jinsphys.2021.104224

Landes, J., Engelhardt, S. C., & Pelletier, F. (2020). An introduction to event history analyses for ecologists. Ecosphere, 11(10), e03238. https://doi.org/10.1002/ecs2.3238

Lee, S., Haque, Md. N., Lee, D.-H., & Rhee, J.-S. (2023). Comparison of the effects of sublethal concentrations of biofoulants, copper pyrithione and zinc pyrithione on a marine mysid - A multigenerational study. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 271, 109694. https://doi.org/10.1016/j.cbpc.2023.109694

Mantovani, M., & McNamara, J. C. (2021). Contrasting strategies of osmotic and ionic regulation in freshwater crabs and shrimps: gene expression of gill ion transporters. Journal of Experimental Biology, 224(3), jeb233890. https://doi.org/10.1242/jeb.233890

Mikulski, A., & Mazurczak, D. (2023). Maternal effect in salinity tolerance of Daphnia–One species, various patterns? PLOS ONE, 18(4), e0283546. https://doi.org/10.1371/journal.pone.0283546

Morris, C., & O’Donnell, M. J. (2021). Vacuolar H+-ATPase and Na+/K+-ATPase energize Na+ uptake mechanisms in the nuchal organ of the hyperregulating freshwater crustacean Daphnia magna. Journal of Experimental Biology, 224(12), jeb242205. https://doi.org/10.1242/jeb.242205

Morris, C., Sakarya, M., Koh, O., & O’Donnell, M. (2020). Alterations in Hemolymph Ion Concentrations and pH in Adult Daphnia magna in Response to Elevations in Major Ion Concentrations in Freshwater. Environmental Toxicology and Chemistry, 40(2), 366–379. https://doi.org/10.1002/etc.4919

OECD. (2004). Test No. 202: Daphnia sp. Acute Immobilisation Test. OECD Publishing. https://doi.org/10.1787/9789264069947-en. Retrieved March 8Th, 2026.

Ogle, D. H., Doll, J. C., Wheeler, A. P., & Dinno, A. (2026). FSA: Simple Fisheries Stock Assessment Methods. In CRAN: Contributed Packages. https://doi.org/10.32614/CRAN.package.FSA. Retrieved March 12Th, 2026.

Popp, T. E., Hermet, S., Fredette-Roman, J., McKeel, E., Zozaya, W., Baumlin, C., Charmantier, G., Lee, C. E., & Lorin-Nebel, C. (2024). Evolution of ion transporter Na+/K+-ATPase expression in the osmoregulatory maxillary glands of an invasive copepod. IScience, 27(7), 110278. https://doi.org/10.1016/j.isci.2024.110278

Rašković, B., Poleksić, V., Vuković, G., Špirović Trifunović, B., Božić, G., Ćupić Miladinović, D., Marković, Z., & Brkić, D. (2023). Acute and Subchronic Exposure of the Common Carp (Cyprinus carpio) to Herbicide S-Metolachlor. Water, 15(23), 4182. https://doi.org/10.3390/w15234182

Reilly, K., Ellis, L.-J. A., Davoudi, H. H., Supian, S., Maia, M. T., Silva, G. H., Guo, Z., Martinez, D. S. T., & Lynch, I. (2023). Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways. Frontiers in Toxicology, 5, 1178482. https://doi.org/10.3389/ftox.2023.1178482

Salvatore, M. M., Pappalardo, C., Suarez, E. G. P., Salvatore, F., Andolfi, A., Gesuele, R., Galdiero, E., Libralato, G., Guida, M., & Siciliano, A. (2024). Ecotoxicological and metabolomic investigation of chronic exposure of Daphnia magna (Straus, 1820) to yttrium environmental concentrations. Aquatic Toxicology, 276, 107117. https://doi.org/10.1016/j.aquatox.2024.107117

Schober, P., & Vetter, T. R. (2018). Survival Analysis and Interpretation of Time-to-Event Data: The Tortoise and the Hare. Anesthesia & Analgesia, 127(3), 792–798. https://doi.org/10.1213/ANE.0000000000003653

Seshan, V. E., & Whiting, K. (2023). Clinfun: Clinical Trial Design and Data Analysis Functions. In CRAN: Contributed Packages. https://doi.org/10.32614/CRAN.package.clinfun. Retrieved March 14Th, 2026.

Suarez, E. G. P., Revel, M., Libralato, G., Guida, M., & Heise, S. (2025). Sublethal and lethal toxicity assessment of lanthanum and gadolinium to Daphnia magna in a 7-day test method. Environmental Science and Pollution Research, 32(5), 2467–2478. https://doi.org/10.1007/s11356-024-35854-7

Sun, X., Arnott, S. E., & Little, A. G. (2024). Impacts of sequential salinity and heat stress are recovery time-specific in freshwater crustacean, Daphnia pulicaria. Ecotoxicology and Environmental Safety, 269, 115899. https://doi.org/10.1016/j.ecoenv.2023.115899

Therneau, T. M. (2021). Survival: Survival Analysis. In CRAN: Contributed Packages. https://doi.org/10.32614/CRAN.package.survival. Retrieved March 14Th, 2026.

Toma, C., Cappelli, C. I., Manganaro, A., Lombardo, A., Arning, J., & Benfenati, E. (2021). New Models to Predict the Acute and Chronic Toxicities of Representative Species of the Main Trophic Levels of Aquatic Environments. Molecules, 26(22), 6983. https://doi.org/10.3390/molecules26226983

Venables, W. N., & Ripley, B. D. (2002). Modern applied statistics with S (4th edition). Springer. 495 page. https://doi.org/10.1007/978-0-387-21706-2

Venâncio, C., Wijewardene, L., Ribeiro, R., & Lopes, I. (2023). Combined effects of two abiotic stressors (salinity and temperature) on a laboratory-simulated population of Daphnia longispina. Hydrobiologia, 850(14), 3197–3208. https://doi.org/10.1007/s10750-023-05249-9

Xiao, H., Jin, X., Wang, Z., Ye, Q., Li, W., Han, L., & Ding, J. (2025). Effects of Chronic Low-Salinity Stress on Growth, Survival, Antioxidant Capacity, and Gene Expression in Mizuhopecten yessoensis. Biology, 14(7), 759. https://doi.org/10.3390/biology14070759

Xu, E. G., Cheong, R. S., Liu, L., Hernandez, L. M., Azimzada, A., Bayen, S., & Tufenkji, N. (2020). Primary and Secondary Plastic Particles Exhibit Limited Acute Toxicity but Chronic Effects on Daphnia magna. Environmental Science & Technology, 54(11), 6859–6868. https://doi.org/10.1021/acs.est.0c00245

Yao, T., Masanja, F., Lu, J., Fu, S., Luo, W., Shija, V. M., Ye, L., & Zhao, L. (2025). Lasting impacts of rapid salinity change on physiological energetics of estuarine oysters (Crassostrea hongkongensis). Marine Environmental Research, 207, 107076. https://doi.org/10.1016/j.marenvres.2025.107076

Yuslan, A., Najuwa, S., Hagiwara, A., Ghaffar, M. A., Suhaimi, H., & Rasdi, N. W. (2021). Production Performance of Moina macrocopa (Straus 1820) (Crustacea, Cladocera) Cultured in Different Salinities: The Effect on Growth, Survival, Reproduction, and Fatty Acid Composition of the Neonates. Diversity, 13(3), 105. https://doi.org/10.3390/d13030105




DOI: https://doi.org/10.36987/jpbn.v12i3.9048

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